KLF6 (NM_001300) Human Tagged ORF Clone
CAT#: RC201763
KLF6 (Myc-DDK-tagged)-Human Kruppel-like factor 6 (KLF6), transcript variant A
ORF Plasmid: tGFP
"NM_001300" in other vectors (6)
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CNY 3,600.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | BCD1; CBA1; COPEB; CPBP; GBF; PAC1; ST12; ZF9 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC201763 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGTGCTCCCCATGTGCAGCATCTTCCAGGAGCTCCAGATCGTGCACGAGACCGGCTACTTCTCGG CGCTGCCGTCTCTGGAGGAGTACTGGCAACAGACCTGCCTAGAGCTGGAACGTTACCTCCAGAGCGAGCC CTGCTATGTTTCAGCCTCAGAAATCAAATTTGACAGCCAGGAAGATCTGTGGACCAAAATCATTCTGGCT CGGGAGAAAAAGGAGGAATCCGAACTGAAGATATCTTCCAGTCCTCCAGAGGACACTCTCATCAGCCCGA GCTTTTGTTACAACTTAGAGACCAACAGCCTGAACTCAGATGTCAGCAGCGAATCCTCTGACAGCTCCGA GGAACTTTCTCCCACGGCCAAGTTTACCTCCGACCCCATTGGCGAAGTTTTGGTCAGCTCGGGAAAATTG AGCTCCTCTGTCACCTCCACGCCTCCATCTTCTCCGGAACTGAGCAGGGAACCTTCTCAACTGTGGGGTT GCGTGCCCGGGGAGCTGCCCTCGCCAGGGAAGGTGCGCAGCGGGACTTCGGGGAAGCCAGGTGACAAGGG AAATGGCGATGCCTCCCCCGACGGCAGGAGGAGGGTGCACCGGTGCCACTTTAACGGCTGCAGGAAAGTT TACACCAAAAGCTCCCACTTGAAAGCACACCAGCGGACGCACACAGGAGAAAAGCCTTACAGATGCTCAT GGGAAGGGTGTGAGTGGCGTTTTGCAAGAAGTGATGAGTTAACCAGGCACTTCCGAAAGCACACCGGGGC CAAGCCTTTTAAATGCTCCCACTGTGACAGGTGTTTTTCCAGGTCTGACCACCTGGCCCTGCACATGAAG AGGCACCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201763 protein sequence
Red=Cloning site Green=Tags(s) MDVLPMCSIFQELQIVHETGYFSALPSLEEYWQQTCLELERYLQSEPCYVSASEIKFDSQEDLWTKIILA REKKEESELKISSSPPEDTLISPSFCYNLETNSLNSDVSSESSDSSEELSPTAKFTSDPIGEVLVSSGKL SSSVTSTPPSSPELSREPSQLWGCVPGELPSPGKVRSGTSGKPGDKGNGDASPDGRRRVHRCHFNGCRKV YTKSSHLKAHQRTHTGEKPYRCSWEGCEWRFARSDELTRHFRKHTGAKPFKCSHCDRCFSRSDHLALHMK RHL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001300 |
ORF Size | 849 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001300.6 |
RefSeq Size | 4679 bp |
RefSeq ORF | 852 bp |
Locus ID | 1316 |
UniProt ID | Q99612 |
Domains | zf-C2H2 |
Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors |
MW | 31.9 kDa |
Gene Summary | This gene encodes a member of the Kruppel-like family of transcription factors. The zinc finger protein is a transcriptional activator, and functions as a tumor suppressor. Multiple transcript variants encoding different isoforms have been found for this gene, some of which are implicated in carcinogenesis. [provided by RefSeq, May 2009] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
Expression Signatures of the Lipid-Based Akt Inhibitors Phosphatidylinositol Ether Lipid Analogues in NSCLC Cells
,Chunyu Zhang, Abdel G. Elkahloun, Hongling Liao, Shannon Delaney, Barbara Saber, Betsy Morrow, George C. Prendergast, M. Christine Hollander, Joell J. Gills, and Phillip A. Dennis,
Mol. Cancer Ther., Jul 2011; 10: 1137 - 1148.
[KLF6]
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201763L1 | Lenti ORF clone of Human Kruppel-like factor 6 (KLF6), transcript variant A, Myc-DDK-tagged |
CNY 6,000.00 |
|
RC201763L2 | Lenti ORF clone of Human Kruppel-like factor 6 (KLF6), transcript variant A, mGFP tagged |
CNY 6,000.00 |
|
RC201763L3 | Lenti ORF clone of Human Kruppel-like factor 6 (KLF6), transcript variant A, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC201763L4 | Lenti ORF clone of Human Kruppel-like factor 6 (KLF6), transcript variant A, mGFP tagged |
CNY 5,890.00 |
|
RG201763 | KLF6 (tGFP-tagged) - Human Kruppel-like factor 6 (KLF6), transcript variant A |
CNY 5,200.00 |
|
SC119291 | KLF6 (untagged)-Human Kruppel-like factor 6 (KLF6), transcript variant A |
CNY 3,600.00 |