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MATK (NM_139354) Human Tagged ORF Clone
CAT＃: RC200454
MATK (Myc-DDK-tagged)-Human megakaryocyte-associated tyrosine kinase (MATK), transcript variant 3
ORF Plasmid: tGFP
"NM_139354" in other vectors (6)
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CNY 3,776.00
CNY 5,320.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CHK; CTK; HHYLTK; HYL; HYLTK; Lsk |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200454 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGGCGAGGCTCTCTGGTTTCCTGGCGGGCATTTCACGGCTGTGATTCTGCTGAGGAACTTCCCC GGGTGAGCCCCCGCTTCCTCCGAGCCTGGCACCCCCCTCCCGTCTCAGCCAGGATGCCAACGAGGCGCTG GGCCCCGGGCACCCAGTGTATCACCAAATGCGAGCACACCCGCCCCAAGCCAGGGGAGCTGGCCTTCCGC AAGGGCGACGTGGTCACCATCCTGGAGGCCTGCGAGAACAAGAGCTGGTACCGCGTCAAGCACCACACCA GTGGACAGGAGGGGCTGCTGGCAGCTGGGGCGCTGCGGGAGCGGGAGGCCCTCTCCGCAGACCCCAAGCT CAGCCTCATGCCGTGGTTCCACGGGAAGATCTCGGGCCAGGAGGCTGTCCAGCAGCTGCAGCCTCCCGAG GATGGGCTGTTCCTGGTGCGGGAGTCCGCGCGCCACCCCGGCGACTACGTCCTGTGCGTGAGCTTTGGCC GCGACGTCATCCACTACCGCGTGCTGCACCGCGACGGCCACCTCACAATCGATGAGGCCGTGTTCTTCTG CAACCTCATGGACATGGTGGAGCATTACAGCAAGGACAAGGGCGCTATCTGCACCAAGCTGGTGAGACCA AAGCGGAAACACGGGACCAAGTCGGCCGAGGAGGAGCTGGCCAGGGCGGGCTGGTTACTGAACCTGCAGC ATTTGACATTGGGAGCACAGATCGGAGAGGGAGAGTTTGGAGCTGTCCTGCAGGGTGAGTACCTGGGGCA AAAGGTGGCCGTGAAGAATATCAAGTGTGATGTGACAGCCCAGGCCTTCCTGGACGAGACGGCCGTCATG ACGAAGATGCAACACGAGAACCTGGTGCGTCTCCTGGGCGTGATCCTGCACCAGGGGCTGTACATTGTCA TGGAGCACGTGAGCAAGGGCAACCTGGTGAACTTTCTGCGGACCCGGGGTCGAGCCCTCGTGAACACCGC TCAGCTCCTGCAGTTTTCTCTGCACGTGGCCGAGGGCATGGAGTACCTGGAGAGCAAGAAGCTTGTGCAC CGCGACCTGGCCGCCCGCAACATCCTGGTCTCAGAGGACCTGGTGGCCAAGGTCAGCGACTTTGGCCTGG CCAAAGCCGAGCGGAAGGGGCTAGACTCAAGCCGGCTGCCCGTCAAGTGGACGGCGCCCGAGGCTCTCAA ACACGGGAAGTTCACCAGCAAGTCGGATGTCTGGAGTTTTGGGGTGCTGCTCTGGGAGGTCTTCTCATAT GGACGGGCTCCGTACCCTAAAATGTCACTGAAAGAGGTGTCGGAGGCCGTGGAGAAGGGGTACCGCATGG AACCCCCCGAGGGCTGTCCAGGCCCCGTGCACGTCCTCATGAGCAGCTGCTGGGAGGCAGAGCCCGCCCG CCGGCCACCCTTCCGCAAACTGGCCGAGAAGCTGGCCCGGGAGCTACGCAGTGCAGGTGCCCCAGCCTCC GTCTCAGGGCAGGACGCCGACGGCTCCACCTCGCCCCGAAGCCAGGAGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200454 protein sequence
Red=Cloning site Green=Tags(s) MAGRGSLVSWRAFHGCDSAEELPRVSPRFLRAWHPPPVSARMPTRRWAPGTQCITKCEHTRPKPGELAFR KGDVVTILEACENKSWYRVKHHTSGQEGLLAAGALREREALSADPKLSLMPWFHGKISGQEAVQQLQPPE DGLFLVRESARHPGDYVLCVSFGRDVIHYRVLHRDGHLTIDEAVFFCNLMDMVEHYSKDKGAICTKLVRP KRKHGTKSAEEELARAGWLLNLQHLTLGAQIGEGEFGAVLQGEYLGQKVAVKNIKCDVTAQAFLDETAVM TKMQHENLVRLLGVILHQGLYIVMEHVSKGNLVNFLRTRGRALVNTAQLLQFSLHVAEGMEYLESKKLVH RDLAARNILVSEDLVAKVSDFGLAKAERKGLDSSRLPVKWTAPEALKHGKFTSKSDVWSFGVLLWEVFSY GRAPYPKMSLKEVSEAVEKGYRMEPPEGCPGPVHVLMSSCWEAEPARRPPFRKLAEKLARELRSAGAPAS VSGQDADGSTSPRSQEP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_139354 |
| ORF Size | 1521 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 1940 bp |
| RefSeq ORF | 1401 bp |
| Locus ID | 4145 |
| UniProt ID | P42679 |
| Protein Families | Druggable Genome, Protein Kinase, Stem cell - Pluripotency |
| MW | 56.5 kDa |
| Gene Summary | The protein encoded by this gene has amino acid sequence similarity to Csk tyrosine kinase and has the structural features of the CSK subfamily: SRC homology SH2 and SH3 domains, a catalytic domain, a unique N terminus, lack of myristylation signals, lack of a negative regulatory phosphorylation site, and lack of an autophosphorylation site. This protein is thought to play a significant role in the signal transduction of hematopoietic cells. It is able to phosphorylate and inactivate Src family kinases, and may play an inhibitory role in the control of T-cell proliferation. This protein might be involved in signaling in some cases of breast cancer. Three alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200454L1 | Lenti ORF clone of Human megakaryocyte-associated tyrosine kinase (MATK), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200454L2 | Lenti ORF clone of Human megakaryocyte-associated tyrosine kinase (MATK), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RC200454L3 | Lenti ORF clone of Human megakaryocyte-associated tyrosine kinase (MATK), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200454L4 | Lenti ORF clone of Human megakaryocyte-associated tyrosine kinase (MATK), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RG200454 | MATK (tGFP-tagged) - Human megakaryocyte-associated tyrosine kinase (MATK), transcript variant 3 |
CNY 4,370.00 |
|
| SC321587 | MATK (untagged)-Human megakaryocyte-associated tyrosine kinase (MATK), transcript variant 3 |
CNY 3,656.00 |
