United States
Germany
Japan
United Kingdom
China
Padi4 (NM_011061) Mouse Tagged ORF Clone
CAT＃: MG216758
- TrueORF®
Padi4 (tGFP-tagged) - Mouse peptidyl arginine deiminase type IV (Padi4), (10ug)
ORF Plasmid: DDK
"NM_011061" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 9,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Pad4; Pdi4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG216758 representing NM_011061
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAAGGCGCGGTGATCCACGTGGCCCCCGAGCAGCCCACTCACGCCGTGTGTGTGGTGGGCACAG CGACCCCGCTGGATGTCCGCGGTTCTGCTCCTAAGGGCTACACAACCTTCGGCATCACAGCCTCTCCAGG AGTCATCGTAGATGTCATCCATGGTCCTCCAGTCAAGAAGAGTACCATGGGGGCCTCCAAATGGCCTCTG GACCCTGAGCTGGAGGTGACCCTACAGGTGAAAGCAGCCAGCAGCAGAACAGATGATGAAAAGGTTCGAG TTTCATACTATGGACCCAAGACCTCCCCAGTCCAAGCCCTGATCTACATCACTGGGGTGGAACTGTCCCT GAGCGCAGATGTCACCCGCACAGGCAGAGTGAAGCCAGCCCAAGCCGGGAAGGATCAGAGCACCTGGACC TGGGGCCCGGGCGGCCGTGGCGCCATCCTGTTGGTGAACTGTGACAAAGAGGACCCTCAGGCCTCCGGAA TGGACTTTGAGGATGACAAGATCTTGGACAACAAAGACCTGCAGGACATGTCTCCAATGACCCTAAGCAC GAAGACGCCCAAAGACTTCTTTGAAAAGTATCAGCTGGTGTTGGAGGTGCCCAAGGCCAAGATGAACAGA GTGAGAGTCTTCCGGGCCACACGGGGCAAACTGCCGTCCCGGTACAAGGTGGCCCTGGGACCACAACAGT TCTCGTATTGCCTGGAGCTGCCCGGCGGCCAGCACAGCACAGACTTCTATGTGGAAGGCCTTGCTTTCCC AGACGCAGACTTCAAAGGGCTCATTCCCCTCACCATCTCCCTGCTGGACAAGTCTAACCCGGAGCTCCCC GAGGCCCTGGTGTTCCAAGACAGTGTGACGTTCCGTGTGGCCCCCTGGATCATGACCCCCAACACTCAGC CCCCCCAGGAGGTGTACGTGTGCAGGGTTTCTGACAATGAAGACTTCCTAAAGTCACTAGCTACTCTGAC CAAGAAAGCCAAGTGCAAGCTGACTGTGTGCCCCGAGGAGGAGAATATAGATGACCAATGGATGCAGGAC GAAATGGAGATTGGCTACATCCAGGCCCCACACAAGACGCTGCCTGTGGTCTTTGACTCCCCGAGGGACA GAGGCCTGAAGGATTTCCCTGTCAAGCGAGTTATGGGTCCAAATTTTGGCTATGTGACCCGAAAGCTCTA TATGTCAGAGCTCACTGGGCTGGATGCCTTTGGGAACCTGGAGGTGAGTCCCCCAGTCACTGTCAGAGGG AAGGAGTACCCACTGGGCAGAATTCTCATCGGGAATAGCGGTTACTCCAGCAGCGAGAGCCGGGACATGC ACCAGGCCCTGCAGGACTTCCTGAGCGCCCAGCAGGTGCAGGCCCCCGTGAGGCTCTTCTCCGATTGGCT CTTTGTGGGTCACGTGGATGAGTTCTTGAGCTTTGTCCCAGCGCGGGACAAGCAGGGTTTTCGGCTGCTG CTGTCCAGCCCCAGAGCTTGCTATCAGCTGTTCCAGGAGCTACAGAGCCAGGGCCACGGGGAGGCGACAC TGTTCGAAGGACTCAAGAGGAAAAGGCAGACAATCAATGAAATTCTGTCCAACAAGAAATTAAGAGACCA GAATGCCTATGTGGAGAGCTGTATCGACTGGAACCGGGCGGTGCTGAAGCGGGAGCTGGGCCTGGCAGAG GGTGACATCATCGACATTCCGCAGCTCTTCAAGCTCGCGGGGAACTCCAGAGGGAACTCTAAGGCCCAGG CCTTCTTCCCAAACATGGTGAACATGCTGGTCCTGGGCAAGTACCTGGGCATCCCCAAGCCCTTTGGGCC CATCATCGATGGCCACTGCTGCCTGGAGGAGGAGGTGCGTTCCCACCTGGAGCCGCTGGGTCTGCACTGC ACCTTCATCAACGACTTCTACACCTACCACGTGTACAACGGGGAGGTTCACTGTGGCACCAATGTGCGCA GGAAGCCCTTCACCTTCAAGTGGTGGCACATGGTGCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG216758 representing NM_011061
Red=Cloning site Green=Tags(s) MAQGAVIHVAPEQPTHAVCVVGTATPLDVRGSAPKGYTTFGITASPGVIVDVIHGPPVKKSTMGASKWPL DPELEVTLQVKAASSRTDDEKVRVSYYGPKTSPVQALIYITGVELSLSADVTRTGRVKPAQAGKDQSTWT WGPGGRGAILLVNCDKEDPQASGMDFEDDKILDNKDLQDMSPMTLSTKTPKDFFEKYQLVLEVPKAKMNR VRVFRATRGKLPSRYKVALGPQQFSYCLELPGGQHSTDFYVEGLAFPDADFKGLIPLTISLLDKSNPELP EALVFQDSVTFRVAPWIMTPNTQPPQEVYVCRVSDNEDFLKSLATLTKKAKCKLTVCPEEENIDDQWMQD EMEIGYIQAPHKTLPVVFDSPRDRGLKDFPVKRVMGPNFGYVTRKLYMSELTGLDAFGNLEVSPPVTVRG KEYPLGRILIGNSGYSSSESRDMHQALQDFLSAQQVQAPVRLFSDWLFVGHVDEFLSFVPARDKQGFRLL LSSPRACYQLFQELQSQGHGEATLFEGLKRKRQTINEILSNKKLRDQNAYVESCIDWNRAVLKRELGLAE GDIIDIPQLFKLAGNSRGNSKAQAFFPNMVNMLVLGKYLGIPKPFGPIIDGHCCLEEEVRSHLEPLGLHC TFINDFYTYHVYNGEVHCGTNVRRKPFTFKWWHMVP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011061 |
| ORF Size | 1998 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011061.2, NP_035191.2 |
| RefSeq Size | 2640 bp |
| RefSeq ORF | 2001 bp |
| Locus ID | 18602 |
| UniProt ID | Q9Z183 |
| Gene Summary | Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220244 | Padi4 (untagged) - Mouse peptidyl arginine deiminase, type IV (Padi4), (10ug) |
CNY 4,888.00 |
|
| MR216758 | Padi4 (Myc-DDK-tagged) - Mouse peptidyl arginine deiminase, type IV (Padi4) |
CNY 7,440.00 |
|
| MR216758L3 | Lenti ORF clone of Padi4 (Myc-DDK-tagged) - Mouse peptidyl arginine deiminase, type IV (Padi4) |
CNY 7,700.00 |
|
| MR216758L4 | Lenti ORF clone of Padi4 (mGFP-tagged) - Mouse peptidyl arginine deiminase, type IV (Padi4) |
CNY 9,840.00 |
