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Krt19 (NM_008471) Mouse Tagged ORF Clone
CAT＃: MR206318
- TrueORF®
Krt19 (Myc-DDK-tagged) - Mouse keratin 19 (Krt19)
ORF Plasmid: tGFP
"NM_008471" in other vectors (4)
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CNY 2,850.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI663979; CK-19; End; EndoC; K19; Krt-1.1; Krt-1.19; Krt1-1; Krt1-19 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206318 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTTCCTATAGCTATCGCCAGACCTCAGCTATGTCTTCCTTTGGGGGTACGGGCGGGGGTTCAGTAC GCATTGGGTCAGGGGGTGTTTTCCGCGCACCCAGCATCCACGGGGGCTCAGGTGGCCGCGGCGTGTCCGT GTCCTCCACCCGCTTCGTGACCTCGTCCTCCGGGAGCTATGGCGGAGTCCGCGGTGGAAGTTTTAGTGGG ACCCTGGCTGTGTCTGATGGGCTGCTGTCTGGCAATGAGAAGATCACCATGCAAAACCTCAATGATCGTC TCGCCTCCTACTTGGACAAGGTGCGCGCCCTAGAGCAGGCCAATGGCGAGCTGGAGGTGAAGATCCGCGA CTGGTACCAGAAGCAGGGACCCGGACCCTCCCGAGATTACAACCACTACTTTAAGACCATCGAGGACTTG CGCGACAAGATTCTTGGTGCCACCATTGACAACTCCAAGATTGTCCTACAGATTGACAATGCTCGCCTGG CTGCAGATGACTTCAGAACCAAGTTTGAGACAGAACACGCCTTGCGTCTGAGCGTGGAGGCCGACATCAA CGGCCTGCGCCGGGTGCTGGATGAGCTGACTCTGGCCAGGACTGACCTGGAGATGCAGATTGAGAGCCTG AAGGAGGAGCTGGCCTACCTGAAGAAGAACCATGAGGAGGAAATTACTGCCCTGAGGAGCCAGGTGGGTG GCCAGGTCAGTGTGGAGGTGGATTCCACTCCCGGTGTCGACCTAGCCAAGATCCTGAGTGAGATGAGAAG TCAGTATGAGATCATGGCCGAGAAGAACCGGAAGGATGCTGAAGCCACCTACCTTGCTCGGATTGAGGAG CTGAACACCCAGGTCGCCGTCCACTCTGAGCAGATCCAGATAAGCAAGACCGAAGTCACGGACCTTCGAC GGACCCTCCAGGGCCTTGAGATTGAGCTGCAGTCCCAGCTCAGCATGAAAGCTGCCCTGGAAGGCACGCT GGCAGAGACGGAGGCCCGTTATGGAGTCCAGCTGTCACAGATCCAGAGCGTGATCAGCGGTTTTGAAGCC CAGCTGAGCGACGTGCGTGCCGACATAGAGCGCCAGAACCAGGAGTATAAGCAGCTCATGGACATCAAGT CCAGGCTGGAGCAGGAGATCGCCACCTACCGCAGCCTGCTGGAGGGCCAGGAAGCCCACTACAACAATCT GCCCACCCCCAAGGCCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206318 protein sequence
Red=Cloning site Green=Tags(s) MTSYSYRQTSAMSSFGGTGGGSVRIGSGGVFRAPSIHGGSGGRGVSVSSTRFVTSSSGSYGGVRGGSFSG TLAVSDGLLSGNEKITMQNLNDRLASYLDKVRALEQANGELEVKIRDWYQKQGPGPSRDYNHYFKTIEDL RDKILGATIDNSKIVLQIDNARLAADDFRTKFETEHALRLSVEADINGLRRVLDELTLARTDLEMQIESL KEELAYLKKNHEEEITALRSQVGGQVSVEVDSTPGVDLAKILSEMRSQYEIMAEKNRKDAEATYLARIEE LNTQVAVHSEQIQISKTEVTDLRRTLQGLEIELQSQLSMKAALEGTLAETEARYGVQLSQIQSVISGFEA QLSDVRADIERQNQEYKQLMDIKSRLEQEIATYRSLLEGQEAHYNNLPTPKAI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008471 |
| ORF Size | 1212 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008471.3 |
| RefSeq Size | 1509 bp |
| RefSeq ORF | 1212 bp |
| Locus ID | 16669 |
| UniProt ID | P19001 |
| MW | 44.5 kDa |
| Gene Summary | The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203141 | Krt19 (untagged) - Mouse keratin 19 (Krt19), (10ug) |
CNY 2,000.00 |
|
| MG206318 | Krt19 (tGFP-tagged) - Mouse keratin 19 (Krt19) |
CNY 3,140.00 |
|
| MR206318L3 | Lenti ORF clone of Krt19 (Myc-DDK-tagged) - Mouse keratin 19 (Krt19) |
CNY 4,750.00 |
|
| MR206318L4 | Lenti ORF clone of Krt19 (mGFP-tagged) - Mouse keratin 19 (Krt19) |
CNY 4,750.00 |
