Trpc5 (NM_009428) Mouse Tagged ORF Clone

CAT＃: MR226053

  • TrueORF®

Trpc5 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily C, member 5 (Trpc5)

ORF Plasmid: DDK tGFP

Datasheet
SDS
Plasmid Map

  "NM_009428" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 7,130.00

货期*
现货
规格
    • 10 ug
Cited in 2 publications.

Product images

经常一起买 (4)
PS100001
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00

添加到购物车
CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售
PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

添加到购物车
TA592569S
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

添加到购物车

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms CCE2; TRP-5; TRP5; Trrp5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR226053 representing NM_009428
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCAGCTGTACTACAAGAAGGTCAATTACTCACCATACAGAGATCGCATCCCCCTCCAAATCGTGA
GGGCTGAGACTGAGCTGTCTGCTGAGGAGAAAGCCTTCCTTAGTGCTGTGGAGAAGGGGGACTATGCCAC
AGTGAAGCAGGCACTGCAGGAGGCCGAGATCTACTACAATGTCAACATCAACTGCATGGATCCTTTGGGC
AGGAGCGCCCTGCTCATTGCCATTGAGAATGAGAACTTGGAGATCATGGAGCTACTGCTGAACCACAGTG
TGTATGTGGGCGATGCATTACTCTACGCCATCCGCAAGGAGGTGGTAGGAGCTGTGGAACTTCTGCTTAG
CTACAGGAAGCCCAGTGGAGAGAAGCAGGTTCCCACGCTGATGATGGACACCCAGTTCTCTGAGTTCACA
CCAGACATCACTCCCATTATGTTGGCAGCCCACACCAACAACTATGAAATCATCAAATTGCTTGTCCAAA
AGAGGGTCACTATCCCAAGGCCCCACCAGATCCGCTGCAACTGTGTGGAGTGTGTATCCAGTTCGGAGGT
AGATAGTCTACGTCATTCTAGGTCCCGACTGAATATATACAAGGCTCTGGCAAGCCCCTCGCTCATTGCC
TTATCAAGCGAGGACCCCATCCTCACGGCCTTTCGGCTGGGCTGGGAACTCAAGGAGCTGAGCAAAGTGG
AAAATGAGTTCAAGGCTGAGTACGAGGAACTTTCCCAGCAATGTAAGCTCTTTGCCAAGGATCTCTTGGA
CCAAGCTCGGAGCTCCAGAGAACTGGAGATCATCCTCAACCATCGAGATGACCACAGTGAAGAACTTGAC
CCACAGAAGTATCATGACCTGGCCAAGCTGAAGGTGGCAATCAAATATCACCAGAAAGAGTTTGTCGCTC
AGCCAAACTGCCAACAATTGCTAGCCACCTTGTGGTACGATGGCTTCCCTGGATGGCGGAGAAAACACTG
GGTAGTCAAGCTTCTGACCTGCATGACCATTGGATTCCTGTTTCCTATGCTGTCCATAGCCTATCTGATC
TCACCCAGAAGCAACCTTGGGCTTTTCATCAAGAAACCCTTTATTAAGTTCATCTGCCACACAGCATCCT
ATCTGACCTTCCTCTTCATGCTTCTCCTGGCTTCTCAACATATCGTCAGAACAGACCTCCATGTTCAAGG
ACCTCCCCCAACTGTTGTGGAATGGATGATACTGCCTTGGGTTCTAGGTTTCATTTGGGGGGAGATAAAG
GAAATGTGGGATGGTGGATTCACGGAATACATCCATGATTGGTGGAACCTGATGGATTTTGCAATGAACT
CCCTCTACCTGGCAACTATTTCCTTGAAGATCGTGGCCTATGTCAAGTATAATGGTTCTCGTCCAAGGGA
AGAATGGGAAATGTGGCACCCGACTCTGATTGCAGAAGCACTCTTCGCAATATCCAACATTTTAAGTTCC
TTGCGTCTCATATCCTTGTTCACAGCCAACTCCCATTTAGGGCCTCTGCAGATCTCTTTGGGACGAATGC
TGCTTGATATACTCAAATTTCTCTTTATCTACTGCCTAGTACTACTGGCTTTTGCCAACGGACTGAACCA
GCTTTACTTTTACTATGAGACCAGAGCTATTGATGAACCTAACAACTGCAAGGGGATCCGATGTGAAAAA
CAGAACAACGCCTTCTCCACGCTCTTTGAAACCCTTCAGTCGCTCTTCTGGTCTGTCTTTGGCCTCCTAA
ATCTCTATGTTACCAATGTGAAGGCCCGACATGAGTTCACTGAGTTTGTGGGAGCTACGATGTTTGGGAC
ATACAACGTCATCTCCCTGGTAGTGCTGCTGAATATGCTCATCGCCATGATGAACAACTCCTACCAGCTC
ATTGCCGATCATGCTGATATTGAGTGGAAGTTTGCGAGAACAAAGCTCTGGATGAGTTACTTTGATGAAG
GTGGCACCTTACCACCTCCTTTCAACATTATTCCCAGCCCCAAATCATTTCTCTATCTTGGCAACTGGTT
CAACAACACCTTCTGTCCCAAAAGAGACCCTGATGGAAGACGAAGGAGGCACAACTTGAGAAGCTTCACA
GAACGTCATGCTGACAGCCTGATACAAAATCAACATTATCAGGAAGTTATCAGAAATTTAGTCAAGAGAT
ATGTGGCTGCAATGATAAGAAACTCCAAAACAAATGAGGGGCTAACAGAAGAAAATTTTAAGGAATTAAA
GCAGGACATTTCCAGCTTTCGATATGAAGTGCTTGACCTCTTGGGAAACAGGAAACATCCAAGAAGAAGC
CTCTCCACCAGCAGTGCTGATTTCTCTCAAAGGGATGATACCAATGACGGCAGTGGTGGGGCTCGGGCCA
AGTCTAAGAGTGTCTCTTTCAATGTAGGCTGCAAGAAAAAGGCCTGTCACGGTGCACCGCTCATCAGAAC
CGTGCCAAGAGCTAGTGGCGCCCAAGGAAAGCCAAAATCCGAGTCGTCGAGCAAACGGTCCTTCATGGGT
CCGTCTTTCAAGAAATTGGGTCTCTTCTTCTCCAAGTTCAATGGTCAAACATCTGAACCTACTTCGGAGC
CAATGTACACAATCTCTGATGGCATCGCACAGCAGCATTGTATGTGGCAGGACATCCGCTATTCTCAGAT
GGAGAAAGGGAAGGCAGAGGCCTGTTCTCAAAGTCAGATGAACCTTGGCGAGGTAGAGCTAGGTGAAATC
CGGGGGGCTGCTGCTCGGAGCAGTGAGTGCCCCCTAGCCTGTTCCAGCTCCCTTCACTGTGCATCCGGCA
TCTGCTCCTCAAATTCGAAACTTTTGGACTCGTCAGAGGACGTATTTGAAACTTGGGGAGAGGCTTGTGA
CTTGCTCATGCACAAATGGGGTGATGGACAGGAAGAACAAGTTACAACTCGGCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>MR226053 representing NM_009428
Red=Cloning site Green=Tags(s)

MAQLYYKKVNYSPYRDRIPLQIVRAETELSAEEKAFLSAVEKGDYATVKQALQEAEIYYNVNINCMDPLG
RSALLIAIENENLEIMELLLNHSVYVGDALLYAIRKEVVGAVELLLSYRKPSGEKQVPTLMMDTQFSEFT
PDITPIMLAAHTNNYEIIKLLVQKRVTIPRPHQIRCNCVECVSSSEVDSLRHSRSRLNIYKALASPSLIA
LSSEDPILTAFRLGWELKELSKVENEFKAEYEELSQQCKLFAKDLLDQARSSRELEIILNHRDDHSEELD
PQKYHDLAKLKVAIKYHQKEFVAQPNCQQLLATLWYDGFPGWRRKHWVVKLLTCMTIGFLFPMLSIAYLI
SPRSNLGLFIKKPFIKFICHTASYLTFLFMLLLASQHIVRTDLHVQGPPPTVVEWMILPWVLGFIWGEIK
EMWDGGFTEYIHDWWNLMDFAMNSLYLATISLKIVAYVKYNGSRPREEWEMWHPTLIAEALFAISNILSS
LRLISLFTANSHLGPLQISLGRMLLDILKFLFIYCLVLLAFANGLNQLYFYYETRAIDEPNNCKGIRCEK
QNNAFSTLFETLQSLFWSVFGLLNLYVTNVKARHEFTEFVGATMFGTYNVISLVVLLNMLIAMMNNSYQL
IADHADIEWKFARTKLWMSYFDEGGTLPPPFNIIPSPKSFLYLGNWFNNTFCPKRDPDGRRRRHNLRSFT
ERHADSLIQNQHYQEVIRNLVKRYVAAMIRNSKTNEGLTEENFKELKQDISSFRYEVLDLLGNRKHPRRS
LSTSSADFSQRDDTNDGSGGARAKSKSVSFNVGCKKKACHGAPLIRTVPRASGAQGKPKSESSSKRSFMG
PSFKKLGLFFSKFNGQTSEPTSEPMYTISDGIAQQHCMWQDIRYSQMEKGKAEACSQSQMNLGEVELGEI
RGAAARSSECPLACSSSLHCASGICSSNSKLLDSSEDVFETWGEACDLLMHKWGDGQEEQVTTRL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_009428
ORF Size 2925 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_009428.3
RefSeq Size 4158 bp
RefSeq ORF 2928 bp
Locus ID 22067
UniProt ID Q9QX29
MW 111.9 kDa
Gene Summary Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Has also been shown to be calcium-selective. May also be activated by intracellular calcium store depletion.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (2)

The use of this cDNA Clones has been cited in the following citations:
Transient receptor potential canonical 5 mediates inflammatory mechanical and spontaneous pain in mice ,Sadler, KE;Moehring, F;Shiers, SI;Laskowski, LJ;Mikesell, AR;Plautz, ZR;Brezinski, AN;Mecca, CM;Dussor, G;Price, TJ;McCorvy, JD;Stucky, CL;, Science translational medicine ,PubMed ID 34039739 [TRPC5]
Transient receptor potential canonical 5 (TRPC5) mediates inflammatory mechanical pain ,Sadler, K;Moehring, F;Shiers, S;Laskowski, L;Mikesell, A;Plautz, Z;Mecca, C;Dussor, G;Price, T;McCorvy, J;Stucky, C;, bioRxiv [TRPC5]

Other Versions

SKU Description Size Price
MC222902 Trpc5 (untagged) - Mouse transient receptor potential cation channel, subfamily C, member 5 (Trpc5), (10ug)
    • 10 ug

CNY 7,152.00

MG226053 Trpc5 (tGFP-tagged) - Mouse transient receptor potential cation channel subfamily C member 5 (Trpc5), (10ug)
    • 10 ug

CNY 7,890.00

MR226053L3 Lenti ORF clone of Trpc5 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily C, member 5 (Trpc5)
    • 10 ug

CNY 9,030.00

MR226053L4 Lenti ORF clone of Trpc5 (mGFP-tagged) - Mouse transient receptor potential cation channel, subfamily C, member 5 (Trpc5)
    • 10 ug

CNY 9,030.00

You may also need
plasmid icon Empty Vector
protein iconDDK/Flag Antibody

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Expression Plasmids
Lentiviral Particles
CRISPR
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Proteins
Antibodies
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MR226053L3

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MG226053

Trpc5 (tGFP-tagged) - Mouse transient receptor potential cation channel subfamily C member 5 (Trpc5), (10ug)

    • 10 ug

CNY 7,890.00

MR226053L4

Lenti ORF clone of Trpc5 (mGFP-tagged) - Mouse transient receptor potential cation channel, subfamily C, member 5 (Trpc5)

    • 10 ug

CNY 9,030.00

MR226053L3V

Lenti ORF particles, Trpc5 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily C, member 5 (Trpc5), 200ul, >10^7 TU/mL

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MC222902

Trpc5 (untagged) - Mouse transient receptor potential cation channel, subfamily C, member 5 (Trpc5), (10ug)

    • 10 ug

CNY 7,152.00

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