United States
Germany
Japan
United Kingdom
China
Btk (NM_013482) Mouse Tagged ORF Clone
CAT＃: MG209829
- TrueORF®
Btk (tGFP-tagged) - Mouse Bruton agammaglobulinemia tyrosine kinase (Btk)
ORF Plasmid: DDK
"NM_013482" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI528679; xid |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209829 representing NM_013482
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCAGTGATACTGGAGAGCATCTTTCTGAAGCGCTCCCAGCAGAAAAAGAAAACATCACCTTTAA ACTTCAAGAAGCGCCTGTTTCTCTTGACTGTACACAAACTTTCATACTATGAATATGACTTTGAACGTGG GAGAAGAGGCAGTAAGAAAGGTTCAATAGATGTTGAGAAGATCACCTGTGTTGAAACAGTAATTCCTGAA AAAAATCCCCCACCAGAAAGACAGATTCCGAGGAGAGGTGAGGAGTCTAGTGAAATGGAACAGATTTCAA TCATTGAAAGGTTCCCGTACCCATTCCAGGTTGTATATGATGAAGGACCTCTCTATGTTTTCTCCCCAAC TGAAGAGCTGAGAAAGCGCTGGATTCACCAGCTCAAAAATGTAATCCGGTGCAATAGTGACCTGGTACAG AAATACCATCCTTGCTTCTGGATTGATGGACAGTATCTCTGCTGCTCTCAGACAGCCAAGAATGCTATGG GCTGCCAAATTTTGGAGAACAGGAATGGAAGCTTAAAACCTGGGAGTTCTCATCGAAAAACGAAAAAGCC TCTTCCCCCTACCCCAGAGGAAGATCAGATCTTGAAAAAACCGCTTCCCCCGGAGCCAACAGCAGCACCA ATCTCCACAACCGAGCTGAAAAAGGTCGTGGCCCTTTATGATTACATGCCAATGAACGCAAATGACTTAC AATTGCGAAAGGGCGAGGAGTATTTTATCCTGGAGGAGAGCAACCTACCGTGGTGGCGAGCACGAGATAA AAATGGGCAGGAAGGCTACATCCCAAGTAACTATATCACTGAAGCTGAGGACTCCATAGAGATGTATGAG TGGTATTCCAAGCACATGACTCGAAGTCAAGCTGAGCAACTGCTAAAGCAAGAGGGGAAAGAAGGAGGTT TCATTGTCGGAGACTCCAGCAAAGCTGGAAAATACACCGTGTCTGTGTTTGCTAAATCTACTGGGGAGCC TCAAGGGGTGATCCGCCATTACGTTGTGTGTTCCACGCCACAGAGCCAGTATTACCTGGCTGAGAAACAC CTCTTCAGCACCATCCCTGAGCTCATTAACTACCATCAACACAACTCTGCAGGCCTCATATCCAGGCTGA AATATCCTGTGTCTAAACAAAACAAAAACGCGCCTTCTACTGCAGGCCTGGGCTATGGATCATGGGAAAT TGATCCAAAGGACCTCACCTTCTTGAAGGAGCTTGGGACTGGACAATTCGGTGTCGTGAAATATGGGAAG TGGAGGGGCCAATATGATGTGGCCATCAAGATGATCAGAGAAGGTTCCATGTCGGAGGATGAATTCATTG AAGAAGCCAAAGTCATGATGAATCTTTCCCATGAGAAGCTGGTGCAGTTGTATGGCGTCTGCACCAAACA ACGCCCCATCTTCATCATCACCGAGTACATGGCTAATGGCTGCCTCTTGAACTACCTGAGGGAGATGCGG CACCGCTTCCAGACACAGCAGCTGCTTGAGATGTGCAAAGATGTCTGTGAAGCAATGGAATACTTGGAGT CGAAGCAGTTCCTTCACAGAGACCTGGCAGCTCGAAACTGTTTGGTAAACGATCAAGGAGTTGTGAAAGT ATCTGACTTTGGCCTGTCTAGGTATGTCCTTGATGATGAGTACACCAGCTCTGTAGGCTCCAAGTTTCCA GTCCGGTGGTCTCCACCAGAAGTGCTTATGTATAGCAAGTTCAGCAGCAAATCTGACATCTGGGCTTTTG GGGTTTTAATGTGGGAGATCTACTCCCTGGGGAAGATGCCGTATGAGAGATTTACTAACAGTGAGGCAGC AGAACACATTGCTCAAGGCTTACGTCTCTACAGGCCTCATCTGGCATCAGAGAGGGTATATACCATCATG TACAGCTGCTGGCACGAGAAAGCAGATGAACGTCCTAGTTTCAAAATTCTCTTGAGTAACATTCTAGATG TGATGGATGAAGAATCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209829 representing NM_013482
Red=Cloning site Green=Tags(s) MAAVILESIFLKRSQQKKKTSPLNFKKRLFLLTVHKLSYYEYDFERGRRGSKKGSIDVEKITCVETVIPE KNPPPERQIPRRGEESSEMEQISIIERFPYPFQVVYDEGPLYVFSPTEELRKRWIHQLKNVIRCNSDLVQ KYHPCFWIDGQYLCCSQTAKNAMGCQILENRNGSLKPGSSHRKTKKPLPPTPEEDQILKKPLPPEPTAAP ISTTELKKVVALYDYMPMNANDLQLRKGEEYFILEESNLPWWRARDKNGQEGYIPSNYITEAEDSIEMYE WYSKHMTRSQAEQLLKQEGKEGGFIVGDSSKAGKYTVSVFAKSTGEPQGVIRHYVVCSTPQSQYYLAEKH LFSTIPELINYHQHNSAGLISRLKYPVSKQNKNAPSTAGLGYGSWEIDPKDLTFLKELGTGQFGVVKYGK WRGQYDVAIKMIREGSMSEDEFIEEAKVMMNLSHEKLVQLYGVCTKQRPIFIITEYMANGCLLNYLREMR HRFQTQQLLEMCKDVCEAMEYLESKQFLHRDLAARNCLVNDQGVVKVSDFGLSRYVLDDEYTSSVGSKFP VRWSPPEVLMYSKFSSKSDIWAFGVLMWEIYSLGKMPYERFTNSEAAEHIAQGLRLYRPHLASERVYTIM YSCWHEKADERPSFKILLSNILDVMDEES TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013482 |
| ORF Size | 1977 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013482.1, NP_038510.1 |
| RefSeq Size | 2485 bp |
| RefSeq ORF | 1980 bp |
| Locus ID | 12229 |
| UniProt ID | P35991 |
| Gene Summary | Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense. Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK plays also a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes. There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202055 | Btk (untagged) - Mouse Bruton agammaglobulinemia tyrosine kinase (Btk), (10ug) |
CNY 2,000.00 |
|
| MR209829 | Btk (Myc-DDK-tagged) - Mouse Bruton agammaglobulinemia tyrosine kinase (Btk) |
CNY 4,560.00 |
|
| MR209829L3 | Lenti ORF clone of Btk (Myc-DDK-tagged) - Mouse Bruton agammaglobulinemia tyrosine kinase (Btk) |
CNY 6,460.00 |
|
| MR209829L4 | Lenti ORF clone of Btk (mGFP-tagged) - Mouse Bruton agammaglobulinemia tyrosine kinase (Btk) |
CNY 6,460.00 |
