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Trim25 (NM_009546) Mouse Tagged ORF Clone
CAT＃: MG223266
- TrueORF®
Trim25 (tGFP-tagged) - Mouse tripartite motif-containing 25 (Trim25), (10ug)
ORF Plasmid: DDK
"NM_009546" in other vectors (4)
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CNY 7,030.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA960166; AL022677; EFP; Zfp147 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG223266 representing NM_009546
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCTGAATCCTCTGGCCGAGGAGCTGTCCTGCTCCGTCTGCCTGGAGCTCTTCAAGGAACCTG TCACCACTCCGTGCGGCCACAATTTCTGCATGTCATGCCTGGATGAGACGTGGGTCGTCCAGGGCCCGCC GTACCGATGCCCGCAATGTCGCAAAGTGTACCAGGTGCGCCCGCAGCTGCAAAAGAACACGGTAATGTGC GCGGTGGTGGAGCAGTTCCTGCAGGCCGAGCAGGCGCGGACCCCGGTGGACGACTGGACGCCTCCTGCCC GCTTCTCCGCCTCCAGCGCAGCCACCCAGGTGGCCTGCGACCACTGCCTGACGGAGATCGCAGTGAAGAC GTGCCTCGTGTGCATGGCCTCTTTCTGCCAGGAGCACTTGAGGCCTCACTTCGACAGTCCGGCCTTCCAG GATCACCCCCTACAGTCGCCCATTCGTGACCTTCTGCGCCGCAAATGTACCCAGCACAACCGCCTTCGGG AATTGTTTTGTCCCGAGCATGGCGAGTGCATCTGCCATATCTGCTTGGTAGAGCACAAGACCTGCTCCCC TACGACCCTAAGTCAAGCCAGCGCCGACCTGGAGTACAAACTGAGGAATAAACTCACTATCATGCACAGC CACATCAATGGGGCAACCAAAGCATTGGAGGATGTGAGATCCAAGCAACAGTGTGTGCAGGATTCTATGA AGAGGAAGATGGAACAGCTGAGACAGGAGTATATGGAAATGAAGGCTGTCATCGACGCAGCAGAGACCAG CTCCTTGCGCAAGTTAAAGGAGGAGGAGAAGAGAGTCTACGGAAAATTCGACACCATCTACCAGGTTCTC GTCAAGAAGAAGAGTGAGATGCAGAAACTGAAGGCAGAGGTTGAGCTCATTATGGACAAAGGGGATGAGT TTGAGTTTCTGGAGAAAGCTGCAAAATTGCAAGGAGAGTCAACAAAACCAGTCTACATCCCTAAGATAGA TCTGGATCATGATTTGATAATGGGAATCTACCAAGGGGCGGCGGACCTCAAGAGTGAACTGAAGCACTCC ATTAAGAAGCTACAGAAGAAGTCTGAGGAGCACAATGGCTCAGGTAACAAGGGAGACCAGACACAGTCCA CTTTTAAACCCGTACAACCTTCGAAGAAGACCATTCAAGAAAAGAAGACCAAGAAAACCCCTGTTGCTCC CGGCCCTCCCTCCCACTTCTCACCTAACAAGTTGCCCACCTTTGGAGCTCCTGGCCAATCACTGGATTCA AAAGCAACTTCCCCTGATGCCGCACCCAAAGCCAGTGCTGCACAGCCAGACTCTGTAGGAGTCAAGGCTA AGGTGCTAGAAAACTTCTTAACCAAGTCCAGAACGGAGCTTCTGGAGTATTTCGTGAAAGTCATCTTCGA CTACAATACCGCCCACAACAAAGTGTCTCTGTCAAACAAGTACACCACCGCCTCTGTGTCCGATGGGCTC CAGCACTACCGGTCCCATCCCCAGAGGTTCACCTACTGCTCTCAGGTCCTGGGGCTGCACTGCTACAAGA ACGGCATTCACTACTGGGAGGTGGAACTACAGAAGAACAACTTCTGCGGCGTAGGCATCTGCTACGGCAG CATGGAACGGCAGGGCCCCGAGAGCCGGCTGGGCCGCAACCCCAACTCATGGTGTGTGGAGTGGTTCAAT AACAAGATCTCTGCCTGGCACAACAACGTGGAGAAGACTCTGCCCTCCACCAAGGCCACTCGAGTCGGCG TGCTGCTCAACTGTGACCACGGCTTCGTCATTTTCTTTGCGGTCACCGAAAAGGTGCACCTGATGTACAA GTTCAAGGTGGACTTCACCGAAGCTCTGTACCCAGCCTTCTGGGTGTTCTCGGCCGGTACCACCCTCTCT ATCTGCTCCAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223266 representing NM_009546
Red=Cloning site Green=Tags(s) MAELNPLAEELSCSVCLELFKEPVTTPCGHNFCMSCLDETWVVQGPPYRCPQCRKVYQVRPQLQKNTVMC AVVEQFLQAEQARTPVDDWTPPARFSASSAATQVACDHCLTEIAVKTCLVCMASFCQEHLRPHFDSPAFQ DHPLQSPIRDLLRRKCTQHNRLRELFCPEHGECICHICLVEHKTCSPTTLSQASADLEYKLRNKLTIMHS HINGATKALEDVRSKQQCVQDSMKRKMEQLRQEYMEMKAVIDAAETSSLRKLKEEEKRVYGKFDTIYQVL VKKKSEMQKLKAEVELIMDKGDEFEFLEKAAKLQGESTKPVYIPKIDLDHDLIMGIYQGAADLKSELKHS IKKLQKKSEEHNGSGNKGDQTQSTFKPVQPSKKTIQEKKTKKTPVAPGPPSHFSPNKLPTFGAPGQSLDS KATSPDAAPKASAAQPDSVGVKAKVLENFLTKSRTELLEYFVKVIFDYNTAHNKVSLSNKYTTASVSDGL QHYRSHPQRFTYCSQVLGLHCYKNGIHYWEVELQKNNFCGVGICYGSMERQGPESRLGRNPNSWCVEWFN NKISAWHNNVEKTLPSTKATRVGVLLNCDHGFVIFFAVTEKVHLMYKFKVDFTEALYPAFWVFSAGTTLS ICSK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009546 |
| ORF Size | 1902 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009546.2, NP_033572.2 |
| RefSeq Size | 5596 bp |
| RefSeq ORF | 1905 bp |
| Locus ID | 217069 |
| UniProt ID | Q61510 |
| Gene Summary | Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58 and IFIH1. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Mediates 'Lys-63'-linked polyubiquitination of IFIH1. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219941 | Trim25 (untagged) - Mouse tripartite motif-containing 25 (Trim25), (10ug) |
CNY 5,200.00 |
|
| MR223266 | Trim25 (Myc-DDK-tagged) - Mouse tripartite motif-containing 25 (Trim25) |
CNY 6,370.00 |
|
| MR223266L3 | Lenti ORF clone of Trim25 (Myc-DDK-tagged) - Mouse tripartite motif-containing 25 (Trim25) |
CNY 8,270.00 |
|
| MR223266L4 | Lenti ORF clone of Trim25 (mGFP-tagged) - Mouse tripartite motif-containing 25 (Trim25) |
CNY 8,270.00 |
