Bccip (NM_025392) Mouse Tagged ORF Clone

CAT#: MG204529

  • TrueORF®

Bccip (tGFP-tagged) - Mouse BRCA2 and CDKN1A interacting protein (Bccip)

ORF Plasmid: DDK tGFP



  "NM_025392" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,850.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 1110013J05Rik; TOK-1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG204529 representing NM_025392
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATCTAAGGCTAAGAAGCGAGCGGTGGGAAATGGAATTCAGCGGCCGCTGGGAGCCCCGGGCCAGC
GGGAGGAGGAAGAAGAAGAAGAGGATGAGGTGGAGGATGAGGAAGAAGACGAGGACGACAGTGACGAAGA
AGAGGATGAAGTTGACGAAATCGTGGACGAGGAAGTGAATATTGAGTTTGAAGCCTATTCCATCTCAGAT
AACGATTATGGCGGAATTAAGAAATTACTGCAACAGCTTTTTCTAAAAGCCCCCGTGAACACTGCCGAGC
TGACGAATCTCCTGATGCAGCAGAACCACATTGGGAGTGTGATTAAGCAAACAGATGTTTCAGAAGACAG
TGATGATGAAGTGGATGAAGATGAGATCTTTGGCTTCATAAGCCTTTTAAATTTGACTGAAAGAAAGGGG
ACTCAGTGTGCTGAACAAATTAAAGAGTTGGTTCTAAGCTTCTGTGAGAAGACCTGTGAGCAGAGCATGG
TTGAACAGCTGGACAAGCTTTTGAATGACACCAGCAAGCCTGTGGGCCTTCTGCTAAGTGAAAGATTCAT
TAACGTCCCTCCACAGATTGCCCTGCCCATGCACCAGCAGCTCCAGAAAGAACTGTCTGAGGCGCGAAGA
ACCAACAAGCCATGTGGGAAATGCTGCTTCTACCTGCTGATTAGTAAGACATTTATGGAAGCAGGAAAGT
CCAGTTCCAGAAAGAGGCAGGACAGCCTTCAGCAGGGGGCACTGATGTTTGCAAATGCAGAGGAAGAGTT
CTTCTATGAGAAGGCAATCTTGAAGTTCAGCTACTCGGTGCAGGGGGAGAGCGACACTCGTCTGGGAGGC
AGATGGTCCTTCGATGATGTTCCGATGACGCCTTTGCGGACTGTGATGGTAATTCCAGACGACAGGATGA
ATGAGATCATGGAAACCCTGAAAGACCATCTCTCCGTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG204529 representing NM_025392
Red=Cloning site Green=Tags(s)

MASKAKKRAVGNGIQRPLGAPGQREEEEEEEDEVEDEEEDEDDSDEEEDEVDEIVDEEVNIEFEAYSISD
NDYGGIKKLLQQLFLKAPVNTAELTNLLMQQNHIGSVIKQTDVSEDSDDEVDEDEIFGFISLLNLTERKG
TQCAEQIKELVLSFCEKTCEQSMVEQLDKLLNDTSKPVGLLLSERFINVPPQIALPMHQQLQKELSEARR
TNKPCGKCCFYLLISKTFMEAGKSSSRKRQDSLQQGALMFANAEEEFFYEKAILKFSYSVQGESDTRLGG
RWSFDDVPMTPLRTVMVIPDDRMNEIMETLKDHLSV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_025392
ORF Size 948 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_025392.2, NP_079668.2
RefSeq Size 1260 bp
RefSeq ORF 951 bp
Locus ID 66165
UniProt ID Q9CWI3
Gene Summary During interphase, required for microtubule organizing and anchoring activities. During mitosis, required for the organization and stabilization of the spindle pole (PubMed:28394342). May promote cell cycle arrest by enhancing the inhibition of CDK2 activity by CDKN1A. May be required for repair of DNA damage by homologous recombination in conjunction with BRCA2. May not be involved in non-homologous end joining (NHEJ) (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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