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Gpc1 (NM_016696) Mouse Tagged ORF Clone
CAT＃: MR208830
- TrueORF®
Gpc1 (Myc-DDK-tagged) - Mouse glypican 1 (Gpc1)
ORF Plasmid: tGFP
"NM_016696" in other vectors (4)
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CNY 4,152.00
CNY 4,940.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI462976 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208830 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACTCCGGACCCGAGGCTGGTGGCTGCTGTGCGCGGCCGCCGCGCTGGTCGTCTGCGCCCGCGGGG ACCCCGCCAGCAAGAGCCGGAGCTGCAGCGAAGTCCGCCAGATCTACGGGGCTAAGGGCTTTAGCCTGAG CGATGTGCCTCAGGCAGAGATCTCGGGTGAGCACCTGCGGATCTGCCCCCAGGGCTACACTTGCTGTACT AGTGAGATGGAGGAGAATTTGGCCAACCACAGCCGAATGGAGCTGGAGAGCGCACTCCATGACAGCAGCC GCGCCCTGCAGGCCACACTGGCCACCCAGCTGCATGGCATCGATGACCACTTCCAGCGCCTGCTGAATGA CTCGGAGCGCACACTGCAGGAGGCTTTCCCTGGGGCCTTTGGGGACCTGTATACGCAGAACACTCGTGCC TTCCGGGACCTATATGCTGAGCTGCGCCTCTACTACCGTGGGGCCAACCTGCACCTTGAGGAGACGCTGG CCGAGTTCTGGGCACGGCTGCTGGAGCGCCTCTTCAAGCAGCTGCACCCCCAGCTGCTGCCTGATGACTA CCTGGACTGCCTGGGCAAGCAGGCGGAGGCACTGCGGCCGTTTGGAGATGCCCCTCGAGAACTGCGCCTG CGGGCCACCCGTGCCTTTGTGGCTGCACGTTCCTTTGTGCAGGGCCTGGGTGTGGCCAGTGATGTAGTCC GGAAGGTGGCCCAGGTACCTCTGGCCCCAGAATGTTCTCGGGCCATCATGAAGTTGGTCTACTGTGCTCA TTGCCGGGGAGTCCCGGGCGCCCGGCCCTGCCCCGACTATTGCCGAAATGTGCTCAAAGGCTGCCTTGCC AACCAGGCCGACCTGGATGCCGAGTGGAGGAACCTCCTGGACTCCATGGTGCTCATCACTGACAAGTTCT GGGGCCCGTCGGGTGCGGAGAGTGTCATTGGCGGTGTGCACGTGTGGCTGGCGGAGGCCATCAACGCCCT CCAGGACAACAAGGACACACTCACAGCTAAGGTCATCCAGGCCTGTGGAAACCCCAAGGTCAATCCCCAC GGCTCTGGGCCCGAGGAGAAGCGTCGCCGTGGCAAATTGGCACTGCAGGAGAAGCCCTCCACAGGTACTC TGGAAAAACTGGTCTCTGAGGCCAAGGCCCAGCTCCGAGACATTCAGGACTTCTGGATCAGCCTCCCAGG GACACTGTGCAGTGAGAAGATGGCCATGAGTCCTGCCAGTGACGACCGCTGCTGGAATGGAATTTCCAAG GGCCGGTACCTACCAGAGGTGATGGGTGACGGGCTGGCCAACCAGATCAACAACCCTGAGGTGGAAGTGG ACATCACCAAGCCAGACATGACCATCCGCCAGCAGATTATGCAGCTCAAGATCATGACCAACCGTTTACG TGGCGCCTATGGCGGCAACGACGTGGACTTCCAGGATGCTAGTGATGACGGCAGTGGCTCCGGCAGCGGT GGCGGATGCCCAGATGACACCTGTGGCCGGAGGGTCAGCAAGAAGAGTTCCAGCTCCCGGACCCCCTTGA CCCATGCCCTCCCCGGCCTGTCAGAACAGGAGGGACAGAAGACCTCAGCTGCCACCTGCCCAGAGCCCCA CAGCTTCTTCCTGCTCTTCCTCGTCACCTTGGTCCTTGCGGCAGCCAGGCCCAGGTGGCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208830 protein sequence
Red=Cloning site Green=Tags(s) MELRTRGWWLLCAAAALVVCARGDPASKSRSCSEVRQIYGAKGFSLSDVPQAEISGEHLRICPQGYTCCT SEMEENLANHSRMELESALHDSSRALQATLATQLHGIDDHFQRLLNDSERTLQEAFPGAFGDLYTQNTRA FRDLYAELRLYYRGANLHLEETLAEFWARLLERLFKQLHPQLLPDDYLDCLGKQAEALRPFGDAPRELRL RATRAFVAARSFVQGLGVASDVVRKVAQVPLAPECSRAIMKLVYCAHCRGVPGARPCPDYCRNVLKGCLA NQADLDAEWRNLLDSMVLITDKFWGPSGAESVIGGVHVWLAEAINALQDNKDTLTAKVIQACGNPKVNPH GSGPEEKRRRGKLALQEKPSTGTLEKLVSEAKAQLRDIQDFWISLPGTLCSEKMAMSPASDDRCWNGISK GRYLPEVMGDGLANQINNPEVEVDITKPDMTIRQQIMQLKIMTNRLRGAYGGNDVDFQDASDDGSGSGSG GGCPDDTCGRRVSKKSSSSRTPLTHALPGLSEQEGQKTSAATCPEPHSFFLLFLVTLVLAAARPRWR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016696 |
| ORF Size | 1674 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016696.5 |
| RefSeq Size | 3552 bp |
| RefSeq ORF | 1674 bp |
| Locus ID | 14733 |
| UniProt ID | Q9QZF2 |
| MW | 61.4 kDa |
| Gene Summary | Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination (By similarity). May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling. Binds Cu(2+) or Zn(2+) ions.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202606 | Gpc1 (untagged) - Mouse glypican 1 (Gpc1), (10ug) |
CNY 2,000.00 |
|
| MG208830 | Gpc1 (tGFP-tagged) - Mouse glypican 1 (Gpc1) |
CNY 5,752.00 |
|
| MR208830L3 | Lenti ORF clone of Gpc1 (Myc-DDK-tagged) - Mouse glypican 1 (Gpc1) |
CNY 6,552.00 |
|
| MR208830L4 | Lenti ORF clone of Gpc1 (mGFP-tagged) - Mouse glypican 1 (Gpc1) |
CNY 6,552.00 |
