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Ctnnb1 (NM_001165902) Mouse Tagged ORF Clone
CAT＃: MR227484
- TrueORF®
Ctnnb1 (Myc-DDK-tagged) - Mouse catenin (cadherin associated protein), beta 1 (Ctnnb1), transcript variant 2
ORF Plasmid: tGFP
"NM_001165902" in other vectors (4)
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CNY 5,720.00
CNY 7,790.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Bfc; Cat; Catnb; Mesc |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227484 representing NM_001165902
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTACTCAAGCTGACCTGATGGAGTTGGACATGGCCATGGAGCCGGACAGAAAAGCTGCTGTCAGCC ACTGGCAGCAGCAGTCTTACTTGGATTCTGGAATCCATTCTGGTGCCACCACCACAGCTCCTTCCCTGAG TGGCAAGGGCAACCCTGAGGAAGAAGATGTTGACACCTCCCAAGTCCTTTATGAATGGGAGCAAGGCTTT TCCCAGTCCTTCACGCAAGAGCAAGTAGCTGATATTGACGGGCAGTATGCAATGACTAGGGCTCAGAGGG TCCGAGCTGCCATGTTCCCTGAGACGCTAGATGAGGGCATGCAGATCCCATCCACGCAGTTTGACGCTGC TCATCCCACTAATGTCCAGCGCTTGGCTGAACCATCACAGATGTTGAAACATGCAGTTGTCAATTTGATT AACTATCAGGATGACGCGGAACTTGCCACACGTGCAATTCCTGAGCTGACAAAACTGCTAAACGATGAGG ACCAGGTGGTAGTTAATAAAGCTGCTGTTATGGTCCATCAGCTTTCCAAAAAGGAAGCTTCCAGACATGC CATCATGCGCTCCCCTCAGATGGTGTCTGCCATTGTACGCACCATGCAGAATACAAATGATGTAGAGACA GCTCGTTGTACTGCTGGGACTCTGCACAACCTTTCTCACCACCGCGAGGGCTTGCTGGCCATCTTTAAGT CTGGTGGCATCCCAGCGCTGGTGAAAATGCTTGGGTCACCAGTGGATTCTGTACTGTTCTACGCCATCAC GACACTGCATAATCTCCTGCTCCATCAGGAAGGAGCTAAAATGGCAGTGCGCCTAGCTGGTGGACTGCAG AAAATGGTTGCTTTGCTCAACAAAACAAACGTGAAATTCTTGGCTATTACAACAGACTGCCTTCAGATCT TAGCTTATGGCAATCAAGAGAGCAAGCTCATCATTCTGGCCAGTGGTGGACCCCAAGCCTTAGTAAACAT AATGAGGACCTACACTTATGAGAAGCTTCTGTGGACCACAAGCAGAGTGCTGAAGGTGCTGTCTGTCTGC TCTAGCAACAAGCCGGCCATTGTAGAAGCTGGTGGGATGCAGGCACTGGGGCTTCATCTGACAGACCCAA GTCAGCGACTTGTTCAAAACTGTCTTTGGACTCTCAGAAACCTTTCAGATGCAGCGACTAAGCAGGAAGG GATGGAAGGCCTCCTTGGGACTCTAGTGCAGCTTCTGGGTTCCGATGATATAAATGTGGTCACCTGTGCA GCTGGAATTCTCTCTAACCTCACTTGCAATAATTACAAAAACAAGATGATGGTGTGCCAAGTGGGTGGCA TAGAGGCTCTTGTACGCACCGTCCTTCGTGCTGGTGACAGGGAAGACATCACTGAGCCTGCCATCTGTGC TCTTCGTCATCTGACCAGCCGGCATCAGGAAGCCGAGATGGCCCAGAATGCCGTTCGCCTTCATTATGGA CTGCCTGTTGTGGTTAAACTCCTGCACCCACCATCCCACTGGCCTCTGATAAAGGCAACTGTTGGATTGA TTCGAAACCTTGCCCTTTGCCCAGCAAATCATGCGCCTTTGCGGGAACAGGGTGCTATTCCACGACTAGT TCAGCTGCTTGTACGAGCACATCAGGACACCCAACGGCGCACCTCCATGGGTGGAACGCAGCAGCAGTTT GTGGAGGGCGTGCGCATGGAGGAGATAGTAGAAGGGTGTACTGGAGCTCTCCACATCCTTGCTCGGGACG TTCACAACCGGATTGTAATCCGAGGACTCAATACCATTCCATTGTTTGTGCAGTTGCTTTATTCTCCCAT TGAAAATATCCAAAGAGTAGCTGCAGGGGTCCTCTGTGAACTTGCTCAGGACAAGGAGGCTGCAGAGGCC ATTGAAGCTGAGGGAGCCACAGCTCCCCTGACAGAGTTACTCCACTCCAGGAATGAAGGCGTGGCAACAT ACGCAGCTGCTGTCCTATTCCGAATGTCTGAGGACAAGCCACAGGATTACAAGAAGCGGCTTTCAGTCGA GCTGACCAGTTCCCTCTTCAGGACAGAGCCAATGGCTTGGAATGAGACTGCAGATCTTGGACTGGACATT GGTGCCCAGGGAGAAGCCCTTGGATATCGCCAGGATGATCCCAGCTACCGTTCTTTTCACTCTGGTGGAT ACGGCCAGGATGCCTTGGGGATGGACCCTATGATGGAGCATGAGATGGGTGGCCACCACCCTGGTGCTGA CTATCCAGTTGATGGGCTGCCTGATCTGGGACACGCCCAGGACCTCATGGATGGGCTGCCCCCAGGTGAT AGCAATCAGCTGGCCTGGTTTGATACTGACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227484 representing NM_001165902
Red=Cloning site Green=Tags(s) MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGF SQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLI NYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVET ARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQ KMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVC SSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCA AGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYG LPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQF VEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEA IEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDI GAQGEALGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGD SNQLAWFDTDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001165902 |
| ORF Size | 2343 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001165902.1, NP_001159374.1 |
| RefSeq Size | 3440 bp |
| RefSeq ORF | 2346 bp |
| Locus ID | 12387 |
| UniProt ID | Q02248 |
| MW | 85.5 kDa |
| Gene Summary | This gene encodes not only an important cytoplasmic component of the classical cadherin adhesion complex that forms the adherens junction in epithelia and mediates cell-cell adhesion in many other tissues but also a key signaling molecule in the canonical Wnt signaling pathway that controls cell growth and differentiation during both normal development and tumorigenesis. The gene product contains a central armadillo-repeat containing domain through which it binds the cytoplasmic tail of classical cadherins; meanwhile, it also binds alpha-catenin, which further links the cadherin complex to the actin cytoskeleton either directly or indirectly. Beta-catenin is therefore necessary for the adhesive function of classical cadherins. Another key function of this protein is to mediate the canonical Wnt signaling pathway and regulate gene transcription. Without Wnt signal, cytoplasmic beta-catenin that is not associated with the cadherin complex is quickly phosphorylated at the N-terminal Ser/Thr residues by the so called degradation complex containing axin, adenomatous polyposis coli (APC), casein kinase I, and GSK3B, then ubiquitylated by beta-TrCP, and degraded by the proteasome. However, in the presence of Wnt signal, the degradation complex is disrupted and the stabilized cytoplasmic beta-catenin translocates into the nucleus, where it binds various transcription factors and, together with these factors, regulates the transcription of many downstream genes. Mutations of this gene have been linked with various types of tumors. Alternatively spliced variants have been found for this gene. [provided by RefSeq, Sep 2009] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221551 | Ctnnb1 (untagged) - Mouse catenin (cadherin associated protein), beta 1 (Ctnnb1), transcript variant 2, (10ug) |
CNY 10,070.00 |
|
| MG227484 | Ctnnb1 (tGFP-tagged) - Mouse catenin (cadherin associated protein) beta 1 (Ctnnb1) transcript variant 2, (10ug) |
CNY 8,550.00 |
|
| MR227484L3 | Lenti ORF clone of Ctnnb1 (Myc-DDK-tagged) - Mouse catenin (cadherin associated protein), beta 1 (Ctnnb1), transcript variant 2 |
CNY 9,690.00 |
|
| MR227484L4 | Lenti ORF clone of Ctnnb1 (mGFP-tagged) - Mouse catenin (cadherin associated protein), beta 1 (Ctnnb1), transcript variant 2 |
CNY 9,690.00 |
