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PLCG 2 (PLCG2) (NM_002661) Human Tagged ORF Clone
CAT＃: RC200442
PLCG2 (Myc-DDK-tagged)-Human phospholipase C, gamma 2 (phosphatidylinositol-specific) (PLCG2)
ORF Plasmid: tGFP
"NM_002661" in other vectors (7)
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CNY 7,600.00
| Cited in 3 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | APLAID; FCAS3; PLC-gamma-2; PLC-IV |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200442 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCACCACGGTCAATGTAGATTCCCTTGCGGAATATGAGAAGAGCCAGATCAAGAGAGCCCTGGAGC TGGGGACGGTGATGACTGTGTTCAGCTTCCGCAAGTCCACCCCCGAGCGGAGAACCGTCCAGGTGATCAT GGAGACGCGGCAGGTGGCCTGGAGCAAGACCGCCGACAAGATCGAGGGCTTCTTGGATATCATGGAAATA AAAGAAATCCGCCCAGGGAAGAACTCCAAAGATTTCGAGCGAGCAAAAGCAGTTCGCCAGAAAGAAGACT GCTGCTTCACCATCCTATATGGCACTCAGTTCGTCCTCAGCACGCTCAGCTTGGCAGCTGACTCTAAAGA GGATGCAGTTAACTGGCTCTCTGGCTTGAAAATCTTACACCAGGAAGCGATGAATGCGTCCACGCCCACC ATTATCGAGAGTTGGCTGAGAAAGCAGATATATTCTGTGGATCAAACCAGAAGAAACAGCATCAGTCTCC GAGAGTTGAAGACCATCTTGCCCCTGATCAACTTTAAAGTGAGCAGTGCCAAGTTCCTTAAAGATAAGTT TGTGGAAATAGGAGCACACAAAGATGAGCTCAGCTTTGAACAGTTCCATCTCTTCTATAAAAAACTTATG TTTGAACAGCAAAAATCGATTCTCGATGAATTCAAAAAGGATTCGTCCGTGTTCATCCTGGGGAACACTG ACAGGCCGGATGCCTCTGCTGTTTACCTGCGTGACTTCCAGAGGTTTCTCATACATGAACAGCAGGAGCA TTGGGCTCAGGATCTGAACAAAGTCCGTGAGCGGATGACAAAGTTCATTGATGACACCATGCGTGAAACT GCTGAGCCTTTCTTGTTTGTGGATGAGTTCCTCACGTACCTGTTTTCACGAGAAAACAGCATCTGGGATG AGAAGTATGACGCGGTGGACATGCAGGACATGAACAACCCCCTGTCTCATTACTGGATCTCCTCGTCACA TAACACGTACCTTACAGGTGACCAGCTGCGGAGCGAGTCGTCCCCAGAAGCTTACATCCGCTGCCTGCGC ATGGGCTGTCGCTGCATTGAACTGGACTGCTGGGACGGGCCCGATGGGAAGCCGGTCATCTACCATGGCT GGACGCGGACTACCAAGATCAAGTTTGACGACGTCGTGCAGGCCATCAAAGACCACGCCTTTGTTACGTC GAGCTTCCCAGTGATCCTGTCCATCGAGGAGCACTGCAGCGTGGAGCAACAGCGTCACATGGCCAAGGCC TTCAAGGAAGTATTTGGCGACCTGCTGTTGACGAAGCCCACGGAGGCCAGTGCTGACCAGCTGCCCTCGC CCAGCCAGCTGCGGGAGAAGATCATCATCAAGCATAAGAAGCTGGGCCCCCGAGGCGATGTGGATGTCAA CATGGAGGACAAGAAGGACGAACACAAGCAACAGGGGGAGCTGTACATGTGGGATTCCATTGACCAGAAA TGGACTCGGCACTACTGCGCCATTGCCGATGCCAAGCTGTCCTTCAGTGATGACATTGAACAGACTATGG AGGAGGAAGTGCCCCAGGATATACCCCCTACAGAACTACATTTTGGGGAGAAATGGTTCCACAAGAAGGT GGAGAAGAGGACGAGTGCCGAGAAGTTGCTGCAGGAATACTGCATGGAGACGGGGGGCAAGGATGGCACC TTCCTGGTTCGGGAGAGCGAGACCTTCCCCAATGACTACACCCTGTCCTTCTGGCGGTCAGGCCGGGTCC AGCACTGCCGGATCCGCTCCACCATGGAGGGCGGGACCCTGAAATACTACTTGACTGACAACCTCACCTT CAGCAGCATCTATGCCCTCATCCAGCACTACCGCGAGACGCACCTGCGCTGCGCCGAGTTCGAGCTGCGG CTCACGGACCCTGTGCCCAACCCCAACCCCCACGAGTCCAAGCCGTGGTACTATGACAGCCTGAGCCGCG GAGAGGCAGAGGACATGCTGATGAGGATTCCCCGGGACGGGGCCTTCCTGATCCGGAAGCGAGAGGGGAG CGACTCCTATGCCATCACCTTCAGGGCTAGGGGCAAGGTAAAGCATTGTCGCATCAACCGGGACGGCCGG CACTTTGTGCTGGGGACCTCCGCCTATTTTGAGAGTCTGGTGGAGCTCGTCAGTTACTACGAGAAGCATT CACTCTACCGAAAGATGAGACTGCGCTACCCCGTGACCCCCGAGCTCCTGGAGCGCTACAATATGGAAAG AGATATAAACTCCCTCTACGACGTCAGCAGAATGTATGTGGATCCCAGTGAAATCAATCCGTCCATGCCT CAGAGAACCGTGAAAGCTCTGTATGACTACAAAGCCAAGCGAAGCGATGAGCTGAGCTTCTGCCGTGGTG CCCTCATCCACAATGTCTCCAAGGAGCCCGGGGGCTGGTGGAAAGGAGACTATGGAACCAGGATCCAGCA GTACTTCCCATCCAACTACGTCGAGGACATCTCAACTGCAGACTTCGAGGAGCTAGAAAAGCAGATTATT GAAGACAATCCCTTAGGGTCTCTTTGCAGAGGAATATTGGACCTCAATACCTATAACGTCGTGAAAGCCC CTCAGGGAAAAAACCAGAAGTCCTTTGTCTTCATCCTGGAGCCCAAGCAGCAGGGCTATCCTCCGGTGGA GTTTGCCACAGACAGGGTGGAGGAGCTCTTTGAGTGGTTTCAGAGCATCCGAGAGATCACCTGGAAGATT GACACCAAGGAGAACAACATGAAGTACTGGGAGAAGAACCAGTCCATCGCCATCGAGCTCTCTGACCTGG TTGTCTACTGCAAACCAACCAGCAAAACCAAGGACAACTTAGAAAATCCTGACTTCCGAGAAATCCGCTC CTTTGTGGAGACGAAGGCTGACAGCATCATCAGACAGAAGCCCGTCGACCTCCTGAAGTACAATCAAAAG GGCCTGACCCGCGTCTACCCAAAGGGACAAAGAGTTGACTCTTCAAACTACGACCCCTTCCGCCTCTGGC TGTGCGGTTCTCAGATGGTGGCACTCAATTTCCAGACGGCAGATAAGTACATGCAGATGAATCACGCATT GTTTTCTCTCAACGGGCGCACGGGCTACGTTCTGCAGCCTGAGAGCATGAGGACAGAGAAATATGACCCG ATGCCACCCGAGTCCCAGAGGAAGATCCTGATGACGCTGACAGTCAAGGTTCTCGGTGCTCGCCATCTCC CCAAACTTGGACGAAGTATTGCCTGTCCCTTTGTAGAAGTGGAGATCTGTGGAGCCGAGTATGACAACAA CAAGTTCAAGACGACGGTTGTGAATGATAATGGCCTCAGCCCTATCTGGGCTCCAACACAGGAGAAGGTG ACATTTGAAATTTATGACCCAAACCTGGCATTTCTGCGCTTTGTGGTTTATGAAGAAGATATGTTCAGCG ATCCCAACTTTCTTGCTCATGCCACTTACCCCATTAAAGCAGTCAAATCAGGATTCAGGTCCGTTCCTCT GAAGAATGGGTACAGCGAGGACATAGAGCTGGCTTCCCTCCTGGTTTTCTGTGAGATGCGGCCAGTCCTG GAGAGCGAAGAGGAACTTTACTCCTCCTGTCGCCAGCTGAGGAGGCGGCAAGAAGAACTGAACAACCAGC TCTTTCTGTATGACACACACCAGAACTTGCGCAATGCCAACCGGGATGCCCTGGTTAAAGAGTTCAGTGT TAATGAGAACCAGCTCCAGCTGTACCAGGAGAAATGCAACAAGAGGTTAAGAGAGAAGAGAGTCAGCAAC AGCAAGTTTTACTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200442 protein sequence
Red=Cloning site Green=Tags(s) MSTTVNVDSLAEYEKSQIKRALELGTVMTVFSFRKSTPERRTVQVIMETRQVAWSKTADKIEGFLDIMEI KEIRPGKNSKDFERAKAVRQKEDCCFTILYGTQFVLSTLSLAADSKEDAVNWLSGLKILHQEAMNASTPT IIESWLRKQIYSVDQTRRNSISLRELKTILPLINFKVSSAKFLKDKFVEIGAHKDELSFEQFHLFYKKLM FEQQKSILDEFKKDSSVFILGNTDRPDASAVYLRDFQRFLIHEQQEHWAQDLNKVRERMTKFIDDTMRET AEPFLFVDEFLTYLFSRENSIWDEKYDAVDMQDMNNPLSHYWISSSHNTYLTGDQLRSESSPEAYIRCLR MGCRCIELDCWDGPDGKPVIYHGWTRTTKIKFDDVVQAIKDHAFVTSSFPVILSIEEHCSVEQQRHMAKA FKEVFGDLLLTKPTEASADQLPSPSQLREKIIIKHKKLGPRGDVDVNMEDKKDEHKQQGELYMWDSIDQK WTRHYCAIADAKLSFSDDIEQTMEEEVPQDIPPTELHFGEKWFHKKVEKRTSAEKLLQEYCMETGGKDGT FLVRESETFPNDYTLSFWRSGRVQHCRIRSTMEGGTLKYYLTDNLTFSSIYALIQHYRETHLRCAEFELR LTDPVPNPNPHESKPWYYDSLSRGEAEDMLMRIPRDGAFLIRKREGSDSYAITFRARGKVKHCRINRDGR HFVLGTSAYFESLVELVSYYEKHSLYRKMRLRYPVTPELLERYNMERDINSLYDVSRMYVDPSEINPSMP QRTVKALYDYKAKRSDELSFCRGALIHNVSKEPGGWWKGDYGTRIQQYFPSNYVEDISTADFEELEKQII EDNPLGSLCRGILDLNTYNVVKAPQGKNQKSFVFILEPKQQGYPPVEFATDRVEELFEWFQSIREITWKI DTKENNMKYWEKNQSIAIELSDLVVYCKPTSKTKDNLENPDFREIRSFVETKADSIIRQKPVDLLKYNQK GLTRVYPKGQRVDSSNYDPFRLWLCGSQMVALNFQTADKYMQMNHALFSLNGRTGYVLQPESMRTEKYDP MPPESQRKILMTLTVKVLGARHLPKLGRSIACPFVEVEICGAEYDNNKFKTTVVNDNGLSPIWAPTQEKV TFEIYDPNLAFLRFVVYEEDMFSDPNFLAHATYPIKAVKSGFRSVPLKNGYSEDIELASLLVFCEMRPVL ESEEELYSSCRQLRRRQEELNNQLFLYDTHQNLRNANRDALVKEFSVNENQLQLYQEKCNKRLREKRVSN SKFYS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002661 |
| ORF Size | 3795 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002661.5 |
| RefSeq Size | 8707 bp |
| RefSeq ORF | 3798 bp |
| Locus ID | 5336 |
| UniProt ID | P16885 |
| Domains | C2, PI-PLC-X, SH2, SH3, PI-PLC-Y, PH |
| Protein Families | Druggable Genome |
| Protein Pathways | B cell receptor signaling pathway, Calcium signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Glioma, Inositol phosphate metabolism, Leukocyte transendothelial migration, Metabolic pathways, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Phosphatidylinositol signaling system, VEGF signaling pathway, Vibrio cholerae infection |
| MW | 147.9 kDa |
| Gene Summary | The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. [provided by RefSeq, Mar 2014] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Heterozygous expression of the Alzheimer’s disease-protective PLCγ2 P522R variant enhances Aβ clearance while preserving synapses
,null,
Cellular and Molecular Life Sciences
,PubMed ID 35895133
[PLCG 2]
|
|
A Germline Mutation in the C2 Domain of PLCγ2 Associated with Gain-of-Function Expands the Phenotype for PLCG2-Related Diseases
,Novice, T;Kariminia, A;Del Bel, KL;Lu, H;Sharma, M;Lim, CJ;Read, J;Lugt, MV;Hannibal, MC;O'Dwyer, D;Hosler, M;Scharnitz, T;Rizzo, JM;Zacur, J;Priatel, J;Abdossamadi, S;Bohm, A;Junker, A;Turvey, SE;Schultz, KR;Rozmus, J;,
J. Clin. Immunol.
,PubMed ID 31853824
[PLCG 2]
|
|
Alzheimer's disease phospholipase C-gamma-2 (PLCG2) protective variant is a functional hypermorph
,Magno, L;Lessard, CB;Martins, M;Lang, V;Cruz, P;Asi, Y;Katan, M;Bilsland, J;Lashley, T;Chakrabarty, P;Golde, TE;Whiting, PJ;,
Alzheimers Res Ther
,PubMed ID 30711010
[PLCG 2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200442L1 | Lenti ORF clone of Human phospholipase C, gamma 2 (phosphatidylinositol-specific) (PLCG2), Myc-DDK-tagged |
CNY 15,608.00 |
|
| RC200442L2 | Lenti ORF clone of Human phospholipase C, gamma 2 (phosphatidylinositol-specific) (PLCG2), mGFP tagged |
CNY 15,608.00 |
|
| RC200442L3 | Lenti ORF clone of Human phospholipase C, gamma 2 (phosphatidylinositol-specific) (PLCG2), Myc-DDK-tagged |
CNY 15,608.00 |
|
| RC200442L4 | Lenti ORF clone of Human phospholipase C, gamma 2 (phosphatidylinositol-specific) (PLCG2), mGFP tagged |
CNY 15,608.00 |
|
| RG200442 | PLCG2 (tGFP-tagged) - Human phospholipase C, gamma 2 (phosphatidylinositol-specific) (PLCG2) |
CNY 14,808.00 |
|
| SC118495 | PLCG2 (untagged)-Human phospholipase C, gamma 2 (phosphatidylinositol-specific) (PLCG2) |
CNY 13,088.00 |
|
| SC318575 | PLCG2 (untagged)-Human phospholipase C, gamma 2 (phosphatidylinositol-specific) (PLCG2) |
CNY 14,104.00 |
