Plcd1 (NM_001293648) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AW212592; C79986
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231135 representing NM_001293648 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTGTCTAGGGGTCCGGAGTTGCAGACGGGGCAGCGTGAACCGCTCCCGGGAGCTCTACCTGCAGG AGCAGAGTCGCAAAGTAGCGGCGCTCAATGGACAGAGACTGGGGCTGCAGGATGACCCGGACCTTCAGGC CCTCCTGAAGGGCAGCCAGCTTCTGAAGGTGAAGTCCAGCTCGTGGCGTAGAGAACGCTTCTACAAGCTA CAGGAGGACTGCAAGACCATCTGGCAGGAATCTCGCAAGGTCATGAGGTCCCCGGAGTCGCAGCTGTTCT CCATCGAGGACATTCAGGAGGTGCGGATGGGACACCGCACAGAAGGCCTGGAGAAATTTGCCAGAGACAT ACCCGAGGACCGATGCTTCTCCATCGTCTTCAAGGACCAGCGCAATACCCTAGACCTCATCGCCCCGTCT CCAGCTGACGTCCAGCATTGGGTGCAGGGCCTGCGCAAGATCATCGACCGCTCGGGCTCCATGGACCAGC GGCAGAAGCTGCAGCACTGGATTCACTCCTGCTTGCGAAAGGCTGATAAAAACAAGGATAACAAGATGAA CTTCAAGGAGGTGAAGGACTTCCTGAAGGAGCTCAACGTACAGGTGGATGACAGCTACGCCAGGAAGATC TTCAGGGAATGTGACCACTCCCAGACAGACTCGCTGGAGGATGAGGAGATTGAGACCTTCTACAGGATGC TGACCCAGCGGGCAGAGATCGACCGTGCCTTTGCAGAGGCCGCAGGTTCTGCGGAGACCCTGTCGGTGGA GAAGCTAGTGACGTTCCTGCAGCATCAGCAGCGGGAGGAGGAGGCGGGGCCAGCCTTGGCCCTCTCTCTC ATTGAGCGCTACGAGCCCAGTGAGACTGCCAAAGCCCAGCGGCAGATGACCAAGGATGGCTTCCTCATGT ACCTGCTGTCTGCTGACGGTAACGCCTTCAGCCTGGCGCACCGCCGTGTCTACCAGGACATGAACCAGCC GCTCAGCCACTACTTAGTGTCTTCTTCCCACAACACCTACCTACTGGAGGACCAGCTCACAGGGCCCAGC AGCACGGAGGCCTACATCCGGGCTCTGTGCAAAGGCTGCCGGTGTCTGGAGCTGGACTGCTGGGACGGCC CCAACCAGGAACCCATCATCTACCATGGCTACACTTTTACCTCTAAGATACTTTTCTGCGACGTGCTCAG GGCCATCCGGGACTACGCCTTCAAGGCATCCCCCTACCCAGTTATCCTGTCCCTGGAGAACCACTGCAGC CTGGAGCAACAACGAGTCATGGCTCATCACCTGAGGGCCATCCTGGGGCCCATGCTGTTGGACCAGCCGC TGGATGGGGTTACCACGAGCCTGCCTTCGCCTGAGCAACTGAAGGAAAAGATTCTGTTGAAAGGGAAGAA ACTGGGCGGGCTGCTACCTGCTGGAGGGGAAAACGGGCCTGAAGCCACCGATGTGTCTGACGAAGATGAG GCTGCTGAAATGGAAGATGAGGCCGTGCGCAGCCAAGTGCAACACAAGCCCAAGGAGGATAAGCTAAAGC TGGTGCCGGAGCTCTCCGACATGGTCATTTACTGCAAGAGTGTCCACTTTGGTGGCTTCTCCAGTCCTAG CACCTCTGGGCAGGCATTCTATGAGATGGCTTCCTTCTCCGAGAGCCGTGCCCTGCGGCTGCTGCAAGAA TCAGGAAATAGTTTTGTCCGCCATAACGTGGGCCATCTGAGCAGGATCTACCCAGCTGGGTGGAGGACAG ATTCCTCCAACTACAGTCCCGTGGAGATGTGGAATGGAGGCTGCCAGATTGTGGCTCTGAACTTCCAAAC CCCTGGGCCAGAAATGGATGTTTACCTTGGCTGCTTCCAGGACAATGGAGGTTGTGGGTATGTGCTGAAA CCGGCCTTCCTGAGAGACCCTGACACCACCTTCAACTCACGGGCCCTGACTCAGGGGCCCTGGTGGGCCC CGAAGAAGCTCCGAGTCTGGATCATCTCTGGACAGCAGCTGCCGAAGGTCAATAAGAATAAGAATTCTAT CGTGGACCCCAAGGTGATAGTGGAGATCCACGGTGTGGGCCAGGATGTGGCCAGCCGTCAGACAGCAGTT ATTACCAATAATGGTTTCAACCCACGGTGGGACACGGAGTTTGAGTTTGTGGTAGCAGTGCCTGACCTTG CCCTTGTACGTTTCATGGTAGAGGATTATGACTCCTCCTCTAAGAACGACTTTATCGGCCAGAGTACTAT CCCCTGGAACAGCCTCAAGCAGGGGTACCGCCATGTCCACCTCTTGTCTAAGAATGGAGACCTGCATCCA TCAGCCACTCTCTTTGTGAAGATCTCCATCCAGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231135 representing NM_001293648 Red=Cloning site Green=Tags(s) MQCLGVRSCRRGSVNRSRELYLQEQSRKVAALNGQRLGLQDDPDLQALLKGSQLLKVKSSSWRRERFYKL QEDCKTIWQESRKVMRSPESQLFSIEDIQEVRMGHRTEGLEKFARDIPEDRCFSIVFKDQRNTLDLIAPS PADVQHWVQGLRKIIDRSGSMDQRQKLQHWIHSCLRKADKNKDNKMNFKEVKDFLKELNVQVDDSYARKI FRECDHSQTDSLEDEEIETFYRMLTQRAEIDRAFAEAAGSAETLSVEKLVTFLQHQQREEEAGPALALSL IERYEPSETAKAQRQMTKDGFLMYLLSADGNAFSLAHRRVYQDMNQPLSHYLVSSSHNTYLLEDQLTGPS STEAYIRALCKGCRCLELDCWDGPNQEPIIYHGYTFTSKILFCDVLRAIRDYAFKASPYPVILSLENHCS LEQQRVMAHHLRAILGPMLLDQPLDGVTTSLPSPEQLKEKILLKGKKLGGLLPAGGENGPEATDVSDEDE AAEMEDEAVRSQVQHKPKEDKLKLVPELSDMVIYCKSVHFGGFSSPSTSGQAFYEMASFSESRALRLLQE SGNSFVRHNVGHLSRIYPAGWRTDSSNYSPVEMWNGGCQIVALNFQTPGPEMDVYLGCFQDNGGCGYVLK PAFLRDPDTTFNSRALTQGPWWAPKKLRVWIISGQQLPKVNKNKNSIVDPKVIVEIHGVGQDVASRQTAV ITNNGFNPRWDTEFEFVVAVPDLALVRFMVEDYDSSSKNDFIGQSTIPWNSLKQGYRHVHLLSKNGDLHP SATLFVKISIQD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001293648
ORF Size	2346 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001293648.1, NP_001280577.1
RefSeq Size	2706 bp
RefSeq ORF	2349 bp
Locus ID	18799
UniProt ID	Q8R3B1
MW	89.3 kDa
Gene Summary	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Essential for trophoblast and placental development.[UniProtKB/Swiss-Prot Function]

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