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Egf (NM_010113) Mouse Tagged ORF Clone
CAT＃: MR211815
- TrueORF®
Egf (Myc-DDK-tagged) - Mouse epidermal growth factor (Egf)
ORF Plasmid: tGFP
"NM_010113" in other vectors (4)
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CNY 12,720.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI790464 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211815 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTGGGGCCGAAGGCCAACCTGGCTGTTGCTCGCCTTCCTGCTGGTGTTTTTAAAGATTAGCATAC TCAGCGTCACAGCATGGCAGACCGGGAACTGTCAGCCAGGTCCTCTCGAGAGAAGCGAGAGAAGCGGGAC TTGTGCCGGTCCTGCCCCCTTCCTAGTTTTCTCACAAGGAAAGAGCATCTCTCGGATTGACCCAGATGGA ACAAATCACCAGCAATTGGTGGTGGATGCTGGCATCTCAGCAGACATGGATATTCATTATAAAAAAGAGA GACTCTATTGGGTGGATGTAGAAAGACAAGTTTTGCTAAGAGTTTTCCTTAACGGGACAGGACTAGAGAA AGTGTGCAATGTAGAGAGGAAGGTGTCTGGGCTGGCCATAGACTGGATAGATGATGAAGTTCTCTGGGTA GACCAACAGAACGGAGTCATCACCGTAACAGATATGACAGGGAAAAATTCCCGAGTTCTTCTAAGTTCCT TAAAACATCCGTCAAATATAGCAGTGGATCCAATAGAGAGGTTGATGTTTTGGTCTTCAGAGGTGACCGG CAGCCTTCACAGAGCACACCTCAAAGGTGTTGATGTAAAAACACTGCTGGAGACAGGGGGAATATCGGTG CTGACTCTGGATGTCCTGGACAAACGGCTCTTCTGGGTTCAGGACAGTGGCGAAGGAAGCCACGCTTACA TTCATTCCTGTGATTATGAGGGTGGCTCCGTCCGTCTTATCAGGCATCAAGCACGGCACAGTTTGTCTTC AATGGCCTTTTTTGGTGATCGGATCTTCTACTCAGTGTTGAAAAGCAAGGCGATTTGGATAGCCAACAAA CACACGGGGAAGGACACGGTCAGGATTAACCTCCATCCATCCTTTGTGACACCTGGAAAACTGATGGTAG TACACCCTCGTGCACAGCCCAGGACAGAGGACGCTGCTAAGGATCCTGACCCCGAACTTCTCAAACAGAG GGGAAGACCATGCCGCTTCGGTCTCTGTGAGCGAGACCCCAAGTCCCACTCGAGCGCATGCGCTGAGGGC TACACGTTAAGCCGAGACCGGAAGTACTGCGAAGATGTCAATGAATGTGCCACTCAGAATCACGGCTGTA CTCTTGGGTGTGAAAACACCCCTGGATCCTATCACTGCACATGCCCCACAGGATTTGTTCTGCTTCCTGA TGGGAAACAATGTCACGAACTTGTTTCCTGCCCAGGCAACGTATCAAAGTGCAGTCATGGCTGTGTCCTG ACATCAGATGGTCCCCGGTGCATCTGTCCTGCAGGTTCAGTGCTTGGGAGAGATGGGAAGACTTGCACTG GTTGTTCATCGCCTGACAATGGTGGATGCAGCCAGATCTGTCTTCCTCTCAGGCCAGGATCCTGGGAATG TGATTGCTTTCCTGGGTATGACCTACAGTCAGACCGAAAGAGCTGTGCAGCTTCAGGACCACAGCCACTT TTACTGTTTGCAAATTCCCAGGACATCCGACACATGCATTTTGATGGAACAGACTACAAAGTTCTGCTCA GCCGGCAGATGGGAATGGTTTTTGCCTTGGATTATGACCCTGTGGAAAGCAAGATATATTTTGCACAGAC AGCCCTGAAGTGGATAGAGAGGGCTAATATGGATGGGTCCCAGCGAGAAAGACTGATCACAGAAGGAGTA GATACGCTTGAAGGTCTTGCCCTGGACTGGATTGGCCGGAGAATCTACTGGACAGACAGTGGGAAGTCTG TTGTTGGAGGGAGCGATCTGAGCGGGAAGCATCATCGAATAATCATCCAGGAGAGAATCTCGAGGCCGCG AGGAATAGCTGTGCATCCAAGGGCCAGGAGACTGTTCTGGACGGACGTAGGGATGTCTCCACGGATTGAA AGCGCTTCCCTTCAAGGTTCCGACCGGGTGCTGATAGCCAGCTCCAATCTACTGGAACCCAGTGGAATCA CGATTGACTACTTAACAGACACTTTGTACTGGTGTGACACCAAGAGGTCTGTGATTGAAATGGCCAATCT GGATGGCTCCAAACGCCGAAGACTTATCCAGAACGACGTAGGTCACCCCTTCTCTCTAGCCGTGTTTGAG GATCACCTGTGGGTCTCGGATTGGGCTATCCCATCGGTAATAAGGGTGAACAAGAGGACTGGCCAAAACA GGGTACGTCTTCAAGGCAGCATGCTGAAGCCCTCGTCACTGGTTGTGGTCCATCCATTGGCAAAACCAGG TGCAGATCCCTGCTTATACAGGAATGGAGGCTGTGAACACATCTGCCAAGAGAGCCTGGGCACAGCTCGG TGTTTGTGTCGTGAAGGTTTTGTGAAGGCCTGGGATGGGAAAATGTGTCTCCCTCAGGATTATCCAATCC TGTCAGGTGAAAATGCTGATCTTAGTAAAGAGGTGACATCACTGAGCAACTCCACTCAGGCTGAAGTACC AGACGATGATGGGACAGAATCTTCCACACTAGTGGCTGAAATCATGGTGTCAGGCATGAACTATGAAGAT GACTGTGGTCCCGGGGGGTGTGGAAGCCATGCTCGATGCGTTTCAGACGGAGAGACTGCTGAGTGTCAGT GTCTGAAAGGGTTTGCCAGGGATGGAAACCTGTGTTCTGATATAGATGAGTGTGTGCTGGCTAGATCGGA CTGCCCCAGCACCTCGTCCAGGTGCATCAACACTGAAGGTGGCTACGTCTGCAGATGCTCAGAAGGCTAC GAAGGAGACGGGATCTCCTGTTTCGATATTGACGAGTGCCAGCGGGGGGCGCACAACTGCGCTGAGAATG CCGCCTGCACCAACACGGAGGGAGGCTACAACTGCACCTGCGCAGGCCGCCCATCCTCGCCCGGACGGAG TTGCCCTGACTCTACCGCACCCTCTCTCCTTGGGGAAGATGGCCACCATTTGGACCGAAATAGTTATCCA GGATGCCCATCCTCATATGATGGATACTGCCTCAATGGTGGCGTGTGCATGCATATTGAATCACTGGACA GCTACACATGCAACTGTGTTATTGGCTATTCTGGGGATCGATGTCAGACTCGAGACCTACGATGGTGGGA GCTGCGTCATGCTGGCTACGGGCAGAAGCATGACATCATGGTGGTGGCTGTCTGCATGGTGGCACTGGTC CTGCTGCTCCTCTTGGGGATGTGGGGGACTTACTACTACAGGACTCGGAAGCAGCTATCAAACCCCCCAA AGAACCCTTGTGATGAGCCAAGCGGAAGTGTGAGCAGCAGCGGGCCCGACAGCAGCAGCGGGGCAGCTGT GGCTTCTTGTCCCCAACCTTGGTTTGTGGTCCTAGAGAAACACCAAGACCCCAAGAATGGGAGTCTGCCT GCGGATGGTACGAATGGTGCAGTAGTAGATGCTGGCCTGTCTCCCTCCCTGCAGCTCGGGTCAGTGCATC TGACTTCATGGAGACAGAAGCCCCACATAGATGGAATGGGCACAGGGCAAAGCTGCTGGATTCCACCATC AAGTGACAGAGGACCCCAGGAAATAGAGGGAAACTCCCACCTACCCTCCTACAGACCTGTGGGGCCGGAG AAGCTGCATTCTCTCCAGTCAGCTAATGGATCGTGTCACGAAAGGGCTCCAGACCTGCCACGGCAGACAG AGCCAGTTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211815 protein sequence
Red=Cloning site Green=Tags(s) MPWGRRPTWLLLAFLLVFLKISILSVTAWQTGNCQPGPLERSERSGTCAGPAPFLVFSQGKSISRIDPDG TNHQQLVVDAGISADMDIHYKKERLYWVDVERQVLLRVFLNGTGLEKVCNVERKVSGLAIDWIDDEVLWV DQQNGVITVTDMTGKNSRVLLSSLKHPSNIAVDPIERLMFWSSEVTGSLHRAHLKGVDVKTLLETGGISV LTLDVLDKRLFWVQDSGEGSHAYIHSCDYEGGSVRLIRHQARHSLSSMAFFGDRIFYSVLKSKAIWIANK HTGKDTVRINLHPSFVTPGKLMVVHPRAQPRTEDAAKDPDPELLKQRGRPCRFGLCERDPKSHSSACAEG YTLSRDRKYCEDVNECATQNHGCTLGCENTPGSYHCTCPTGFVLLPDGKQCHELVSCPGNVSKCSHGCVL TSDGPRCICPAGSVLGRDGKTCTGCSSPDNGGCSQICLPLRPGSWECDCFPGYDLQSDRKSCAASGPQPL LLFANSQDIRHMHFDGTDYKVLLSRQMGMVFALDYDPVESKIYFAQTALKWIERANMDGSQRERLITEGV DTLEGLALDWIGRRIYWTDSGKSVVGGSDLSGKHHRIIIQERISRPRGIAVHPRARRLFWTDVGMSPRIE SASLQGSDRVLIASSNLLEPSGITIDYLTDTLYWCDTKRSVIEMANLDGSKRRRLIQNDVGHPFSLAVFE DHLWVSDWAIPSVIRVNKRTGQNRVRLQGSMLKPSSLVVVHPLAKPGADPCLYRNGGCEHICQESLGTAR CLCREGFVKAWDGKMCLPQDYPILSGENADLSKEVTSLSNSTQAEVPDDDGTESSTLVAEIMVSGMNYED DCGPGGCGSHARCVSDGETAECQCLKGFARDGNLCSDIDECVLARSDCPSTSSRCINTEGGYVCRCSEGY EGDGISCFDIDECQRGAHNCAENAACTNTEGGYNCTCAGRPSSPGRSCPDSTAPSLLGEDGHHLDRNSYP GCPSSYDGYCLNGGVCMHIESLDSYTCNCVIGYSGDRCQTRDLRWWELRHAGYGQKHDIMVVAVCMVALV LLLLLGMWGTYYYRTRKQLSNPPKNPCDEPSGSVSSSGPDSSSGAAVASCPQPWFVVLEKHQDPKNGSLP ADGTNGAVVDAGLSPSLQLGSVHLTSWRQKPHIDGMGTGQSCWIPPSSDRGPQEIEGNSHLPSYRPVGPE KLHSLQSANGSCHERAPDLPRQTEPVQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010113 |
| ORF Size | 3654 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010113.1, NM_010113.2, NM_010113.3, NM_010113.4, NP_034243.1 |
| RefSeq Size | 4757 bp |
| RefSeq ORF | 3654 bp |
| Locus ID | 13645 |
| UniProt ID | P01132 |
| MW | 133.1 kDa |
| Gene Summary | This gene encodes epidermal growth factor (EGF), the founding member of the EGF family of growth factors that are implicated in cell proliferation and differentiation. The encoded protein can localize to the membrane and function in juxtacrine signaling or undergo proteolytic processing to generate a soluble form of the hormone. Mice lacking the encoded protein do not exhibit an abnormal phenotype but transgenic mice overexpressing the encoded protein exhibit hypospermatogenesis. [provided by RefSeq, Jul 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203560 | Egf (untagged) - Mouse epidermal growth factor (Egf), (10ug) |
CNY 12,720.00 |
|
| MG211815 | Egf (tGFP-tagged) - Mouse epidermal growth factor (Egf) |
CNY 13,400.00 |
|
| MR211815L3 | Lenti ORF clone of Egf (Myc-DDK-tagged) - Mouse epidermal growth factor (Egf) |
CNY 14,060.00 |
|
| MR211815L4 | Lenti ORF clone of Egf (mGFP-tagged) - Mouse epidermal growth factor (Egf) |
CNY 14,060.00 |
