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Sgpl1 (NM_009163) Mouse Tagged ORF Clone
CAT＃: MG208957
- TrueORF®
Sgpl1 (tGFP-tagged) - Mouse sphingosine phosphate lyase 1 (Sgpl1)
ORF Plasmid: DDK
"NM_009163" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI428538; D10Xrf456; S1PL; Spl |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208957 representing NM_009163
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGGAACCGACCTCCTCAAGCTGAAGGACTTCGAGCCTTATTTGGAGATTTTGGAATCATATTCCA CAAAAGCCAAGAATTATGTGAATGGATATTGCACCAAATATGAGCCCTGGCAGCTCATTGCGTGGAGTGT CCTGTGTACTCTGCTGATAGTCTGGGTGTATGAGCTTATCTTCCAGCCAGAGAGTTTATGGTCTCGGTTT AAAAAAAAATTATTTAAGCTTATCAGGAAGATGCCATTTATTGGACGTAAGATCGAACAACAGGTGAGCA AAGCCAAGAAGGATCTTGTCAAGAACATGCCATTCCTAAAGGTGGACAAGGATTATGTGAAAACTCTGCC TGCTCAGGGTATGGGCACAGCTGAGGTTCTGGAGAGACTCAAGGAGTACAGCTCCATGGATGGTTCCTGG CAAGAAGGGAAAGCCTCAGGAGCTGTGTACAATGGGGAACCGAAGCTCACGGAGCTGCTGGTGCAGGCTT ATGGAGAATTCACGTGGAGCAATCCACTGCATCCAGATATCTTCCCTGGATTGCGGAAGTTAGAGGCAGA AATCGTTAGGATGACTTGTTCCCTCTTCAATGGGGGACCAGATTCCTGTGGATGTGTGACTTCTGGGGGA ACGGAAAGCATCCTGATGGCCTGCAAAGCTTACCGGGACTTGGCGTTAGAGAAGGGGATCAAAACTCCAG AAATTGTGGCTCCCGAGAGTGCCCATGCTGCATTCGACAAAGCAGCTCATTATTTTGGGATGAAGATTGT CCGAGTTGCACTGAAAAAGAACATGGAGGTGGATGTGCAGGCAATGAAGAGAGCCATCTCCAGGAACACA GCTATGCTGGTCTGTTCTACCCCACAGTTTCCTCATGGTGTGATGGATCCTGTCCCCGAAGTGGCCAAGT TGGCTGTCAGATATAAAATCCCACTCCATGTGGATGCTTGTCTGGGGGGCTTCCTCATTGTCTTCATGGA GAAAGCAGGGTACCCACTGGAGAAACCATTTGATTTCCGGGTGAAAGGTGTGACCAGCATTTCAGCAGAT ACTCATAAGTATGGCTATGCTCCTAAAGGTTCATCAGTGGTGATGTACTCTAACGAGAAGTACAGGACGT ACCAGTTCTTTGTTGGTGCAGACTGGCAAGGTGGTGTCTACGCATCTCCAAGCATAGCTGGCTCACGGCC TGGTGGCATCATTGCAGCCTGTTGGGCCGCCTTGATGCACTTCGGTGAGAACGGCTATGTTGAAGCTACC AAACAGATCATCAAAACTGCTCGCTTCCTGAAGTCAGAACTGGAAAACATCAAAAACATCTTCATTTTCG GTGATCCTCAATTGTCAGTTATTGCTCTGGGATCCAACGATTTTGACATTTACCGACTATCTAATATGAT GTCTGCTAAGGGGTGGAATTTTAACTACCTGCAGTTCCCAAGAAGCATTCATTTCTGCATTACGTTAGTA CATACTCGGAAGCGAGTGGCGATCCAGTTCCTAAAGGATATCCGGGAATCAGTCACACAAATCATGAAGA ATCCTAAAGCTAAGACCACAGGAATGGGTGCCATCTATGGCATGGCCCAGGCAACCATTGACAGGAAGCT GGTTGCAGAAATATCCTCCGTCTTCTTGGACTGCCTTTATACTACGGACCCCGTGACTCAGGGCAACCAG ATGAACGGTTCTCCAAAGCCCCGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208957 representing NM_009163
Red=Cloning site Green=Tags(s) MPGTDLLKLKDFEPYLEILESYSTKAKNYVNGYCTKYEPWQLIAWSVLCTLLIVWVYELIFQPESLWSRF KKKLFKLIRKMPFIGRKIEQQVSKAKKDLVKNMPFLKVDKDYVKTLPAQGMGTAEVLERLKEYSSMDGSW QEGKASGAVYNGEPKLTELLVQAYGEFTWSNPLHPDIFPGLRKLEAEIVRMTCSLFNGGPDSCGCVTSGG TESILMACKAYRDLALEKGIKTPEIVAPESAHAAFDKAAHYFGMKIVRVALKKNMEVDVQAMKRAISRNT AMLVCSTPQFPHGVMDPVPEVAKLAVRYKIPLHVDACLGGFLIVFMEKAGYPLEKPFDFRVKGVTSISAD THKYGYAPKGSSVVMYSNEKYRTYQFFVGADWQGGVYASPSIAGSRPGGIIAACWAALMHFGENGYVEAT KQIIKTARFLKSELENIKNIFIFGDPQLSVIALGSNDFDIYRLSNMMSAKGWNFNYLQFPRSIHFCITLV HTRKRVAIQFLKDIRESVTQIMKNPKAKTTGMGAIYGMAQATIDRKLVAEISSVFLDCLYTTDPVTQGNQ MNGSPKPR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009163 |
| ORF Size | 1704 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009163.4 |
| RefSeq Size | 4108 bp |
| RefSeq ORF | 1707 bp |
| Locus ID | 20397 |
| UniProt ID | Q8R0X7 |
| Gene Summary | Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine (PubMed:9464245, PubMed:20097939). Elevates stress-induced ceramide production and apoptosis (PubMed:9464245). Required for global lipid homeostasis in liver and cholesterol homeostasis in fibroblasts (PubMed:20097939, PubMed:28262793). Involved in the regulation of pro-inflammatory response and neutrophil trafficking (PubMed:21173151). Modulates neuronal autophagy via phosphoethanolamine production which regulates accumulation of aggregate-prone proteins such as APP (PubMed:28521611). Seems to play a role in establishing neuronal contact sites and axonal maintenance (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202842 | Sgpl1 (untagged) - Mouse sphingosine phosphate lyase 1 (Sgpl1), (10ug) |
CNY 2,000.00 |
|
| MR208957 | Sgpl1 (Myc-DDK-tagged) - Mouse sphingosine phosphate lyase 1 (Sgpl1) |
CNY 4,240.00 |
|
| MR208957L3 | Lenti ORF clone of Sgpl1 (Myc-DDK-tagged) - Mouse sphingosine phosphate lyase 1 (Sgpl1) |
CNY 6,460.00 |
|
| MR208957L4 | Lenti ORF clone of Sgpl1 (mGFP-tagged) - Mouse sphingosine phosphate lyase 1 (Sgpl1) |
CNY 6,460.00 |
