United States
Germany
Japan
United Kingdom
China
Cnot7 (NM_011135) Mouse Tagged ORF Clone
CAT＃: MR203889
- TrueORF®
Cnot7 (Myc-DDK-tagged) - Mouse CCR4-NOT transcription complex, subunit 7 (Cnot7)
ORF Plasmid: tGFP
"NM_011135" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 1,416.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AU022737; CAF-1; Caf1; Pop2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203889 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCAGCAACCGTAGATCATAGCCAAAGAATTTGTGAAGTTTGGGCTTGTAACCTGGATGAAGAGA TGAAGAAAATCCGTCAAGTTATCCGAAAATATAATTATGTTGCTATGGACACCGAGTTTCCAGGCGTTGT TGCAAGACCCATTGGAGAATTCAGAAGCAATGCTGACTATCAGTACCAACTGTTGCGGTGTAATGTAGAC TTGTTAAAGATAATCCAGCTCGGACTGACCTTTATGAATGAACAGGGAGAATACCCTCCAGGAACGTCAA CTTGGCAGTTTAACTTTAAGTTTAATTTGACGGAGGACATGTATGCTCAGGACTCTATAGAGCTACTAAC AACATCTGGTATCCAGTTTAAAAAACACGAGGAGGAAGGAATTGAGACCCAATATTTTGCAGAACTTCTT ATGACTTCAGGAGTGGTTCTTTGTGAAGGGGTCAAATGGCTATCATTTCACAGTGGTTATGACTTTGGCT ATTTAATCAAAATTCTGACCAACTCTAACTTGCCTGAGGAAGAACTTGATTTCTTTGAGATCCTTCGGTT ATTTTTTCCTGTCATTTATGATGTGAAGTACCTCATGAAGAGCTGCAAAAATCTCAAAGGTGGATTACAG GAAGTTGCTGAGCAGTTAGAGCTGGAGCGCATAGGCCCTCAGCACCAGGCAGGATCTGACTCACTGCTTA CAGGAATGGCCTTTTTCAAAATGAGAGAAATGTTCTTTGAAGATCACATTGATGATGCCAAATACTGTGG TCACTTATATGGCCTTGGTTCTGGCTCATCCTATGTACAGAACGGCACAGGGAATGCATATGAAGAGGAA GCCAGCAAGCAGTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203889 protein sequence
Red=Cloning site Green=Tags(s) MPAATVDHSQRICEVWACNLDEEMKKIRQVIRKYNYVAMDTEFPGVVARPIGEFRSNADYQYQLLRCNVD LLKIIQLGLTFMNEQGEYPPGTSTWQFNFKFNLTEDMYAQDSIELLTTSGIQFKKHEEEGIETQYFAELL MTSGVVLCEGVKWLSFHSGYDFGYLIKILTNSNLPEEELDFFEILRLFFPVIYDVKYLMKSCKNLKGGLQ EVAEQLELERIGPQHQAGSDSLLTGMAFFKMREMFFEDHIDDAKYCGHLYGLGSGSSYVQNGTGNAYEEE ASKQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011135 |
| ORF Size | 858 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011135.5 |
| RefSeq Size | 2617 bp |
| RefSeq ORF | 858 bp |
| Locus ID | 18983 |
| UniProt ID | Q60809 |
| MW | 32.7 kDa |
| Gene Summary | Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT8. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Required for miRNA-mediated mRNA deadenylation. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200486 | Cnot7 (untagged) - Mouse CCR4-NOT transcription complex, subunit 7 (Cnot7), (10ug) |
CNY 2,000.00 |
|
| MG203889 | Cnot7 (tGFP-tagged) - Mouse CCR4-NOT transcription complex, subunit 7 (Cnot7) |
CNY 2,850.00 |
|
| MR203889L3 | Lenti ORF clone of Cnot7 (Myc-DDK-tagged) - Mouse CCR4-NOT transcription complex, subunit 7 (Cnot7) |
CNY 4,750.00 |
|
| MR203889L4 | Lenti ORF clone of Cnot7 (mGFP-tagged) - Mouse CCR4-NOT transcription complex, subunit 7 (Cnot7) |
CNY 4,750.00 |
