Lef1 (NM_001276403) Mouse Tagged ORF Clone
CAT#: MR229286
- TrueORF®
Lef1 (myc-DDK-tagged) - Mouse lymphoid enhancer binding factor 1 (Lef1), transcript variant 3
"NM_001276403" in other vectors (1)
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CNY 3,330.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 3000002B05; AI451430; Lef-1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR229286 representing NM_001276403
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGGGTGGTCAGACAAGCCCCGTCCTCTCAGGAGCCCTACCACGACAAGGCCAGAGAACACCCTG ATGAAGGAAAGCATCCAGACGGAGGCCTGTACAACAAGGGACCCTCCTACTCCAGTTACTCTGGCTACAT AATGATGCCCAATATGAACAGCGACCCGTACATGTCAAATGGGTCCCTTTCTCCACCCATCCCGAGGACA TCAAATAAAGTGCCCGTGGTGCAGCCCTCTCACGCGGTCCACCCGCTCACCCCCCTCATCACCTACAGCG ACGAGCACTTTTCTCCGGGATCCCACCCGTCACACATCCCGTCAGATGTCAACTCCAAGCAAGGCATGTC CAGACACCCTCCAGCTCCTGAAATCCCCACCTTCTACCCCCTGTCTCCGGGCGGCGTTGGACAGATCACC CCACCCATTGGCTGGCAAGGTCAGCCTGTTTATCCCATCACGGGTGGATTCAGGCAACCCTACCCATCCT CACTGTCAGGCGACACTTCCATGTCCAGGTTTTCCCATCATATGATTCCTGGTCCCCCTGGCCCCCACAC AACTGGCATCCCTCATCCAGCTATTGTAACACCTCAGGTCAAACAGGAGCACCCCCACACGGACAGTGAC CTAATGCACGTGAAGCCTCAACACGAACAGAGAAAGGAGCAGGAGCCCAAAAGACCTCATATTAAGAAGC CTCTGAATGCTTTCATGTTATATATGAAAGAAATGAGAGCGAATGTCGTAGCTGAGTGCACGCTAAAGGA GAGTGCAGCTATCAACCAGATCCTGGGCAGAAGATGGCACGCCCTCTCCCGGGAAGAGCAGGCCAAATAC TATGAACTAGCACGGAAAGAGAGACAGCTACACATGCAGCTTTATCCAGGCTGGTCAGCGCGAGACAATT ATGGCAAGAAGAAGAAGAGGAAGAGAGAGAAGCTACAGGAGTCGACTTCAGGTACAGGTCCCAGAATGAC AGCTGCCTACATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR229286 representing NM_001276403
Red=Cloning site Green=Tags(s) MAGVVRQAPSSQEPYHDKAREHPDEGKHPDGGLYNKGPSYSSYSGYIMMPNMNSDPYMSNGSLSPPIPRT SNKVPVVQPSHAVHPLTPLITYSDEHFSPGSHPSHIPSDVNSKQGMSRHPPAPEIPTFYPLSPGGVGQIT PPIGWQGQPVYPITGGFRQPYPSSLSGDTSMSRFSHHMIPGPPGPHTTGIPHPAIVTPQVKQEHPHTDSD LMHVKPQHEQRKEQEPKRPHIKKPLNAFMLYMKEMRANVVAECTLKESAAINQILGRRWHALSREEQAKY YELARKERQLHMQLYPGWSARDNYGKKKKRKREKLQESTSGTGPRMTAAYI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001276403 |
ORF Size | 993 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001276403.1, NP_001263332.1 |
RefSeq Size | 2305 bp |
RefSeq ORF | 996 bp |
Locus ID | 16842 |
MW | 37.5 kDa |
Gene Summary | Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1 (By similarity). Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIASG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1.[UniProtKB/Swiss-Prot Function] |
Documents
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC227075 | Lef1 (untagged) - Mouse lymphoid enhancer binding factor 1 (Lef1), transcript variant 3 |
CNY 3,230.00 |