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Sh2b3 (NM_008507) Mouse Tagged ORF Clone
CAT＃: MR208726
- TrueORF®
Sh2b3 (Myc-DDK-tagged) - Mouse SH2B adaptor protein 3 (Sh2b3)
ORF Plasmid: tGFP
"NM_008507" in other vectors (4)
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CNY 4,080.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI429800; L; Lnk |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208726 representing NM_008507
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACGAGCCCACCGTGCAGCCGTCCCGCACATCCTCCGCACCCGCCTCGCCGGCATCCCCACGCGGCT GGAGCGACTTCTGCGAGCAGCACGCAGCAGCGGCGGCCCGGGAGCTGGCCCGCCAGTACTGGTTGTTTGC GCGCGCGCACCCACAGCCGCCGCGCGCGGACCTGGTGTCGCTGCAGTTCGCGGAGCTCTTCCAGCGCCAC TTCTGCCGGGAGGTGCGCGAGAGCCTCGCAGGACCGCCGGGTCACGACTACCGCGCCACTGCTCCGCCCC GCCCCGCGCTGCCCAAGGCACGCAGCTCCGAGGACCTGGGCCCGCGGCCCGCCTGTGCCCTGCAGCACCT GCGCCGCGGCCTGCGCCAGCTCTTCCGCCGCCGCTCGGCAGGGGAGCTGCCCGGGGCTACCAGTGACACC AATGACATCGACACCACCGCAGCCAGCAGGCCGGGCCCGGCCCGCAAGTTGCTACCCTGGGGCCTGCGAG AGCCGCCCACTGAGGCGCTCAAGGAGGTCGTATTGCGCTATAGCCTGGCGGACGAGGCAGCAATGGACAG CGGCGCACGCTGGCAGCGGGGTCGCCTGGTGCTTCGGTCTCCAGGTCCGGGCCACAGCCACTTTCTGCAG CTCTTCGATCCGCCCAAGAGCTCAAAGCCCAAGCTCCAAGAGGCCTGTTCCAGCATCCGGGAGGTCCGAC CATGTACACGCCTGGAGATGCCTGACAACCTCTACACCTTTGTGTTGAAGGTGCAGGACCAGACAGACAT CATCTTTGAGGTGGGAGATGAACAGCAGCTGAACTCATGGCTGGCAGAGCTCAGGGCAAGCACAGGCCTT GGGCTGGAGCACCCGGACACCGAGTTACCTCTTTCCTTAGCGGCAGAGCCTGGCCCAGCTAGATCCCCAA GGGGAAGCACTGACTCCCTGGACCAAGGTGCTTCACCTGGGGTGTTGCTGGACCCAGCCTGCCAGAAAAC AGATCACTTCCTATCCTGCTACCCCTGGTTCCACGGCCCCATCTCCAGGGTGAGGGCTGCACAGCTGGTC CAGCTCCAGGGCCCTGATGCCCACGGCGTGTTCCTGGTGCGGCAGAGTGAGTCCCGGAGAGGAGAGTATG TACTCACATTCAACTTACAGGGCAGAGCCAAGCACTTACGCCTGGTGCTCACAGAGCGTGGACAGTGCCG GGTGCAACACCTGCACTTCCCCTCGGTGGTAGATATGCTCCGCCACTTCCAGCGTTCTCCTATCCCACTG GAATGTGGAGCAGCTTGTGACGTCCGACTCTCTGGCTATGTGGTAGTCCTCTCTCAGGCACCAGGTTCCT CCAACACCGTCCTCTTCCCTTTTTCCCTTCCTCACTGGGATTCGGAGCTGGGTCATCCCCACCTCAGCTC TGTTGGCTGTCCCCCCAGCCATGGTGCAGAGGCTCTCCCTGGCCAAGTGACACCACCTGAGCAGATCTTC CACCTGGTGCCTTCTCCTGAGGAACTGGCCAACAGTCTGCGGCAGCTGGAGCTCGAGTCTGTGAGCAGTG CCCGGGACTCGGACTATGACATGGACTCCTCTTCACGGGGCCACCTTCGGGCCATTGACAACCAGTACAC CCCTCTCTCACAGCTGTGCAGAGAGGCAGACGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208726 representing NM_008507
Red=Cloning site Green=Tags(s) MNEPTVQPSRTSSAPASPASPRGWSDFCEQHAAAAARELARQYWLFARAHPQPPRADLVSLQFAELFQRH FCREVRESLAGPPGHDYRATAPPRPALPKARSSEDLGPRPACALQHLRRGLRQLFRRRSAGELPGATSDT NDIDTTAASRPGPARKLLPWGLREPPTEALKEVVLRYSLADEAAMDSGARWQRGRLVLRSPGPGHSHFLQ LFDPPKSSKPKLQEACSSIREVRPCTRLEMPDNLYTFVLKVQDQTDIIFEVGDEQQLNSWLAELRASTGL GLEHPDTELPLSLAAEPGPARSPRGSTDSLDQGASPGVLLDPACQKTDHFLSCYPWFHGPISRVRAAQLV QLQGPDAHGVFLVRQSESRRGEYVLTFNLQGRAKHLRLVLTERGQCRVQHLHFPSVVDMLRHFQRSPIPL ECGAACDVRLSGYVVVLSQAPGSSNTVLFPFSLPHWDSELGHPHLSSVGCPPSHGAEALPGQVTPPEQIF HLVPSPEELANSLRQLELESVSSARDSDYDMDSSSRGHLRAIDNQYTPLSQLCREADV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008507 |
| ORF Size | 1644 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008507.4 |
| RefSeq Size | 2498 bp |
| RefSeq ORF | 1647 bp |
| Locus ID | 16923 |
| UniProt ID | O09039 |
| MW | 60.9 kDa |
| Gene Summary | This gene encodes a member of the SH2B family of adapter proteins that play an important role in T cell receptor signaling. This gene is preferentially expressed in hematopoietic stem cells, hematopoietic progenitors, pre and immature B cells, as well as megakaryocytes and mastocytes. In hematopoietic stem cells, the encoded protein is a key regulator of self-renewal, proliferation and apoptosis. Mice lacking the encoded protein exhibit pre and immature B cell expansion in spleen and the bone marrow. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203727 | Sh2b3 (untagged) - Mouse SH2B adaptor protein 3 (Sh2b3), (10ug) |
CNY 2,000.00 |
|
| MG208726 | Sh2b3 (tGFP-tagged) - Mouse SH2B adaptor protein 3 (Sh2b3) |
CNY 5,040.00 |
|
| MR208726L3 | Lenti ORF clone of Sh2b3 (Myc-DDK-tagged) - Mouse SH2B adaptor protein 3 (Sh2b3) |
CNY 6,460.00 |
|
| MR208726L4 | Lenti ORF clone of Sh2b3 (mGFP-tagged) - Mouse SH2B adaptor protein 3 (Sh2b3) |
CNY 6,460.00 |
