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Gdf5 (NM_008109) Mouse Tagged ORF Clone
CAT＃: MR207949
- TrueORF®
Gdf5 (Myc-DDK-tagged) - Mouse growth differentiation factor 5 (Gdf5)
ORF Plasmid: tGFP
"NM_008109" in other vectors (4)
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CNY 3,656.00
CNY 4,370.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | b; BMP-14; bp; brp; CDMP-; Cdmp-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207949 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGACTCCCCAAACTCCTCACTCTTTTGCTGTGGCACCTGGCTTGGCTGGACCTGGAACTCATCTGCA CTGTGCTGGGTGCCCCTGACTTAGGACAGAGAACCCCAGGGGCCAAGCCAGGGTTGACCAAAGCGGAGGC CAAGGAGAGGCCACCCCTGGCCAGGAATGTCTTTAGGCCAGGGGGTCATATCTATGGTGTGGGGGCCACC AATGCCAGGGCCAAGGGAAGCTCTGGGCAGACACAGGCCAAGAAGGATGAACCCAGAAAGATGCCCCCCA GATCCGGTGGCTCTGAAACCAAGCCAGGACCCTCTTCCCAGACTAGACAGGCTGCAGCCCGGACTGTAAC CCCAAAAGGACAGCTTCCTGGGGGCAAAGCATCTTCAAAAGCAGGATCTGCCCCCAGCTCCTTCCTGCTG AAGAAGACCAGGGAGCCTGGGACCCCTCGAGAGCCCAAGGAGCCGTTCCGCCCGCCCCCCATCACACCCC ACGAATACATGCTCTCCCTGTACAGGACGCTGTCCGATGCTGACAGAAAGGGAGGTAACAGCAGCGTGAA GTTGGAGGCTGGCCTGGCCAACACCATCACCAGCTTTATTGACAAAGGGCAAGATGACCGAGGCCCTGCG GTCAGGAAGCAGAGGTACGTGTTTGACATCAGTGCCTTGGAGAAGGATGGGCTGTTGGGGGCTGAACTGC GGATCTTACGGAAGAAGCCCTTGGACGTGGCCAAGCCAGCGGTCCCCAGTAGCGGGCGGGTTGCCCAACT GAAGCTGTCCAGCTGCCCCAGCGGCCGGCAGCCGGCAGCCTTGCTGGATGTGCGCTCCGTGCCAGGCCTG GATGGATCTGGCTGGGAGGTGTTCGACATCTGGAAGCTCTTCCGAAATTTTAAGAACTCAGCGCAGCTGT GCCTGGAGCTGGAGGCCTGGGAACGGGGCCGGGCCGTGGACCTCCGTGGCCTGGGCTTTGAACGCACTGC CCGACAGGTCCACGAGAAAGCCTTGTTCCTAGTGTTTGGTCGTACCAAGAAACGGGACCTGTTCTTTAAT GAGATTAAGGCCCGCTCTGGCCAGGATGACAAGACTGTGTATGAATATTTGTTCAGCCAGCGGCGGAAAC GCCGGGCCCCATTGGCCAATCGCCAGGGCAAGCGACCCAGCAAGAACCTCAAGGCTCGCTGCAGTCGCAA GGCCTTGCATGTCAACTTCAAGGACATGGGCTGGGACGACTGGATCATCGCACCTCTTGAGTATGAGGCC TTCCACTGCGAAGGACTGTGTGAGTTCCCCTTGCGCTCCCACTTGGAGCCCACAAACCACGCAGTCATTC AGACCCTAATGAACTCTATGGACCCTGAATCCACACCACCCACTTGTTGTGTGCCTACACGGCTGAGTCC TATTAGCATCCTCTTCATCGACTCTGCCAACAACGTGGTGTATAAACAGTACGAGGACATGGTCGTGGAA TCTTGTGGCTGCAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207949 protein sequence
Red=Cloning site Green=Tags(s) MRLPKLLTLLLWHLAWLDLELICTVLGAPDLGQRTPGAKPGLTKAEAKERPPLARNVFRPGGHIYGVGAT NARAKGSSGQTQAKKDEPRKMPPRSGGSETKPGPSSQTRQAAARTVTPKGQLPGGKASSKAGSAPSSFLL KKTREPGTPREPKEPFRPPPITPHEYMLSLYRTLSDADRKGGNSSVKLEAGLANTITSFIDKGQDDRGPA VRKQRYVFDISALEKDGLLGAELRILRKKPLDVAKPAVPSSGRVAQLKLSSCPSGRQPAALLDVRSVPGL DGSGWEVFDIWKLFRNFKNSAQLCLELEAWERGRAVDLRGLGFERTARQVHEKALFLVFGRTKKRDLFFN EIKARSGQDDKTVYEYLFSQRRKRRAPLANRQGKRPSKNLKARCSRKALHVNFKDMGWDDWIIAPLEYEA FHCEGLCEFPLRSHLEPTNHAVIQTLMNSMDPESTPPTCCVPTRLSPISILFIDSANNVVYKQYEDMVVE SCGCR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008109 |
| ORF Size | 1488 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008109.1, NP_032135.1 |
| RefSeq Size | 2317 bp |
| RefSeq ORF | 1488 bp |
| Locus ID | 14563 |
| UniProt ID | P43027 |
| MW | 54.9 kDa |
| Gene Summary | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates the development of numerous tissue and cell types, including cartilage, joints, brown fat, teeth, and the growth of neuronal axons and dendrites. Mice with a mutation in this gene exhibit enhanced tooth enamel formation. [provided by RefSeq, Aug 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216933 | Gdf5 (untagged) - Mouse growth differentiation factor 5 (Gdf5), (10ug) |
CNY 3,656.00 |
|
| MG207949 | Gdf5 (tGFP-tagged) - Mouse growth differentiation factor 5 (Gdf5) |
CNY 4,850.00 |
|
| MR207949L3 | Lenti ORF clone of Gdf5 (Myc-DDK-tagged) - Mouse growth differentiation factor 5 (Gdf5) |
CNY 6,270.00 |
|
| MR207949L4 | Lenti ORF clone of Gdf5 (mGFP-tagged) - Mouse growth differentiation factor 5 (Gdf5) |
CNY 6,270.00 |
