Col5a1 (NM_015734) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI413331
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR221861 representing NM_015734 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGTACACACCCGCTGGAAAGCTGCGCGCCCAGGCGCCCTGCTGCTGTCTTCGCCGCTACTCCTGT TCCTGCTGCTGCTGTGGGCGCCGCCTTCGAGCCGCGCAGCTCAGCCAGCAGATCTTCTGGAGATGCTAGA TTTTCACAATTTGCCCTCAGGGGTAACGAAAACCACAGGTTTCTGTGCTACTCGAAGATCTTCCAGCGAG CCGGATGTTGCCTACCGAGTCTCTAAAGATGCACAGCTCAGCATGCCCACCAAGCAGCTGTACCCTGAGT CTGGTTTTCCCGAGGACTTCTCCATCCTGACAACCGTGAAAGCCAAGAAAGGCAGCCAGGCCTTCCTAGT CTCCATTTACAATGAGCAGGGCATCCAGCAGTTGGGGCTGGAGCTGGGCCGCTCCCCTGTCTTCCTCTAT GAGGACCACACAGGGAAGCCCGGGCCTGAAGAGTATCCGCTTTTCCCTGGCATCAACTTGTCCGATGGCA AGTGGCACCGAATTGCTCTCAGTGTCTACAAGAAAAATGTCACCTTGATCCTCGACTGTAAGAAGAAGAT TACGAAGTTCCTCAGCCGCAGTGACCACCCCATAATAGACACCAATGGGATTGTCATGTTTGGCTCCCGG ATTCTGGATGATGAAATATTTGAGGGTGACATCCAACAGTTGCTTTTCGTCTCTGACAACCGAGCTGCCT ATGACTACTGTGAGCACTACAGCCCCGACTGTGACACTGCGGTCCCTGACACACCTCAGTCACAGGACCC TAACCCGGATGAATATTACCCAGAAGGAGAGGGTGAGACCTATTACTATGAGTATCCATATTATGAAGAC CCTGAAGACCCGGGAAAGGAGCCTGCCCCTACTCAGAAGCCAGTGGAAGCTGCCAGAGAAACCACAGAGG TTCCTGAGGAGCAGACCCAGCCCCTACCCGAAGCCCCTACAGTGCCTGAGACCAGTGACACGGCTGACAA GGAGGACAGTCTAGGGATCGGGGACTATGACTACGTGCCCCCAGATGACTATTACACTCCACCCCCATAT GAAGACTTTGGATATGGCGAGGGTGTGGAGAACCCTGACCAGCCCACCAACCCCGACTCAGGGGCTGAGG TCCCCACCAGCACCACTGTTACCTCCAACACCTCCAATCCAGCTCCAGGAGAAGGGAAGGATGACCTGGG CGGCGAATTCACCGAGGAAACCATCAAGAATCTAGAGGAAAACTACTATGACCCGTACTTTGACCCCGAC TCCGACTCCAGTGTCTCTCCATCAGAGATAGGGCCAGGCATGCCCGCTAACCAGGACACCATCTTTGAGG GGATTGGAGGACCCCGAGGTGAGAAAGGGCAAAAGGGAGAACCAGCCATCATTGAGCCGGGGATGCTGAT CGAGGGGCCCCCTGGCCCTGAAGGCCCTGCTGGTCTTCCAGGACCTCCAGGAACTACAGGTCCTACTGGC CAAATGGGTGACCCTGGAGAAAGGGGTCCCCCTGGGCGCCCAGGTCTTCCTGGAGCTGATGGCTTGCCTG GCCCCCCAGGTACCATGCTCATGCTGCCGTTCCGGTTTGGAGGCGGTGGCGATGCCGGTTCTAAGGGCCC CATGGTCTCTGCGCAGGAGTCCCAGGCCCAGGCTATCCTCCAGCAAGCCAGGTTGGCACTGAGGGGACCA GCTGGCCCAATGGGTCTCACCGGGAGACCTGGCCCCATGGGTCCTCCTGGGAGTGGCGGTTTGAAAGGTG AGCCAGGAGACATGGGACCTCAGGGTCCTCGAGGTGTGCAAGGCCCACCTGGCCCAACAGGGAAGCCTGG AAGACGGGGCCGTGCTGGAAGTGATGGAGCCAGAGGCATGCCTGGACAAACAGGCCCCAAGGGTGACCGT GGCTTTGATGGTCTGGCTGGGTTGCCGGGAGAGAAAGGCCATAGAGGTGACCCTGGTCCTTCTGGCCCGC CCGGAATCCCAGGAGATGATGGAGAAAGGGGTGACGATGGAGAAGTTGGGCCCAGGGGACTGCCCGGGGA GCCTGGACCACGTGGTCTGCTTGGGCCAAAAGGCCCCCCAGGGCCTCCTGGACCTCCTGGTGTAACGGGT ATGGATGGCCAGCCTGGCCCAAAAGGAAATGTGGGTCCCCAGGGAGAGCCTGGGCCGCCAGGACAGCAGG GTAATCCTGGTGCCCAGGGTCTTCCAGGTCCCCAGGGTGCCATTGGTCCTCCAGGAGAAAAGGGTCCTTT GGGGAAACCAGGTCTCCCAGGAATGCCAGGCGCTGATGGACCCCCGGGGCACCCTGGAAAAGAAGGTCCT CCAGGAGAGAAAGGAGGCCAGGGTCCTCCTGGCCCCCAGGGTCCCATTGGCTACCCCGGTCCACGAGGAG TCAAGGGGGCAGATGGCATCCGAGGTCTGAAGGGCACCAAGGGGGAGAAGGGTGAAGACGGCTTCCCTGG GTTTAAAGGCGACATGGGAATAAAGGGTGACCGGGGGGAAATCGGCCCACCTGGTCCCCGAGGAGAAGAT GGTCCTGAAGGTCCAAAGGGTCGAGGTGGTCCCAATGGTGATCCTGGTCCCCTGGGGCCCACTGGGGAAA AGGGAAAGCTTGGCGTGCCCGGATTACCGGGGTACCCAGGAAGACAAGGGCCAAAGGGTTCCATTGGATT CCCTGGCTTCCCGGGCGCCAACGGAGAGAAGGGTGGCAGGGGGACACCTGGAAAGCCAGGACCACGGGGA CAGAGAGGCCCAACGGGCCCGCGGGGTGAACGAGGCCCACGAGGCATCACGGGGAAGCCTGGCCCTAAGG GCAACTCCGGAGGTGATGGCCCAGCTGGCCCTCCTGGTGAACGGGGACCCAACGGACCCCAAGGTCCCAC CGGCTTTCCTGGACCCAAGGGTCCCCCGGGCCCACCAGGCAAGGACGGACTCCCTGGACACCCTGGGCAG AGAGGGGAGACCGGTTTCCAAGGCAAGACTGGCCCTCCAGGGCCCCCAGGAGTGGTTGGCCCTCAGGGTC CCACAGGAGAGACGGGCCCCATGGGTGAGCGTGGCCATCCTGGTCCTCCAGGCCCTCCTGGTGAACAGGG CCTCCCAGGTGCTGCTGGGAAAGAAGGAACGAAGGGTGACCCAGGTCCTGCTGGCCTCCCTGGGAAGGAT GGCCCTCCAGGATTGCGTGGATTCCCTGGGGACCGAGGGCTACCTGGCCCCGTGGGAGCCCTTGGACTCA AAGGCAGTGAAGGCCCCCCTGGCCCACCAGGTCCTGCGGGTTCTCCAGGGGAGAGAGGACCAGCTGGTGC CGCTGGGCCCATCGGAATTCCAGGGAGACCTGGGCCTCAGGGACCTCCGGGGCCTGCTGGAGAGAAAGGA CTTCCTGGCGAGAAAGGTCCACAAGGCCCAGCTGGCCGAGATGGCCTCCAAGGTCCCGTGGGGCTCCCTG GACCAGCCGGCCCAGTGGGTCCTCCTGGAGAAGATGGAGATAAGGGAGAGATCGGAGAGCCAGGGCAGAA GGGAAGCAAGGGCGACAAAGGCGAGCAGGGTCCTCCTGGGCCTACCGGTCCTCAAGGCCCGATTGGACAG CCAGGCCCTTCGGGAGCAGATGGTGAACCTGGCCCTCGTGGACAGCAGGGCCTGTTTGGGCAGAAAGGAG ATGAAGGTTCAAGAGGTTTCCCAGGACCCCCCGGGCCAGTGGGATTGCAGGGTTTGCCAGGACCTCCAGG AGAAAAGGGCGAGACAGGAGACGTGGGCCAGATGGGCCCTCCTGGTCCACCAGGCCCCCGAGGACCCTCT GGAGCTCCAGGTGCCGATGGACCACAGGGTCCTCCTGGAGGGATTGGCAACCCTGGTGCAGTCGGAGAAA AGGGAGAACCTGGTGAAGCTGGAGATCCTGGCCTTCCAGGAGAAGGAGGTCCCCTGGGACCTAAAGGAGA AAGAGGGGAGAAGGGAGAGGCTGGCCCCTCTGGTGCTGCTGGACCCCCTGGACCCAAAGGCCCTCCTGGA GATGATGGCCCCAAAGGCAGCCCTGGCCCTGTGGGCTTTCCTGGAGATCCTGGTCCCCCTGGAGAGCCAG GCCCCGCAGGTCAAGACGGCCCACCTGGTGACAAAGGGGACGATGGTGAACCTGGGCAGACGGGGTCCCC GGGCCCTACTGGTGAACCTGGTCCATCTGGGCCTCCAGGAAAGAGGGGTCCCCCAGGCCCTGCAGGCCCT GAAGGCAGGCAGGGGGAGAAAGGAGCCAAGGGAGAAGCTGGCTTAGAAGGCCCTCCTGGGAAGACTGGCC CCATTGGCCCCCAAGGGGCCCCTGGGAAGCCTGGCCCCGATGGTCTCCGTGGAATCCCTGGTCCTGTGGG TGAGCAAGGCCTCCCAGGATCCCCAGGCCCCGATGGTCCACCCGGCCCCATGGGTCCTCCAGGACTCCCT GGCCTCAAAGGAGACTCCGGTCCCAAAGGTGAAAAGGGCCATCCAGGCCTGATTGGACTCATCGGCCCTC CGGGAGAGCAAGGTGAAAAGGGTGACCGTGGACTCCCAGGCCCCCAGGGTTCATCTGGTCCTAAAGGAGA TCAGGGCATCACAGGTCCTTCTGGCCCACTTGGGCCTCCTGGTCCTCCTGGCTTGCCGGGCCCTCCAGGT CCCAAAGGTGCTAAGGGCTCTTCGGGTCCCACCGGCCCGAAGGGTGAGGCAGGCCACCCAGGACTCCCCG GCCCACCTGGCCCTCCGGGTGAGGTCATCCAGCCCCTGCCAATCCAGGCCTCCAGGACTCGGCGGAACAT TGATGCCAGCCAGCTCCTGGACGATGGGGCTGGGGAGAGCTACGTGGATTATGCAGATGGCATGGAAGAG ATCTTTGGTTCCCTCAACTCCCTGAAGCTGGAGATTGAACAGATGAAGCGACCACTGGGCACCCAGCAGA ACCCAGCCCGTACCTGCAAGGATCTACAGCTCTGTCATCCTGACTTCCCAGATGGCGAATACTGGGTCGA TCCCAACCAAGGGTGCTCCAGGGACTCCTTCAAAGTCTACTGCAATTTCACAGCTGGAGGGTCCACGTGC GTCTTCCCTGACAAGAAGTCTGAGGGAGCCAGAATCACTTCTTGGCCCAAAGAAAACCCAGGTTCCTGGT TCAGTGAATTCAAGCGTGGGAAACTGCTCTCCTATGTGGATGCTGAAGGCAACCCCGTGGGCGTGGTACA AATGACCTTCCTGCGGCTGCTGAGCGCCTCTGCCCACCAGAACGTCACCTACAACTGCTACCAGTCCGTG GCCTGGCAGGATGCCGCCACAGGCAGCTATGATAAGGCTATCCGCTTCTTGGGCTCCAACGATGAGGAAA TGTCTTATGATAACAACCCCTACATCCGTGCCCTGGTGGATGGCTGTGCTACCAAGAAAGGCTACCAGAA GACGGTGCTGGAGATCGACACGCCCAAAGTAGAGCAAGTCCCCATTGTGGACATCATGTTCAACGACTTT GGCGAAGCCTCACAGAAATTTGGATTTGAAGTGGGGCCAGCTTGCTTCCTAGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR221861 representing NM_015734 Red=Cloning site Green=Tags(s) MDVHTRWKAARPGALLLSSPLLLFLLLLWAPPSSRAAQPADLLEMLDFHNLPSGVTKTTGFCATRRSSSE PDVAYRVSKDAQLSMPTKQLYPESGFPEDFSILTTVKAKKGSQAFLVSIYNEQGIQQLGLELGRSPVFLY EDHTGKPGPEEYPLFPGINLSDGKWHRIALSVYKKNVTLILDCKKKITKFLSRSDHPIIDTNGIVMFGSR ILDDEIFEGDIQQLLFVSDNRAAYDYCEHYSPDCDTAVPDTPQSQDPNPDEYYPEGEGETYYYEYPYYED PEDPGKEPAPTQKPVEAARETTEVPEEQTQPLPEAPTVPETSDTADKEDSLGIGDYDYVPPDDYYTPPPY EDFGYGEGVENPDQPTNPDSGAEVPTSTTVTSNTSNPAPGEGKDDLGGEFTEETIKNLEENYYDPYFDPD SDSSVSPSEIGPGMPANQDTIFEGIGGPRGEKGQKGEPAIIEPGMLIEGPPGPEGPAGLPGPPGTTGPTG QMGDPGERGPPGRPGLPGADGLPGPPGTMLMLPFRFGGGGDAGSKGPMVSAQESQAQAILQQARLALRGP AGPMGLTGRPGPMGPPGSGGLKGEPGDMGPQGPRGVQGPPGPTGKPGRRGRAGSDGARGMPGQTGPKGDR GFDGLAGLPGEKGHRGDPGPSGPPGIPGDDGERGDDGEVGPRGLPGEPGPRGLLGPKGPPGPPGPPGVTG MDGQPGPKGNVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKPGLPGMPGADGPPGHPGKEGP PGEKGGQGPPGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGIKGDRGEIGPPGPRGED GPEGPKGRGGPNGDPGPLGPTGEKGKLGVPGLPGYPGRQGPKGSIGFPGFPGANGEKGGRGTPGKPGPRG QRGPTGPRGERGPRGITGKPGPKGNSGGDGPAGPPGERGPNGPQGPTGFPGPKGPPGPPGKDGLPGHPGQ RGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGAAGKEGTKGDPGPAGLPGKD GPPGLRGFPGDRGLPGPVGALGLKGSEGPPGPPGPAGSPGERGPAGAAGPIGIPGRPGPQGPPGPAGEKG LPGEKGPQGPAGRDGLQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGSKGDKGEQGPPGPTGPQGPIGQ PGPSGADGEPGPRGQQGLFGQKGDEGSRGFPGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPPGPRGPS GAPGADGPQGPPGGIGNPGAVGEKGEPGEAGDPGLPGEGGPLGPKGERGEKGEAGPSGAAGPPGPKGPPG DDGPKGSPGPVGFPGDPGPPGEPGPAGQDGPPGDKGDDGEPGQTGSPGPTGEPGPSGPPGKRGPPGPAGP EGRQGEKGAKGEAGLEGPPGKTGPIGPQGAPGKPGPDGLRGIPGPVGEQGLPGSPGPDGPPGPMGPPGLP GLKGDSGPKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGSSGPKGDQGITGPSGPLGPPGPPGLPGPPG PKGAKGSSGPTGPKGEAGHPGLPGPPGPPGEVIQPLPIQASRTRRNIDASQLLDDGAGESYVDYADGMEE IFGSLNSLKLEIEQMKRPLGTQQNPARTCKDLQLCHPDFPDGEYWVDPNQGCSRDSFKVYCNFTAGGSTC VFPDKKSEGARITSWPKENPGSWFSEFKRGKLLSYVDAEGNPVGVVQMTFLRLLSASAHQNVTYNCYQSV AWQDAATGSYDKAIRFLGSNDEEMSYDNNPYIRALVDGCATKKGYQKTVLEIDTPKVEQVPIVDIMFNDF GEASQKFGFEVGPACFLG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_015734
ORF Size	5514 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_015734.2, NP_056549.2
RefSeq Size	8420 bp
RefSeq ORF	5517 bp
Locus ID	12831
UniProt ID	O88207
MW	183.7 kDa
Gene Summary	Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin (By similarity). Transcriptionally activated by CEBPZ, which recognizes a CCAAT-like motif, CAAAT in the COL5A1 promoter.[UniProtKB/Swiss-Prot Function]

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