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OTUD5 (NM_017602) Human Tagged ORF Clone
CAT＃: RC200893
OTUD5 (Myc-DDK-tagged)-Human OTU domain containing 5 (OTUD5), transcript variant 1
ORF Plasmid: tGFP
"NM_017602" in other vectors (6)
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CNY 6,376.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DUBA; MCAND |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200893 representing NM_017602
Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGACTATACTCCCCAAAAAGAAGCCGCCGCCTCCCGACGCCGACCCCGCCAACGAGCCGCCGCCGCCCG GGCCGATGCCCCCGGCGCCGCGGCGCGGCGGAGGTGTGGGCGTGGGCGGCGGCGGCACGGGCGTGGGCGG CGGCGATCGCGACCGTGACTCCGGCGTCGTGGGGGCCCGTCCGCGAGCTTCGCCACCGCCTCAAGGCCCG CTACCAGGACCGCCGGGCGCTCTTCATCGCTGGGCGCTGGCCGTGCCGCCTGGTGCAGTGGCGGGTCCCC GGCCACAACAGGCTTCTCCACCTCCTTGCGGGGGCCCAGGTGGTCCCGGCGGCGGTCCCGGCGACGCGCT GGGCGCAGCGGCGGCGGGTGTGGGTGCCGCGGGCGTGGTGGTGGGTGTGGGTGGTGCCGTAGGCGTGGGC GGCTGCTGCTCCGGGCCTGGGCACAGCAAGCGGCGACGTCAAGCTCCCGGGGTTGGCGCGGTTGGCGGGG GCAGTCCCGAGCGTGAGGAGGTCGGCGCAGGCTACAACAGTGAGGACGAGTATGAGGCGGCTGCAGCACG CATCGAGGCTATGGACCCTGCCACTGTCGAGCAGCAGGAGCATTGGTTTGAAAAGGCCCTACGAGACAAG AAGGGCTTCATCATCAAGCAGATGAAGGAGGATGGCGCCTGTCTCTTCCGGGCTGTAGCTGACCAGGTGT ATGGAGACCAGGACATGCATGAGGTTGTGCGAAAGCATTGCATGGACTATCTGATGAAGAATGCCGACTA CTTCTCCAACTATGTCACAGAGGACTTTACCACCTACATTAACAGGAAGCGGAAAAACAATTGCCATGGC AACCACATTGAGATGCAGGCCATGGCAGAGATGTACAACCGTCCTGTGGAGGTGTACCAGTACAGCACAG GTACTTCTGCAGTGGAACCCATCAACACATTCCATGGGATACATCAAAACGAGGACGAACCCATTCGTGT TAGCTACCATCGGAATATCCACTATAATTCAGTGGTGAATCCTAACAAGGCCACCATTGGTGTGGGGCTG GGCCTGCCATCATTCAAACCAGGGTTTGCAGAGCAGTCTCTGATGAAGAATGCCATAAAAACATCGGAGG AGTCATGGATTGAACAGCAGATGCTAGAAGACAAGAAACGGGCCACAGACTGGGAGGCCACAAATGAAGC CATCGAGGAGCAGGTGGCTCGGGAATCCTACCTGCAGTGGTTGCGGGATCAGGAGAAACAGGCTCGCCAG GTCCGAGGCCCCAGCCAGCCCCGGAAAGCCAGCGCCACATGCAGTTCGGCCACAGCAGCAGCCTCCAGTG GCCTGGAGGAGTGGACTAGCCGGTCCCCGCGGCAGCGGAGTTCAGCCTCGTCACCTGAGCACCCTGAGCT GCATGCTGAATTGGGCATGAAGCCCCCTTCCCCAGGCACTGTTTTAGCTCTTGCCAAACCTCCTTCGCCC TGTGCGCCAGGTACAAGCAGTCAGTTCTCGGCAGGGGCCGACCGGGCAACTTCCCCCCTTGTGTCCCTCT ACCCTGCTTTGGAGTGCCGGGCCCTCATTCAGCAGATGTCCCCCTCTGCCTTTGGTCTGAATGACTGGGA TGATGATGAGATCCTAGCTTCGGTGCTGGCAGTGTCCCAACAGGAATACCTAGACAGTATGAAGAAAAAC AAAGTGCACAGAGACCCGCCCCCAGACAAGAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200893 representing NM_017602
Red=Cloning site Green=Tags(s) MTILPKKKPPPPDADPANEPPPPGPMPPAPRRGGGVGVGGGGTGVGGGDRDRDSGVVGARPRASPPPQGP LPGPPGALHRWALAVPPGAVAGPRPQQASPPPCGGPGGPGGGPGDALGAAAAGVGAAGVVVGVGGAVGVG GCCSGPGHSKRRRQAPGVGAVGGGSPEREEVGAGYNSEDEYEAAAARIEAMDPATVEQQEHWFEKALRDK KGFIIKQMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHG NHIEMQAMAEMYNRPVEVYQYSTGTSAVEPINTFHGIHQNEDEPIRVSYHRNIHYNSVVNPNKATIGVGL GLPSFKPGFAEQSLMKNAIKTSEESWIEQQMLEDKKRATDWEATNEAIEEQVARESYLQWLRDQEKQARQ VRGPSQPRKASATCSSATAAASSGLEEWTSRSPRQRSSASSPEHPELHAELGMKPPSPGTVLALAKPPSP CAPGTSSQFSAGADRATSPLVSLYPALECRALIQQMSPSAFGLNDWDDDEILASVLAVSQQEYLDSMKKN KVHRDPPPDKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
AscI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017602 |
| ORF Size | 1713 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017602.4 |
| RefSeq Size | 2759 bp |
| RefSeq ORF | 1716 bp |
| Locus ID | 55593 |
| UniProt ID | Q96G74 |
| Protein Families | Protease |
| Protein Pathways | RIG-I-like receptor signaling pathway |
| MW | 60.4 kDa |
| Gene Summary | This gene encodes a member of the OTU (ovarian tumor) domain-containing cysteine protease superfamily. The OTU domain confers deubiquitinase activity and the encoded protein has been shown to suppress the type I interferon-dependent innate immune response by cleaving the polyubiquitin chain from an essential type I interferon adaptor protein. Cleavage results in disassociation of the adaptor protein from a downstream signaling complex and disruption of the type I interferon signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Oct 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200893L1 | Lenti-ORF clone of OTUD5 (Myc-DDK-tagged)-Human OTU domain containing 5 (OTUD5), transcript variant 1 |
CNY 8,776.00 |
|
| RC200893L2 | Lenti-ORF clone of OTUD5 (mGFP-tagged)-Human OTU domain containing 5 (OTUD5), transcript variant 1 |
CNY 6,460.00 |
|
| RC200893L3 | Lenti-ORF clone of OTUD5 (Myc-DDK-tagged)-Human OTU domain containing 5 (OTUD5), transcript variant 1 |
CNY 6,460.00 |
|
| RC200893L4 | Lenti-ORF clone of OTUD5 (mGFP-tagged)-Human OTU domain containing 5 (OTUD5), transcript variant 1 |
CNY 6,460.00 |
|
| RG200893 | OTUD5 (tGFP-tagged) - Human OTU domain containing 5 (OTUD5), transcript variant 1 |
CNY 5,040.00 |
|
| SC107540 | OTUD5 (untagged)-Human OTU domain containing 5 (OTUD5), transcript variant 1 |
CNY 6,384.00 |
