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Cplx3 (NM_146223) Mouse Tagged ORF Clone
CAT＃: MR201191
- TrueORF®
Cplx3 (Myc-DDK-tagged) - Mouse complexin 3 (Cplx3)
ORF Plasmid: tGFP
"NM_146223" in other vectors (5)
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CNY 1,200.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BC016632; CpxIII; ERGIC-53L; ERGL; Lamn1l; Lman1l |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR201191 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTTCATGGTGAAGTCCATGGTGGGTGGCCAACTGAAGAACCTCACAGGGAGCCTGGGGGGCGGCG AGGACAAGGGGGACGGAGACAAGTCTGCAGCCGAAGCACAGGGCATGAGCCGAGAAGAGTACGAGGAGTA TCAGAAGCAACTGGTGGAGGAGAAGATGGAGCGAGATGCACAGTTCACACAGAGGAAGGCAGAGCGGGCC ACACTGCGAAGTCACTTCAGAGACAAATACCGTCTGCCCAAGAACGAAACAGATGAAAGCCAGATCCAGC TGGCAGGCGGAGATGTGGAGCTGCCTCGGGAGCTAGCCAAGATGATCGAGGAGGATACGGAGGAAGAGGA GGATAAGGCCTCTGTGCTCGGGCAGCTGGCCAGCCTCCCTGGCCTAGACCTCAGCTCACTCAAGGACAAA GCCCAGACTACACTAGGGGACCTCAAGCAATCAGCTGAGAAGTGCCATATCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201191 protein sequence
Red=Cloning site Green=Tags(s) MAFMVKSMVGGQLKNLTGSLGGGEDKGDGDKSAAEAQGMSREEYEEYQKQLVEEKMERDAQFTQRKAERA TLRSHFRDKYRLPKNETDESQIQLAGGDVELPRELAKMIEEDTEEEEDKASVLGQLASLPGLDLSSLKDK AQTTLGDLKQSAEKCHIM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_146223 |
| ORF Size | 477 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_146223.3 |
| RefSeq Size | 2859 bp |
| RefSeq ORF | 477 bp |
| Locus ID | 235415 |
| UniProt ID | Q8R1B5 |
| MW | 17.6 kDa |
| Gene Summary | Complexin that regulates SNARE protein complex-mediated synaptic vesicle fusion (PubMed:19386896). Required for the maintenance of synaptic ultrastructure in the adult retina (PubMed:19386896). Positively regulates synaptic transmission through synaptic vesicle availability and exocytosis of neurotransmitters at photoreceptor ribbon synapses in the retina (PubMed:15911881, PubMed:19386896, PubMed:27335398). Suppresses tonic photoreceptor activity and baseline 'noise' by suppression of Ca(2+) vesicle tonic release and the facilitation of evoked synchronous and asynchronous Ca(2+) vesicle release (PubMed:22694764, PubMed:27335398).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201944 | Cplx3 (untagged) - Mouse complexin 3 (Cplx3), (10ug) |
CNY 1,200.00 |
|
| MG201191 | Cplx3 (tGFP-tagged) - Mouse complexin 3 (Cplx3) |
CNY 2,850.00 |
|
| MR201191L3 | Lenti ORF clone of Cplx3 (Myc-DDK-tagged) - Mouse complexin 3 (Cplx3) |
CNY 4,750.00 |
|
| MR201191L4 | Lenti ORF clone of Cplx3 (mGFP-tagged) - Mouse complexin 3 (Cplx3) |
CNY 4,750.00 |
|
| MR201193 | Cplx3 (Myc-DDK-tagged) - Mouse complexin 3 (Cplx3) |
CNY 1,200.00 |
