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Il1rap (NM_001159318) Mouse Tagged ORF Clone
CAT＃: MR224562
- TrueORF®
Il1rap (Myc-DDK-tagged) - Mouse interleukin 1 receptor accessory protein (Il1rap), transcript variant 4
ORF Plasmid: tGFP
"NM_001159318" in other vectors (3)
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CNY 7,528.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 6430709H04Rik; AI255955; AV239853; IL-1RAcP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224562 representing NM_001159318
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACTTCTGTGGTATTTGATGAGTCTGTCCTTCTATGGGATCCTGCAGAGTCATGCTTCGGAGCGCT GTGATGACTGGGGACTAGATACCATGCGACAAATCCAAGTGTTTGAAGATGAGCCGGCTCGAATCAAGTG CCCCCTCTTTGAACACTTCCTGAAGTACAACTACAGCACTGCCCATTCCTCTGGCCTTACCCTGATCTGG TACTGGACCAGGCAAGACCGGGACCTGGAGGAGCCCATTAACTTCCGCCTCCCAGAGAATCGCATCAGTA AGGAGAAAGATGTGCTCTGGTTCCGGCCCACCCTCCTCAATGACACGGGCAATTACACCTGCATGTTGAG GAACACAACTTACTGCAGCAAAGTTGCATTTCCCCTGGAAGTTGTTCAGAAGGACAGCTGTTTCAATTCT GCCATGAGATTCCCAGTGCACAAGATGTATATTGAACATGGCATTCATAAGATCACATGTCCAAATGTAG ACGGATACTTTCCTTCCAGTGTCAAACCATCGGTCACTTGGTATAAGGGTTGTACTGAAATAGTGGACTT TCATAATGTACTACCCGAGGGCATGAACTTGAGCTTTTTCATCCCCTTGGTTTCAAATAACGGAAATTAC ACATGTGTGGTTACATATCCTGAAAACGGACGTCTCTTTCACCTCACCAGGACTGTGACTGTAAAGGTGG TGGGCTCACCAAAGGATGCATTGCCACCCCAGATCTATTCTCCAAATGACCGTGTTGTCTATGAGAAAGA ACCAGGAGAGGAACTGGTTATTCCCTGCAAAGTCTATTTCAGTTTCATTATGGACTCCCACAATGAGGTC TGGTGGACCATTGATGGAAAGAAGCCTGATGACGTCACAGTCGACATCACTATTAATGAAAGTGTAAGTT ATTCTTCAACGGAAGATGAAACAAGGACTCAGATTTTGAGCATCAAGAAAGTCACCCCGGAGGATCTCAG GCGCAACTATGTCTGTCATGCTCGAAATACCAAAGGGGAAGCTGAGCAGGCTGCCAAGGTGAAACAGAAA GTCATACCACCAAGGTACACAGTAGAACTCGCCTGTGGTTTTGGAGCCACGGTCTTTCTGGTAGTGGTTC TCATTGTGGTTTACCATGTTTACTGGCTGGAGATGGTCCTCTTTTACCGAGCTCACTTTGGAACAGATGA AACAATTCTTGATGGAAAGGAGTATGATATTTATGTTTCCTATGCAAGAAATGTGGAAGAAGAGGAATTT GTGCTGCTGACGCTGCGTGGAGTTTTGGAGAATGAGTTTGGATACAAGCTGTGCATCTTCGACAGAGACA GCCTGCCTGGGGGAAATACCGTGGAAGCAGTTTTTGATTTCATTCAGAGGAGCCGAAGGATGATTGTTGT CCTGAGCCCTGACTATGTGACAGAAAAGAGCATCAGCATGCTGGAGTTTAAGCTGGGTGTCATGTGCCAG AACTCCATTGCCACTAAGCTCATTGTGGTGGAGTACCGTCCGCTTGAGCAACCCCATCCAGGCATCATGC AGCTGAAGGAGTCTGTGTCTTTTGTAAGCTGGAAGGGAGAAAAGTCCAAACATTCTGGCTCCAAGTTCTG GAAGGCCTTGCGTTTGGCTCTTCCCCTGAGAAGTCTGAGCGCCAGCTCCGGCTGGAATGAGAGCTGTTCT TCTCAGTCTGACATCAGTCTGGATCATGTTCAGAGGAGAAGTCGTTTGAAAGAGCCCCCAGAACTCCGAA GCTCAGAGAGGGTGTCTGGAGCAGAGCCAGCCCCGGGCACGATGTCCAAGCACCGAGGGAAACCCTCAGC AGCCTGTCGCTGCTGTGTCACCTACTGTGAAGGAGAAAGTCACCTCAGGAGCAAGAGCCGGGCAGAGATG CACACGCATCCCCAGTGGGAAACACACCTCTGTAAGCCTCCTCTCCAAGAGTCTGAAAGTCAGTGGATAC AAAATGGCACCCGACCCGAACCCGCTCCCCAGATCTCAGCTCTTGCACTCCGCCACTTTACAGATTTATC CAATAACAATGACTTTTATATCCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224562 representing NM_001159318
Red=Cloning site Green=Tags(s) MGLLWYLMSLSFYGILQSHASERCDDWGLDTMRQIQVFEDEPARIKCPLFEHFLKYNYSTAHSSGLTLIW YWTRQDRDLEEPINFRLPENRISKEKDVLWFRPTLLNDTGNYTCMLRNTTYCSKVAFPLEVVQKDSCFNS AMRFPVHKMYIEHGIHKITCPNVDGYFPSSVKPSVTWYKGCTEIVDFHNVLPEGMNLSFFIPLVSNNGNY TCVVTYPENGRLFHLTRTVTVKVVGSPKDALPPQIYSPNDRVVYEKEPGEELVIPCKVYFSFIMDSHNEV WWTIDGKKPDDVTVDITINESVSYSSTEDETRTQILSIKKVTPEDLRRNYVCHARNTKGEAEQAAKVKQK VIPPRYTVELACGFGATVFLVVVLIVVYHVYWLEMVLFYRAHFGTDETILDGKEYDIYVSYARNVEEEEF VLLTLRGVLENEFGYKLCIFDRDSLPGGNTVEAVFDFIQRSRRMIVVLSPDYVTEKSISMLEFKLGVMCQ NSIATKLIVVEYRPLEQPHPGIMQLKESVSFVSWKGEKSKHSGSKFWKALRLALPLRSLSASSGWNESCS SQSDISLDHVQRRSRLKEPPELRSSERVSGAEPAPGTMSKHRGKPSAACRCCVTYCEGESHLRSKSRAEM HTHPQWETHLCKPPLQESESQWIQNGTRPEPAPQISALALRHFTDLSNNNDFYIL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001159318 |
| ORF Size | 2055 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001159318.1, NP_001152790.1 |
| RefSeq Size | 4320 bp |
| RefSeq ORF | 2058 bp |
| Locus ID | 16180 |
| UniProt ID | Q61730 |
| MW | 79.1 kDa |
| Gene Summary | Coreceptor for IL1RL2 in the IL-36 signaling system. Coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Secreted forms (isoforms 2 and 3) associate with secreted ligand-bound IL1R2 and increase the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. Coreceptor for IL1RL1 in the IL-33 signaling system. Can bidirectionally induce pre- and postsynaptic differentiation of neurons by trans-synaptically binding to PTPRD (PubMed:25908590). May play a role in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG224562 | Il1rap (tGFP-tagged) - Mouse interleukin 1 receptor accessory protein (Il1rap) transcript variant 4, (10ug) |
CNY 7,510.00 |
|
| MR224562L3 | Lenti ORF clone of Il1rap (Myc-DDK-tagged) - Mouse interleukin 1 receptor accessory protein (Il1rap), transcript variant 4 |
CNY 8,740.00 |
|
| MR224562L4 | Lenti ORF clone of Il1rap (mGFP-tagged) - Mouse interleukin 1 receptor accessory protein (Il1rap), transcript variant 4 |
CNY 8,740.00 |
