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Hexb (NM_010422) Mouse Tagged ORF Clone
CAT＃: MG226722
- TrueORF®
Hexb (tGFP-tagged) - Mouse hexosaminidase B (Hexb), (10ug)
ORF Plasmid: DDK
"NM_010422" in other vectors (4)
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CNY 5,890.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226722 representing NM_010422
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCAGTCCCCGCGTAGCGCCCCCGGGCTGCTGCTGCTGCAGGCGCTGGTGTCGCTAGTGTCGCTGG CCCTAGTGGCCCCGGCCCGACTGCAACCTGCGCTATGGCCCTTCCCGCGCTCGGTGCAGATGTTCCCGCG GCTGTTGTACATCTCCGCGGAGGACTTCAGCATCGACCACAGTCCCAATTCCACAGCGGGCCCTTCCTGC TCGCTGCTACAGGAGGCGTTTCGGCGATATTACAACTATGTTTTTGGTTTCTACAAGAGACATCATGGCC CTGCTAGATTTCGAGCTGAGCCACAGTTGCAGAAGCTCCTGGTCTCCATTACCCTCGAGTCAGAGTGCGA GTCCTTCCCTAGTCTGTCTTCAGATGAAACCTATTCTCTGCTTGTACAAGAACCAGTAGCCGTCCTCAAG GCCAACAGCGTTTGGGGAGCGTTACGAGGTTTAGAGACGTTTAGCCAGTTAGTTTACCAAGACTCTTTCG GGACTTTCACCATCAATGAATCCAGTATAGCTGATTCTCCAAGATTCCCTCATAGAGGAATTTTAATTGA TACATCTAGACACTTCCTGCCTGTGAAGACAATTTTAAAAACTCTGGATGCCATGGCTTTTAATAAGTTT AATGTTCTTCACTGGCACATAGTGGACGACCAGTCTTTCCCTTATCAGAGTACCACTTTTCCTGAGCTAA GCAATAAGGGAAGCTACTCTTTGTCTCATGTCTATACACCAAACGATGTCCGGATGGTGCTGGAGTACGC CCGGCTCCGAGGGATTCGAGTCATACCAGAATTTGATACCCCTGGCCATACACAGTCTTGGGGCAAAGGA CAGAAAAACCTTCTAACTCCATGTTACAATCAAAAAACTAAAACTCAAGTGTTTGGGCCTGTAGACCCAA CTGTAAACACAACGTATGCATTCTTTAACACATTTTTCAAAGAAATCAGCAGTGTGTTTCCAGATCAGTT CATCCACTTGGGAGGAGATGAAGTAGAATTTCAATGTTGGGCATCAAATCCAAACATCCAAGGTTTCATG AAGAGAAAGGGCTTTGGCAGCGATTTTAGAAGACTAGAATCCTTTTATATTAAAAAGATTTTGGAAATTA TTTCATCCTTAAAGAAGAACTCCATTGTTTGGCAAGAAGTTTTTGATGATAAGGTGGAGCTTCAGCCGGG CACAGTAGTCGAAGTGTGGAAGAGTGAGCATTATTCATATGAGCTAAAGCAAGTCACAGGCTCTGGCTTC CCTGCCATCCTTTCTGCTCCTTGGTACTTAGACCTGATCAGCTATGGGCAAGACTGGAAAAACTACTACA AAGTTGAGCCCCTTAATTTTGAAGGCTCTGAGAAGCAGAAACAACTTGTTATTGGTGGAGAAGCTTGCCT GTGGGGAGAATTTGTGGATGCAACTAACCTTACTCCAAGATTATGGCCTCGAGCAAGCGCTGTTGGTGAG AGACTCTGGAGCCCTAAAACTGTCACTGACCTAGAAAATGCCTACAAACGACTGGCCGTGCACCGCTGCA GAATGGTCAGCCGTGGAATAGCTGCACAACCTCTCTATACTGGATACTGTAACTATGAGAATAAAATA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226722 representing NM_010422
Red=Cloning site Green=Tags(s) MPQSPRSAPGLLLLQALVSLVSLALVAPARLQPALWPFPRSVQMFPRLLYISAEDFSIDHSPNSTAGPSC SLLQEAFRRYYNYVFGFYKRHHGPARFRAEPQLQKLLVSITLESECESFPSLSSDETYSLLVQEPVAVLK ANSVWGALRGLETFSQLVYQDSFGTFTINESSIADSPRFPHRGILIDTSRHFLPVKTILKTLDAMAFNKF NVLHWHIVDDQSFPYQSTTFPELSNKGSYSLSHVYTPNDVRMVLEYARLRGIRVIPEFDTPGHTQSWGKG QKNLLTPCYNQKTKTQVFGPVDPTVNTTYAFFNTFFKEISSVFPDQFIHLGGDEVEFQCWASNPNIQGFM KRKGFGSDFRRLESFYIKKILEIISSLKKNSIVWQEVFDDKVELQPGTVVEVWKSEHYSYELKQVTGSGF PAILSAPWYLDLISYGQDWKNYYKVEPLNFEGSEKQKQLVIGGEACLWGEFVDATNLTPRLWPRASAVGE RLWSPKTVTDLENAYKRLAVHRCRMVSRGIAAQPLYTGYCNYENKI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010422 |
| ORF Size | 1608 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010422.2, NP_034552.1 |
| RefSeq Size | 1922 bp |
| RefSeq ORF | 1611 bp |
| Locus ID | 15212 |
| UniProt ID | P20060 |
| Gene Summary | Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217566 | Hexb (untagged) - Mouse hexosaminidase B (Hexb), (10ug) |
CNY 6,000.00 |
|
| MR226722 | Hexb (Myc-DDK-tagged) - Mouse hexosaminidase B (Hexb) |
CNY 5,984.00 |
|
| MR226722L3 | Lenti ORF clone of Hexb (Myc-DDK-tagged) - Mouse hexosaminidase B (Hexb) |
CNY 7,220.00 |
|
| MR226722L4 | Lenti ORF clone of Hexb (mGFP-tagged) - Mouse hexosaminidase B (Hexb) |
CNY 7,220.00 |
