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Kat2a (NM_020004) Mouse Tagged ORF Clone
CAT＃: MR222236
- TrueORF®
Kat2a (Myc-DDK-tagged) - Mouse K(lysine) acetyltransferase 2A (Kat2a), transcript variant 1
ORF Plasmid: tGFP
"NM_020004" in other vectors (4)
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CNY 8,270.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110051E14Rik; AW212720; Gcn5; Gcn5l2; mmGCN5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222236 representing NM_020004
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAACCTTCCCAGGCCCCAAACCCGGTCCCGGCAGCGCAGCCCCGGCCCCTTCACTCCCCAGCCC CTGCCCCAACTTCGACTCCTGCGCCCAGTCCGGCTTCAGCCTCCACTCCGGCTCCGACTCCGGCACCAGC CCCTGCCCCAGCTGCAGCCCCAGCTGGGAGCACAGGGAGTGGGGGCGCCGGGGTAGGAAGTGGGGGGGAT CCGGCTCGACCTGGCCTGAGCCAGCAGCAGCGCGCTAGCCAGAGGAAGGCGCAAGTCCGGGGTCTGCCGC GTGCCAAGAAGCTTGAGAAACTAGGGGTCTTCTCAGCTTGCAAGGCCAATGAAACCTGCAAGTGTAATGG CTGGAAAAACCCCAAGCCCCCCACCGCACCCCGCATGGACCTCCAGCAGCCAGCTGCCAACCTGAGCGAG TTGTGCCGTAGCTGTGAGCACCCTTTGGCGGACCATGTGTCCCACCTGGAGAATGTGTCAGAGGACGAGA TTAACCGGCTCTTGGGAATGGTAGTAGATGTAGAAAATCTGTTCATGTCTGTTCACAAGGAAGAGGACAC AGACACCAAACAAGTCTATTTCTACCTCTTCAAGCTCTTGCGGAAGTGCATTCTGCAGATGACACGGCCA GTGGTGGAGGGGTCTCTAGGCAGCCCCCCATTTGAGAAGCCTAACATTGAACAGGGTGTACTGAACTTTG TGCAGTACAAATTTAGCCACCTGGCCCCCCGAGAGCGCCAGACAATGTTTGAGCTCTCAAAGATGTTCCT GCTCTGCCTTAACTACTGGAAGCTGGAGACCCCTGCTCAATTCCGGCAGCGGTCCCAGTCTGAGGATGTC GCTACCTACAAAGTCAATTATACCAGATGGCTCTGTTACTGCCACGTGCCCCAGAGCTGCGACAGCCTCC CCCGCTACGAGACCACCCACGTGTTTGGCCGAAGCCTTCTACGGTCCATTTTCACTGTCACCCGCCGGCA GCTACTGGAAAAATTTCGGGTGGAAAAGGACAAACTGGTGCCTGAGAAGAGGACCCTCATCCTCACTCAC TTCCCCAAATTCCTGTCCATGCTTGAGGAAGAGATCTACGGGGCAAATTCTCCCATCTGGGAGTCAGGCT TCACCATGCCACCTTCGGAGGGGACACAGCTGGTGCCCCGGCCAGCCACGGTCAGTGCAACAGTTGTACC CAGCTTCAGTCCTTCCATGGGAGGAGGCAGCAACAGCTCCCTGAGCCTAGATTCCGCAGGGACTGAGCCC ATGCCAGGGGAGAAGAGGAAGCTTCCTGAGAACCTGACCCTGGAGGATGCCAAGCGGCTCCGCGTGATGG GGGACATCCCCATGGAGCTGGTCAATGAGGTCATGCTGACGATCACTGACCCCGCTGCCATGCTGGGGCC TGAGACAAGCCTGCTTTCGGCCAACGCAGCTCGAGATGAGACGGCTCGCCTGGAAGAACGGCGAGGAATC ATCGAGTTCCACGTCATTGGCAACTCCCTGACGCCCAAGGCCAACCGGCGCGTGCTGCTCTGGCTCGTGG GGCTGCAGAATGTCTTTTCCCACCAGCTGCCAAGAATGCCCAAGGAATATATCGCCCGCCTCGTCTTTGA CCCGAAGCACAAGACCCTGGCCCTGATCAAGGATGGGCGGGTCATCGGTGGCATTTGCTTCCGCATGTTT CCCACTCAGGGCTTCACGGAAATCGTCTTCTGTGCCGTCACCTCAAATGAGCAGGTCAAGGGCTATGGCA CCCACCTGATGAACCACCTAAAGGAGTACCACATCAAACACAGCATCCTTTACTTTCTCACCTATGCTGA CGAGTACGCCATTGGCTACTTCAAAAAGCAGGGCTTCTCCAAAGACATCAAGGTGCCCAAGAGCCGCTAT CTGGGCTACATCAAGGACTATGAAGGAGCGACGCTGATGGAGTGTGAGCTGAATCCCCGAATCCCCTACA CAGAGCTGTCCCACATCATCAAGAAACAGAAAGAGATCATCAAGAAGTTGATTGAGCGCAAACAGGCACA GATCCGCAAGGTCTACCCTGGACTCAGCTGCTTCAAAGAGGGAGTGAGGCAGATCCCGGTGGAGAGCGTC CCTGGCATTCGAGAGACAGGCTGGAAGCCACTGGGAAAGGAGAAGGGCAAGGAGCTTAAGGATCCTGATC AGCTATATACGACCCTCAAAAACCTGCTGGCTCAGATCAAGTCACACCCCAGTGCCTGGCCCTTCATGGA ACCTGTGAAGAAGTCAGAGGCTCCTGACTACTACGAGGTTATCCGTTTCCCCATTGACCTGAAGACCATG ACAGAACGGTTGCGCAGTCGCTACTATGTGACCCGGAAGCTCTTCGTAGCTGACCTGCAGCGGGTCATCG CCAACTGCCGGGAGTACAATCCCCCAGACAGCGAATACTGTCGCTGCGCCAGTGCCCTGGAGAAGTTCTT CTACTTCAAGCTCAAAGAGGGAGGGCTCATCGACAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222236 representing NM_020004
Red=Cloning site Green=Tags(s) MAEPSQAPNPVPAAQPRPLHSPAPAPTSTPAPSPASASTPAPTPAPAPAPAAAPAGSTGSGGAGVGSGGD PARPGLSQQQRASQRKAQVRGLPRAKKLEKLGVFSACKANETCKCNGWKNPKPPTAPRMDLQQPAANLSE LCRSCEHPLADHVSHLENVSEDEINRLLGMVVDVENLFMSVHKEEDTDTKQVYFYLFKLLRKCILQMTRP VVEGSLGSPPFEKPNIEQGVLNFVQYKFSHLAPRERQTMFELSKMFLLCLNYWKLETPAQFRQRSQSEDV ATYKVNYTRWLCYCHVPQSCDSLPRYETTHVFGRSLLRSIFTVTRRQLLEKFRVEKDKLVPEKRTLILTH FPKFLSMLEEEIYGANSPIWESGFTMPPSEGTQLVPRPATVSATVVPSFSPSMGGGSNSSLSLDSAGTEP MPGEKRKLPENLTLEDAKRLRVMGDIPMELVNEVMLTITDPAAMLGPETSLLSANAARDETARLEERRGI IEFHVIGNSLTPKANRRVLLWLVGLQNVFSHQLPRMPKEYIARLVFDPKHKTLALIKDGRVIGGICFRMF PTQGFTEIVFCAVTSNEQVKGYGTHLMNHLKEYHIKHSILYFLTYADEYAIGYFKKQGFSKDIKVPKSRY LGYIKDYEGATLMECELNPRIPYTELSHIIKKQKEIIKKLIERKQAQIRKVYPGLSCFKEGVRQIPVESV PGIRETGWKPLGKEKGKELKDPDQLYTTLKNLLAQIKSHPSAWPFMEPVKKSEAPDYYEVIRFPIDLKTM TERLRSRYYVTRKLFVADLQRVIANCREYNPPDSEYCRCASALEKFFYFKLKEGGLIDK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020004 |
| ORF Size | 2487 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020004.1, NM_020004.2, NM_020004.3, NM_020004.4, NM_020004.5, NP_064388.2 |
| RefSeq Size | 3048 bp |
| RefSeq ORF | 2493 bp |
| Locus ID | 14534 |
| UniProt ID | Q9JHD2 |
| MW | 93.8 kDa |
| Gene Summary | Protein lysine acyltransferase that can act both as a acetyltransferase and succinyltransferase, depending on the context (PubMed:28424240). Acts as a histone lysine succinyltransferase: catalyzes succinylation of histone H3 on 'Lys-79' (H3K79succ), with a maximum frequency around the transcription start sites of genes (By similarity). Succinylation of histones gives a specific tag for epigenetic transcription activation (By similarity). Association with the 2-oxoglutarate dehydrogenase complex, which provides succinyl-CoA, is required for histone succinylation (By similarity). In different complexes, functions either as an acetyltransferase (HAT) or as a succinyltransferase: in the SAGA and ATAC complexes, acts as a histone acetyltransferase (By similarity). Has significant histone acetyltransferase activity with core histones, but not with nucleosome core particles (By similarity). Acetylation of histones gives a specific tag for epigenetic transcription activation (PubMed:28424240). Involved in long-term memory consolidation and synaptic plasticity: acts by promoting expression of a hippocampal gene expression network linked to neuroactive receptor signaling (PubMed:25024434). Acts as a positive regulator of T-cell activation: upon TCR stimulation, recruited to the IL2 promoter following interaction with NFATC2 and catalyzes acetylation of histone H3 at Lys-9 (H3K9ac), leading to promote IL2 expression (PubMed:28424240). Also acetylates non-histone proteins, such as CEBPB, PLK4 and TBX5 (PubMed:17301242). Involved in heart and limb development by mediating acetylation of TBX5, acetylation regulating nucleocytoplasmic shuttling of TBX5 (By similarity). Acts as a negative regulator of centrosome amplification by mediating acetylation of PLK4 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204844 | Kat2a (untagged) - Mouse K(lysine) acetyltransferase 2A (Kat2a), transcript variant 1, (10ug) |
CNY 2,000.00 |
|
| MG222236 | Kat2a (tGFP-tagged) - Mouse K(lysine) acetyltransferase 2A (Kat2a) transcript variant 1, (10ug) |
CNY 9,120.00 |
|
| MR222236L3 | Lenti ORF clone of Kat2a (Myc-DDK-tagged) - Mouse K(lysine) acetyltransferase 2A (Kat2a), transcript variant 1 |
CNY 10,170.00 |
|
| MR222236L4 | Lenti ORF clone of Kat2a (mGFP-tagged) - Mouse K(lysine) acetyltransferase 2A (Kat2a), transcript variant 1 |
CNY 10,170.00 |
