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MCM3 (NM_002388) Human Tagged ORF Clone
CAT＃: RC200208
MCM3 (Myc-DDK-tagged)-Human minichromosome maintenance complex component 3 (MCM3)
ORF Plasmid: tGFP
"NM_002388" in other vectors (4)
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CNY 5,320.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HCC5; P1-MCM3; P1.h; RLFB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200208 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGTACCGTGGTGCTGGACGATGTGGAGCTGCGGGAGGCTCAGAGAGATTACCTGGACTTCCTGG ACGACGAGGAAGACCAGGGAATTTATCAGAGCAAAGTTCGGGAGCTGATCAGTGACAACCAATACCGGCT GATTGTCAATGTGAATGACCTGCGCAGGAAAAACGAGAAGAGGGCTAACCGGCTTCTGAACAATGCCTTT GAGGAGCTGGTTGCCTTCCAGCGGGCCTTAAAGGATTTTGTGGCCTCCATTGATGCTACCTATGCCAAGC AGTATGAGGAGTTCTACGTAGGACTGGAAGGCAGCTTTGGCTCCAAGCACGTCTCCCCGCGGACTCTTAC CTCCTGCTTCCTCAGCTGTGTGGTCTGTGTGGAGGGCATTGTCACTAAATGTTCTCTAGTTCGTCCCAAA GTCGTCCGCAGTGTCCACTACTGTCCTGCTACTAAGAAGACCATAGAGCGACGTTATTCTGATCTCACCA CCCTGGTGGCCTTTCCCTCCAGCTCTGTCTATCCTACCAAGGATGAGGAGAACAATCCCCTTGAGACAGA ATATGGCCTTTCTGTCTACAAGGATCACCAGACCATCACCATCCAGGAGATGCCGGAGAAGGCCCCAGCC GGCCAGCTCCCCCGCTCTGTGGACGTCATTCTGGATGATGACTTGGTGGATAAAGCGAAGCCTGGTGACC GGGTTCAGGTGGTGGGAACCTACCGTTGCCTTCCTGGAAAGAAGGGAGGCTACACCTCTGGGACCTTCAG GACTGTCCTGATTGCCTGTAATGTTAAGCAGATGAGCAAGGATGCTCAGCCCTCTTTCTCTGCTGAGGAT ATAGCCAAGATCAAGAAGTTCAGTAAAACCCGATCCAAGGATATCTTTGACCAGCTGGCCAAGTCATTGG CCCCAAGTATCCATGGGCATGACTATGTCAAGAAAGCAATCCTCTGCTTGCTCTTGGGAGGGGTGGAACG AGACCTAGAAAATGGCAGCCACATCCGTGGGGACATCAATATTCTTCTAATAGGAGACCCATCCGTTGCC AAGTCTCAGCTTCTGCGGTATGTGCTTTGCACTGCACCCCGAGCTATCCCCACCACTGGCCGGGGCTCCT CTGGAGTGGGTCTGACGGCTGCTGTCACCACAGACCAGGAAACAGGAGAGCGCCGTCTGGAAGCAGGGGC CATGGTCCTGGCTGACCGAGGCGTGGTTTGCATTGATGAATTTGACAAAATGTCTGACATGGATCGCACA GCCATCCATGAAGTGATGGAGCAGGGTCGAGTGACCATTGCCAAGGCTGGCATCCATGCTCGGCTGAATG CCCGCTGCAGTGTTTTGGCAGCTGCCAACCCTGTCTACGGCAGGTATGACCAGTATAAGACTCCAATGGA GAACATTGGGCTACAGGACTCACTGCTGTCACGATTTGACTTGCTCTTCATCATGCTGGATCAGATGGAT CCTGAGCAGGATCGGGAGATCTCAGACCATGTCCTTCGGATGCACCGTTACAGAGCACCTGGGGAGCAGG ATGGCGATGCTATGCCCTTGGGTAGTGCTGTGGATATCCTGGCCACAGATGATCCCAACTTTAGCCAGGA AGATCAGCAGGACACCCAGATTTATGAGAAGCATGACAACCTTCTACATGGGACCAAGAAGAAAAAGGAG AAGATGGTGAGTGCAGCATTCATGAAGAAGTACATCCATGTGGCCAAAATCATCAAGCCTGTCCTGACAC AGGAGTCGGCCACCTACATTGCAGAAGAGTATTCACGCCTGCGCAGCCAGGATAGCATGAGCTCAGACAC CGCCAGGACATCTCCAGTTACAGCCCGAACACTGGAAACTCTGATTCGACTGGCCACAGCCCATGCGAAG GCCCGCATGAGCAAGACTGTGGACCTGCAGGATGCAGAGGAAGCTGTGGAGTTGGTCCAGTATGCTTACT TTAAGAAGGTTCTGGAGAAGGAGAAGAAACGTAAGAAGCGAAGTGAGGATGAATCAGAGACAGAAGATGA AGAGGAGAAAAGCCAAGAGGACCAGGAGCAGAAGAGGAAGAGAAGGAAGACTCGCCAGCCAGATGCCAAA GATGGGGATTCATACGACCCCTATGACTTCAGTGACACAGAGGAGGAAATGCCTCAAGTACACACTCCAA AGACGGCAGACTCACAGGAGACCAAGGAATCCCAGAAAGTGGAGTTGAGTGAATCCAGGTTGAAGGCATT CAAGGTGGCCCTCTTGGATGTGTTCCGGGAAGCTCATGCGCAGTCAATCGGCATGAATCGCCTCACAGAA TCCATCAACCGGGACAGCGAAGAGCCCTTCTCTTCAGTTGAGATCCAGGCTGCTCTGAGCAAGATGCAGG ATGACAATCAGGTCATGGTGTCTGAGGGCATCATCTTCCTCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200208 protein sequence
Red=Cloning site Green=Tags(s) MAGTVVLDDVELREAQRDYLDFLDDEEDQGIYQSKVRELISDNQYRLIVNVNDLRRKNEKRANRLLNNAF EELVAFQRALKDFVASIDATYAKQYEEFYVGLEGSFGSKHVSPRTLTSCFLSCVVCVEGIVTKCSLVRPK VVRSVHYCPATKKTIERRYSDLTTLVAFPSSSVYPTKDEENNPLETEYGLSVYKDHQTITIQEMPEKAPA GQLPRSVDVILDDDLVDKAKPGDRVQVVGTYRCLPGKKGGYTSGTFRTVLIACNVKQMSKDAQPSFSAED IAKIKKFSKTRSKDIFDQLAKSLAPSIHGHDYVKKAILCLLLGGVERDLENGSHIRGDINILLIGDPSVA KSQLLRYVLCTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDMDRT AIHEVMEQGRVTIAKAGIHARLNARCSVLAAANPVYGRYDQYKTPMENIGLQDSLLSRFDLLFIMLDQMD PEQDREISDHVLRMHRYRAPGEQDGDAMPLGSAVDILATDDPNFSQEDQQDTQIYEKHDNLLHGTKKKKE KMVSAAFMKKYIHVAKIIKPVLTQESATYIAEEYSRLRSQDSMSSDTARTSPVTARTLETLIRLATAHAK ARMSKTVDLQDAEEAVELVQYAYFKKVLEKEKKRKKRSEDESETEDEEEKSQEDQEQKRKRRKTRQPDAK DGDSYDPYDFSDTEEEMPQVHTPKTADSQETKESQKVELSESRLKAFKVALLDVFREAHAQSIGMNRLTE SINRDSEEPFSSVEIQAALSKMQDDNQVMVSEGIIFLI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002388 |
| ORF Size | 2424 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002388.6 |
| RefSeq Size | 3234 bp |
| RefSeq ORF | 2427 bp |
| Locus ID | 4172 |
| UniProt ID | P25205 |
| Domains | MCM, AAA |
| Protein Families | Druggable Genome, Stem cell - Pluripotency, Transcription Factors |
| Protein Pathways | Cell cycle, DNA replication |
| MW | 91 kDa |
| Gene Summary | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2018] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Minichromosome maintenance proteins 2, 3 and 7 in medulloblastoma: overexpression and involvement in regulation of cell migration and invasion
,K-M Lau, Q K Y Chan, J C S Pang, K K W Li, W W Yeung, N Y F Chung, P C Lui, Y-S Tam, H-M Li, L Zhou, et al.,
Oncogene (26 July 2010) doi:10.1038/onc.2010.287 Original Article
[MCM3]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200208L3 | Lenti ORF clone of Human minichromosome maintenance complex component 3 (MCM3), Myc-DDK-tagged |
CNY 8,840.00 |
|
| RC200208L4 | Lenti ORF clone of Human minichromosome maintenance complex component 3 (MCM3), mGFP tagged |
CNY 8,840.00 |
|
| RG200208 | MCM3 (tGFP-tagged) - Human minichromosome maintenance complex component 3 (MCM3) |
CNY 7,600.00 |
|
| SC118658 | MCM3 (untagged)-Human minichromosome maintenance complex component 3 (MCM3) |
CNY 8,888.00 |
