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Atp6ap1 (NM_018794) Mouse Tagged ORF Clone
CAT＃: MR207392
- TrueORF®
Atp6ap1 (Myc-DDK-tagged) - Mouse ATPase, H+ transporting, lysosomal accessory protein 1 (Atp6ap1)
"NM_018794" in other vectors (3)
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CNY 3,656.00
CNY 4,090.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 16A; AC45; AI316502; Atp6ip1; Atp6s1; AW108110; C7-1; CF2; mFLJ00383; VATPS1; XAP-3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207392 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGCGGCAACAGTGGTATCTCGGATACGGACGGGGACAGGGCGGGCTCCAGTCATGTGGCTGTCTC TGTCGTTGGTGGCAGTGGCAGCAGCAGTGGCCACGGAGCAGCAGGTGCCACTGGTACTGTGGTCGAGTGA CCGGAATCTGTGGGCTCCTGTGGCCGACACACATGAGGGTCATATCACCAGCGATATGCAGCTTTCTACC TACTTAGACCCTGCCCTGGAGCTGGGTCCCCGTAATGTACTGCTGTTCCTACAGGACAAGCTAAGCATTG AGGATTTCACAGCATACGGTGGTGTGTTTGGAAATAAGCAGGACAGTGCCTTTTCTAACCTGGAGAATGC CCTGGACTTGGCCCCCTCCTCACTGGTGCTTCCTGCTGTGGACTGGTATGCAATCAGCACTCTGACCACC TACCTACAGGAGAAGCTTGGGGCTAGCCCCTTGCATGTGGATCTAGCTACCTTAAAGGAGCTGAAGCTCA ATGCCAGCCTTCCTGCCCTGCTGCTCATCCGTCTGCCCTACACAGCCAGCTCCGGTCTGATGGCGCCCAG GGAGGTCCTCACAGGTAACGATGAGGTCATCGGACAGGTACTGAGCACACTCAAGTCTGAAGATGTCCCT TACACCGCAGCTCTTACTGCAGTCCGCCCTTCTAGAGTGGCCCGTGATATAACCATGGTGGCTGGGGGTC TAGGTCGCCAGCTGCTTCAGACTCAGGTGGCATCTCCTGCGATCCATCCTCCTGTGAGTTACAATGACAC TGCCCCAAGGATCCTGTTTTGGGCACAAAACTTCTCTGTGGCATACAAAGACGAGTGGAAAGACTTGACC TCCCTCACCTTTGGTGTGGAGAATCTTAACTTGACCGGCTCCTTCTGGAATGACTCCTTTGCCATGCTTT CACTGACCTATGAGCCACTCTTTGGTGCAACAGTGACATTCAAGTTCATTCTGGCTAGTCGCTTCTATCC AGTGTCTGCCCGATATTGGTTTGCCATGGAACGACTTGAAATCCACAGCAATGGCTCTGTTGCCCATTTC AATGTTTCCCAAGTCACAGGACCTAGCATCTATTCTTTCCACTGTGAGTACGTCAGCAGTGTGAGCAAGA AAGGCAATCTCCTTGTGACCAACGTGCCCTCAGTCTGGCAGATGACTCTTCATAACTTCCAGATCCAGGC CTTCAATGTGACTGGTGAACAGTTCTCCTATGCTAGCGACTGTGCTGGTTTCTTCTCTCCGGGCATCTGG ATGGGTCTGCTCACCACCCTCTTCATGCTTTTCATATTCACCTATGGTCTGCACATGATACTCAGCCTAA AGACCATGGATCGCTTTGATGACCACAAGGGCCCCACCATCACTTTGACCCAGATTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207392 protein sequence
Red=Cloning site Green=Tags(s) MMAATVVSRIRTGTGRAPVMWLSLSLVAVAAAVATEQQVPLVLWSSDRNLWAPVADTHEGHITSDMQLST YLDPALELGPRNVLLFLQDKLSIEDFTAYGGVFGNKQDSAFSNLENALDLAPSSLVLPAVDWYAISTLTT YLQEKLGASPLHVDLATLKELKLNASLPALLLIRLPYTASSGLMAPREVLTGNDEVIGQVLSTLKSEDVP YTAALTAVRPSRVARDITMVAGGLGRQLLQTQVASPAIHPPVSYNDTAPRILFWAQNFSVAYKDEWKDLT SLTFGVENLNLTGSFWNDSFAMLSLTYEPLFGATVTFKFILASRFYPVSARYWFAMERLEIHSNGSVAHF NVSQVTGPSIYSFHCEYVSSVSKKGNLLVTNVPSVWQMTLHNFQIQAFNVTGEQFSYASDCAGFFSPGIW MGLLTTLFMLFIFTYGLHMILSLKTMDRFDDHKGPTITLTQIV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018794 |
| ORF Size | 1392 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018794.4, NP_061264.1 |
| RefSeq Size | 2189 bp |
| RefSeq ORF | 1392 bp |
| Locus ID | 54411 |
| UniProt ID | Q9R1Q9 |
| MW | 51 kDa |
| Gene Summary | Accessory subunit of the proton-transporting vacuolar (V)-ATPase protein pump, which is required for luminal acidification of secretory vesicles. Guides the V-type ATPase into specialized subcellular compartments, such as neuroendocrine regulated secretory vesicles or the ruffled border of the osteoclast, thereby regulating its activity. Involved in membrane trafficking and Ca(2+)-dependent membrane fusion. May play a role in the assembly of the V-type ATPase complex. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205454 | Atp6ap1 (untagged) - Mouse ATPase, H+ transporting, lysosomal accessory protein 1 (Atp6ap1), (10ug) |
CNY 2,000.00 |
|
| MR207392L3 | Lenti ORF clone of Atp6ap1 (Myc-DDK-tagged) - Mouse ATPase, H+ transporting, lysosomal accessory protein 1 (Atp6ap1) |
CNY 5,990.00 |
|
| MR207392L4 | Lenti ORF clone of Atp6ap1 (mGFP-tagged) - Mouse ATPase, H+ transporting, lysosomal accessory protein 1 (Atp6ap1) |
CNY 5,990.00 |
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