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Aga (NM_001005847) Mouse Tagged ORF Clone
CAT＃: MR224201
- TrueORF®
Aga (Myc-DDK-tagged) - Mouse aspartylglucosaminidase (Aga), transcript variant 1
ORF Plasmid: tGFP
"NM_001005847" in other vectors (4)
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CNY 2,850.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW060726 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224201 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGGAAGTCGAATCTGTCTCTGCTTCTCCTACTGCTGGTCCTGGGCATGCCCCTGGTGCGGGGCT CCAGCCCTCTGCCCCTGGTCGTCAACACTTGGCCTTTTAAGAATGCCACTGAAGCAGCGTGGTGGACATT GCTATCTGGAGGTTCTGCCCTGGATGCAGTGGAGAACGGCTGTGCTGTGTGTGAGAAGGAGCAGTGTGAT GGGACTGTAGGCTTTGGAGGAAGTCCTGATGAAGGTGGCGAAACCACCCTGGATGCCATGATAATGGATG GCACTGCCATGGATGTGGGAGCAGTGGGAGGCCTTAGAAGAATTAAAAACGCGATTGGCGTGGCGCGGAG AGTCCTGGAGCATACCACACACACGCTTTTAGTGGGGGACTCAGCCACCAAGTTTGCTGAAAGTATGGGG TTTACTAATGAGGACTTGTCTACCAAAACCTCAAGAGATCTTCATTCAGATTGGCTTTCTCGAAATTGCC AGCCAAATTATTGGAGAAATGTTATTCCAGATCCCTCAAAATACTGTGGACCCTACAAACCATCTGGTTT CTTAAAGCAGAGTATTTCTCCCCACAAAGAAGAAGTGGATATCCACAGCCATGATACTATTGGCATGGTT GTAATCCATAAGACGGGACATACTGCTGCTGGCACATCCACAAATGGTATAAAATTCAAAATACCTGGTC GTGTAGGGGATTCACCAATCCCTGGAGCCGGAGCCTATGCTGATGACACGGCTGGAGCAGCTGCAGCCAC TGGCGATGGTGACACACTCCTGCGCTTTCTGCCGAGCTACCAAGCTGTAGAATATATGAGAGGAGGAGAT GACCCAGCCATAGCTTGCCAAAAAGTGATTTTAAGAATTCAGAAATACTATCCAAACTTCTTTGGAGCGG TCATATGTGCCAGTGTGAACGGAAGTTATGGTGCTGCTTGCAACAAACTTCCAACATTTACACAATTTAG TTTCATGGTTTCTAATTCTTTACACAATGAGCCAACCGAAAAAAAAGTAGACTGCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224201 protein sequence
Red=Cloning site Green=Tags(s) MERKSNLSLLLLLLVLGMPLVRGSSPLPLVVNTWPFKNATEAAWWTLLSGGSALDAVENGCAVCEKEQCD GTVGFGGSPDEGGETTLDAMIMDGTAMDVGAVGGLRRIKNAIGVARRVLEHTTHTLLVGDSATKFAESMG FTNEDLSTKTSRDLHSDWLSRNCQPNYWRNVIPDPSKYCGPYKPSGFLKQSISPHKEEVDIHSHDTIGMV VIHKTGHTAAGTSTNGIKFKIPGRVGDSPIPGAGAYADDTAGAAAATGDGDTLLRFLPSYQAVEYMRGGD DPAIACQKVILRIQKYYPNFFGAVICASVNGSYGAACNKLPTFTQFSFMVSNSLHNEPTEKKVDCI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001005847 |
| ORF Size | 1041 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001005847.2, NP_001005847.1 |
| RefSeq Size | 1307 bp |
| RefSeq ORF | 1041 bp |
| Locus ID | 11593 |
| UniProt ID | Q64191 |
| MW | 37 kDa |
| Gene Summary | This gene encodes an amidase enzyme that participates in the breakdown of glycoproteins in the cell. The encoded protein undergoes proteolytic processing to generate a mature enzyme. Mice lacking the encoded protein exhibit accumulation of aspartylglucosamine along with lysosomal vacuolization, axonal swelling in the gracile nucleus and impaired neuromotor coordination. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Oct 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208116 | Aga (untagged) - Mouse aspartylglucosaminidase (Aga), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
| MG224201 | Aga (tGFP-tagged) - Mouse aspartylglucosaminidase (Aga), (10ug) |
CNY 3,140.00 |
|
| MR224201L3 | Lenti ORF clone of Aga (Myc-DDK-tagged) - Mouse aspartylglucosaminidase (Aga), transcript variant 1 |
CNY 4,750.00 |
|
| MR224201L4 | Lenti ORF clone of Aga (mGFP-tagged) - Mouse aspartylglucosaminidase (Aga), transcript variant 1 |
CNY 4,750.00 |
