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Eno3 (NM_001136062) Mouse Tagged ORF Clone
CAT＃: MG222148
- TrueORF®
Eno3 (tGFP-tagged) - Mouse enolase 3 beta muscle (Eno3) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001136062" in other vectors (3)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Eno; Eno-3; MSE |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222148 representing NM_001136062
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCATGCAAAAAATCTTCGCCCGGGAAATCCTGGACTCCAGGGGCAACCCCACGGTGGAGGTGGACC TGCACACAGCCAAGGGTCGATTCCGAGCAGCTGTGCCCAGTGGAGCTTCCACGGGTATCTATGAAGCACT GGAACTCCGAGATGGAGACAAAGCACGATACCTGGGGAAAGGAGTGCTGAAGGCTGTGGAACACATCAAC AAGACTCTAGGTCCTGCTCTGCTGGAAAAGAAACTAAGTGTTGTGGATCAAGAAAAAGTTGACAAGTTCA TGATTGAGCTGGACGGGACCGAGAATAAGTCCAAGTTTGGGGCCAACGCCATCCTGGGTGTGTCCCTGGC TGTCTGCAAGGCTGGAGCAGCTGAGAAAGGGGTCCCTCTCTACCGACACATCGCAGATCTTGCAGGCAAT CCCGACCTCGTACTCCCTGTGCCTGCCTTTAATGTGATCAACGGCGGCTCTCATGCTGGAAACAAGCTGG CCATGCAGGAGTTCATGATTCTGCCAGTGGGAGCCAGCTCTTTCAAGGAAGCCATGCGCATCGGCGCTGA GGTCTACCACCACCTCAAGGGGGTCATCAAGGCCAAGTATGGGAAGGACGCCACCAACGTGGGGGATGAG GGTGGCTTTGCACCCAACATCCTGGAGAACAATGAGGCCCTGGAGCTGCTAAAGACAGCCATCCAGGCAG CCGGTTACCCGGACAAGGTGGTGATCGGCATGGATGTAGCTGCGTCTGAATTCTACCGCAACGGCAAGTA TGATCTGGACTTCAAGTCACCCGATGACCCTGCCAGGCACATCAGTGGGGAGAAGCTTGGGGAGCTGTAC AAGAACTTCATCCAGAACTATCCCGTGGTCTCCATTGAGGACCCCTTTGACCAGGATGACTGGGCCACAT GGACCTCATTCCTCTCTGGGGTGGACATCCAGATTGTGGGAGATGACCTCACGGTAACCAACCCCAAGAG GATTGCTCAGGCTGTGGAGAAGAAGGCCTGCAATTGCCTGCTCCTGAAGGTCAACCAGATCGGCTCCGTG ACGGAGTCCATCCAGGCCTGTAAACTTGCACAATCTAATGGCTGGGGAGTGATGGTGAGCCACCGCTCTG GGGAGACCGAAGACACTTTCATCGCTGACCTTGTGGTGGGACTCTGCACAGGACAGATCAAGACTGGTGC TCCCTGCCGTTCAGAGCGTCTGGCAAAATACAACCAGCTTATGAGGATTGAGGAGGCTCTTGGGGACAAA GCTGTCTTTGCTGGAAGAAAGTTCCGTAATCCAAAGGCCAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222148 representing NM_001136062
Red=Cloning site Green=Tags(s) MAMQKIFAREILDSRGNPTVEVDLHTAKGRFRAAVPSGASTGIYEALELRDGDKARYLGKGVLKAVEHIN KTLGPALLEKKLSVVDQEKVDKFMIELDGTENKSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGN PDLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMRIGAEVYHHLKGVIKAKYGKDATNVGDE GGFAPNILENNEALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGKYDLDFKSPDDPARHISGEKLGELY KNFIQNYPVVSIEDPFDQDDWATWTSFLSGVDIQIVGDDLTVTNPKRIAQAVEKKACNCLLLKVNQIGSV TESIQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDK AVFAGRKFRNPKAK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001136062 |
| ORF Size | 1302 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001136062.2 |
| RefSeq Size | 1440 bp |
| RefSeq ORF | 1305 bp |
| Locus ID | 13808 |
| UniProt ID | P21550 |
| Gene Summary | This gene encodes one of the three enolase isoenzymes found in vertebrates. Enolase is a dimeric enzyme that converts 2-phosphoglycerate to phosphoenolpyruvate as part of the glycolytic pathway. This isozyme is found in skeletal muscle where it is involved in muscle development and regeneration. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR222148 | Eno3 (Myc-DDK-tagged) - Mouse enolase 3, beta muscle (Eno3), transcript variant 2 |
CNY 3,330.00 |
|
| MR222148L3 | Lenti ORF clone of Eno3 (Myc-DDK-tagged) - Mouse enolase 3, beta muscle (Eno3), transcript variant 2 |
CNY 5,230.00 |
|
| MR222148L4 | Lenti ORF clone of Eno3 (mGFP-tagged) - Mouse enolase 3, beta muscle (Eno3), transcript variant 2 |
CNY 5,230.00 |
