Sorcs1 (NM_001290356) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	mSorCS; Sorcs
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231625 representing NM_001290356 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAAAGTTGGCGCTGGAGACGGCTCCTCGGCCGGGCTGAGCGCGCTCCTTGCAGGAGCGGGGCTTC TGATGCTCTTAGCCCCCGGCGTCTGCAGCAGCCTCTCTTGCTGCCCTCCGCAGCACCCTAGCTCGACCCC ACGCCGGACCCTTACCCCAAGAGGCTTTCCTCACCCGGGACCTCTGGGTCGGGCTCCTGCCACGCCCCCG CCCCTCTTCATGAGACCCCTGTTCGCAGTGGCCCCCGGGGACCGTGCGCTGTTTCTGGAGCGAGCTGGGG GCAGCAGGGTGTCAGTGGCGACCGCTGCACGCTCTGGCCGTAGGAGACGGAGTGGAACGGAACCCGAGAA GATTGAACCCGGAGAGGGTGCGAGTCGGAGCCGCCGGGACATGCTAAAGGATGGAGGGCAGCAGGGGCTT GGGACTGGCGCACGGGACCCGGACAAAGCCACCCGCTTCCGGATGGAGGAGCTGAGACTGACCAGCACCA CATTTGCTCTGACAGGAGACTCAGCACACAACCAAGCTATGGTCCACTGGTCTGGCCACAACAGCAGCGT GATTCTCATTTTGACAAAGCTCTATGACTATAACCTGGGGAGTATCACCGAGAGCTCGCTTTGGAGGTCA ACCGATTATGGGACTACCTATGAAAAGTTGAATGATAAAGTGGGGTTGAAGACAATTTTGAGCTATCTCT ATGTGTGTCCAACCAACAAGCGTAAGATTATGTTACTCACAGACCCGGAGATTGAGAGCAGTTTGTTGAT CAGCTCAGATGAAGGGGCAACCTATCAAAAGTACCGGCTCAACTTTTACATTCAGAGTTTGCTTTTCCAT CCGAAGCAAGAAGACTGGATCCTGGCATACAGTCAAGACCAAAAGTTATACAGCTCTGCTGAGTTTGGCC GAAGATGGCAGCTTATCCAGGAATCAGTGGTACCAAATAGGTTCTACTGGTCTGTGATGGGGTCGAGCAA AGAACCAGACCTTGTGCATCTCGAGGCCAGGACTGTGGATGGTCACTCAATATACCTAACTTGTCGGATG CAAAACTGCACTGAAGCCAACAGAAATAAGCCTTTCCCAGGTTACATCGACCCGGACTCTTTGATTGTTC AGGACGATTATGTGTTTGTTCAGCTGACATCAGGAGGAAGACCACATTACTATGTGTCCTACCGAAGAAG CCCGTTTGCACAAATGAAGCTTCCGAAATACGCTTTGCCCAAGGACATGCACGTCATTAGCACAGACGAG AACCAAGTGTTTGCAGCCGTTCAAGAATGGAACCAGAACGACACCTACAACCTCTACATCTCAGACACAC GAGGAGTCTACTTCACACTGGCCTTGGAGAATGTGCAGAGCAGCAGAGGCCCTGAAGGCAACGTCATGAT TGACCTTTATGAGGTAGCAGGGATAAAGGGAATGTTCTTGGCTAACAAGAAGATTGACAACCAAGTGAAG ACTTTCATCACTTACAACAAAGGCAGAGACTGGCGGTTGCTGCAGGCTCCAGATGCAGATCTAAGGGGGG ACCCTGTGCACTGTTTGCTGCCCTACTGTTCGCTACACCTTCACCTGAAGGTTTCTGAGAATCCCTACAC ATCCGGGATCATTGCCAGCAGAGACACAGCCCCAAGTATTATAGTTGCATCAGGTAACATAGGCTCTGAG TTGTCAGACAGTGACATCAGCATGTTTGTATCTTCAGATGCAGGGAACACTTGGAGGCAGATTTTTGAAG AAGAGCACAGCATTTTGTACCTTGACCAAGGTGGAGTCCTTGTTGCTATGAAACACACATCTCTCCCAAT TCGACACCTTTGGTTGAGTTTTGATGAAGGGAGATCTTGGAGCAAATACAGCTTTACATCCATTCCACTC TTTGTGGATGGAGTCCTGGGGGAGCCTGGAGAAGAGACACTAATCATGACAGTGTTTGGACACTTCAGTC ATCGTTCCGAATGGCAGCTGGTCAAAGTGGACTACAAGTCCATTTTTGATAGACGCTGTGCTGAAGAAGA CTACAGACCCTGGCAGTTGCATAGTCAGGGGGAAGCATGCATCATGGGAGCCAAGAGGATATACAAGAAG AGAAAGTCTGAGCGCAAGTGTATGCAAGGAAAGTATGCGGGAGCAATGGAGTCTGAGCCTTGTGTTTGTA CAGAGGCAGACTTTGACTGTGACTATGGCTATGAGAGACATAGTAATGGACAATGCCTGCCAGCATTTTG GTTCAACCCATCCTCACTGTCAAAAGACTGCAGCTTGGGACAGAGTTACCTCAACAGCACTGGGTATAGG AAGGTGGTCTCCAATAATTGTACTGATGGAGTGAGAGAGCAGTATACCGCCAAACCACAGAAGTGTCCAG GGAAGGCCCCTCGTGGGCTGCGGATTGTCACTGCAGATGGGAAACTGACAGCAGAGCAGGGCCACAATGT CACTCTCATGGTGCAGCTGGAAGAGGGTGATGTGCAGCGGACACTCATCCAAGTGGACTTCGGAGATGGC ATTGCAGTATCGTATGTCAACCTCAGCTCTATGGAAGACGGTATCAAGCACGTATATCAGAATGTGGGCA TTTTCCGAGTGACCGTGCAGGTGGACAACAGTCTGGGGTCTGACAGCGCCGTCCTGTACTTACATGTAAC TTGTCCCCTGGAGCATGTGCACCTGTCTCTTCCTTTTGTCACAACAAAGAACAAAGAGGTCAATGCAACT GCAGTGCTTTGGCCCAGCCAAGTGGGCACTCTCACTTACGTGTGGTGGTATGGGAACAACACAGAGCCCC TGATCACCTTGGAGGGAAGCATATCGTTCAAGTTTACTTCTGAAGGAATGAATACTATCACAGTCCAAGT CTCTGCTGGGAACGCCATACTGCAAGACACAAAGACCATTGCGGTATATGAGGAATTTCGGTCTCTTCGC TTGGCCTTTTCTCCGAACTTGGATGACTACAACCCTGACATCCCGGAGTGGAGGAGGGACATCAGCCGAG TCATCAAAAAGTCTCTGGTGGAAGCCACAGGGATTCCCAGCCAGCATATCCTGGTGGCGGTGTTACCTGG CTTACCCACTGCTGCAGAGCTCTTTGTTTTGCCCTACCAAGATGGAACCAGAGAAAACAAAAGGTCGCCT GAGGACCTGGAGCAGATATCTGAAGTTCTAATCCACAAACTCAACCAAAACTTGGTGCACTTCGAGCTGA AGCCTGGCGTCCAAGTTCTTGTCCACGCAGCTCACCTGACAGCAGCTCCACTGGTGGACCTCACTCCTAC CCACAGCGGATCTGCCATGCTGATGTTGCTCTCTGTGGTGTTTGTGGGGCTCGCTGTATTCGTCATCTAC AAGTTTAAAAGGTGTGTGTTTCTTCTTCTGCCTTCCTACCCCCGACCCCCACCTCCCTCCTCTTTCTGCC AGGTTCAGAAGCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231625 representing NM_001290356 Red=Cloning site Green=Tags(s) MGKVGAGDGSSAGLSALLAGAGLLMLLAPGVCSSLSCCPPQHPSSTPRRTLTPRGFPHPGPLGRAPATPP PLFMRPLFAVAPGDRALFLERAGGSRVSVATAARSGRRRRSGTEPEKIEPGEGASRSRRDMLKDGGQQGL GTGARDPDKATRFRMEELRLTSTTFALTGDSAHNQAMVHWSGHNSSVILILTKLYDYNLGSITESSLWRS TDYGTTYEKLNDKVGLKTILSYLYVCPTNKRKIMLLTDPEIESSLLISSDEGATYQKYRLNFYIQSLLFH PKQEDWILAYSQDQKLYSSAEFGRRWQLIQESVVPNRFYWSVMGSSKEPDLVHLEARTVDGHSIYLTCRM QNCTEANRNKPFPGYIDPDSLIVQDDYVFVQLTSGGRPHYYVSYRRSPFAQMKLPKYALPKDMHVISTDE NQVFAAVQEWNQNDTYNLYISDTRGVYFTLALENVQSSRGPEGNVMIDLYEVAGIKGMFLANKKIDNQVK TFITYNKGRDWRLLQAPDADLRGDPVHCLLPYCSLHLHLKVSENPYTSGIIASRDTAPSIIVASGNIGSE LSDSDISMFVSSDAGNTWRQIFEEEHSILYLDQGGVLVAMKHTSLPIRHLWLSFDEGRSWSKYSFTSIPL FVDGVLGEPGEETLIMTVFGHFSHRSEWQLVKVDYKSIFDRRCAEEDYRPWQLHSQGEACIMGAKRIYKK RKSERKCMQGKYAGAMESEPCVCTEADFDCDYGYERHSNGQCLPAFWFNPSSLSKDCSLGQSYLNSTGYR KVVSNNCTDGVREQYTAKPQKCPGKAPRGLRIVTADGKLTAEQGHNVTLMVQLEEGDVQRTLIQVDFGDG IAVSYVNLSSMEDGIKHVYQNVGIFRVTVQVDNSLGSDSAVLYLHVTCPLEHVHLSLPFVTTKNKEVNAT AVLWPSQVGTLTYVWWYGNNTEPLITLEGSISFKFTSEGMNTITVQVSAGNAILQDTKTIAVYEEFRSLR LAFSPNLDDYNPDIPEWRRDISRVIKKSLVEATGIPSQHILVAVLPGLPTAAELFVLPYQDGTRENKRSP EDLEQISEVLIHKLNQNLVHFELKPGVQVLVHAAHLTAAPLVDLTPTHSGSAMLMLLSVVFVGLAVFVIY KFKRCVFLLLPSYPRPPPPSSFCQVQKQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290356
ORF Size	3444 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290356.1, NP_001277285.1
RefSeq Size	3650 bp
RefSeq ORF	3447 bp
Locus ID	58178
MW	128.5 kDa

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