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TRIM21 (NM_003141) Human Tagged ORF Clone
CAT＃: RC202088
TRIM21 (Myc-DDK-tagged)-Human tripartite motif containing 21 (TRIM21)
ORF Plasmid: tGFP
"NM_003141" in other vectors (6)
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CNY 5,488.00
| Cited in 2 publications. |
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RNF81; Ro/SSA; RO52; SSA; SSA1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202088 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCAGCAGCACGCTTGACAATGATGTGGGAGGAGGTCACATGCCCTATCTGCCTGGACCCCTTCG TGGAGCCTGTGAGCATCGAGTGTGGCCACAGCTTCTGCCAGGAATGCATCTCTCAGGTTGGGAAAGGTGG GGGCAGCGTCTGTCCTGTGTGCCGGCAGCGCTTTCTGCTCAAGAATCTCCGGCCCAATCGACAGCTAGCC AACATGGTGAACAACCTTAAAGAAATCAGCCAGGAGGCCAGAGAGGGCACACAGGGGGAACGGTGTGCAG TGCATGGAGAGAGACTTCACCTGTTCTGTGAGAAAGATGGGAAGGCCCTTTGCTGGGTATGTGCCCAGTC TCGGAAACACCGTGACCACGCCATGGTCCCTCTTGAGGAGGCTGCACAGGAGTACCAGGAGAAGCTCCAG GTGGCATTAGGGGAACTGAGAAGAAAGCAGGAGTTGGCTGAGAAGTTGGAAGTGGAAATTGCAATAAAGA GAGCAGACTGGAAGAAAACAGTGGAAACACAGAAATCTAGGATTCACGCAGAGTTTGTGCAGCAAAAAAA CTTCCTGGTTGAAGAAGAACAGAGGCAGCTGCAGGAGCTGGAGAAGGATGAGAGGGAGCAGCTGAGAATC CTGGGGGAGAAAGAGGCCAAGCTGGCCCAGCAGAGCCAGGCCCTACAGGAGCTCATCTCAGAGCTAGATC GAAGGTGCCACAGCTCAGCACTGGAACTGCTGCAGGAGGTGATAATTGTCCTGGAAAGGAGTGAGTCCTG GAACCTGAAGGACCTGGATATTACCTCTCCAGAACTCAGGAGTGTGTGCCATGTGCCAGGGCTGAAGAAG ATGCTGAGGACATGTGCAGTCCACATCACTCTGGATCCAGACACAGCCAATCCGTGGCTGATACTTTCAG AAGATCGGAGACAAGTGAGGCTTGGAGACACCCAGCAGAGCATACCTGGAAATGAAGAGAGATTTGATAG TTATCCTATGGTCCTGGGTGCCCAGCACTTTCACTCTGGAAAACATTACTGGGAGGTAGATGTGACAGGA AAGGAGGCCTGGGACCTGGGTGTCTGCAGAGACTCTGTGCGCAGGAAGGGGCACTTTTTGCTTAGTTCCA AGAGTGGCTTCTGGACAATTTGGTTGTGGAACAAACAAAAATATGAGGCTGGCACCTACCCCCAGACTCC CCTCCACCTTCAGGTGCCTCCATGCCAAGTTGGGATTTTCCTGGACTATGAGGCTGGCATGGTCTCCTTC TACAACATCACTGACCATGGCTCCCTCATCTACTCCTTCTCTGAATGTGCCTTTACAGGACCTCTGCGGC CCTTCTTCAGTCCTGGTTTCAATGATGGAGGAAAAAACACAGCCCCTCTAACCCTCTGTCCACTGAATAT TGGATCACAAGGATCCACTGACTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202088 protein sequence
Red=Cloning site Green=Tags(s) MASAARLTMMWEEVTCPICLDPFVEPVSIECGHSFCQECISQVGKGGGSVCPVCRQRFLLKNLRPNRQLA NMVNNLKEISQEAREGTQGERCAVHGERLHLFCEKDGKALCWVCAQSRKHRDHAMVPLEEAAQEYQEKLQ VALGELRRKQELAEKLEVEIAIKRADWKKTVETQKSRIHAEFVQQKNFLVEEEQRQLQELEKDEREQLRI LGEKEAKLAQQSQALQELISELDRRCHSSALELLQEVIIVLERSESWNLKDLDITSPELRSVCHVPGLKK MLRTCAVHITLDPDTANPWLILSEDRRQVRLGDTQQSIPGNEERFDSYPMVLGAQHFHSGKHYWEVDVTG KEAWDLGVCRDSVRRKGHFLLSSKSGFWTIWLWNKQKYEAGTYPQTPLHLQVPPCQVGIFLDYEAGMVSF YNITDHGSLIYSFSECAFTGPLRPFFSPGFNDGGKNTAPLTLCPLNIGSQGSTDY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003141 |
| ORF Size | 1425 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003141.4 |
| RefSeq Size | 1946 bp |
| RefSeq ORF | 1428 bp |
| Locus ID | 6737 |
| UniProt ID | P19474 |
| Domains | zf-B_box, RING, SPRY, PRY |
| Protein Families | Druggable Genome |
| Protein Pathways | Systemic lupus erythematosus |
| MW | 54.2 kDa |
| Gene Summary | This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
TRIB3 promotes MYC-associated lymphoma development through suppression of UBE3B-mediated MYC degradation
,Li, K;Wang, F;Yang, ZN;Zhang, TT;Yuan, YF;Zhao, CX;Yeerjiang, Z;Cui, B;Hua, F;Lv, XX;Zhang, XW;Yu, JJ;Liu, SS;Yu, JM;Shang, S;Xiao, Y;Hu, ZW;,
Nature communications
,PubMed ID 33298911
[TRIM21]
|
|
SFTS virus NSs interacts with TRIM21 to activate the p62-Keap1-Nrf2 pathway
,Choi, Y;Jiang, Z;Shin, WJ;Jung, JU;,
J. Virol.
,PubMed ID 31852783
[TRIM21]
|
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202088L1 | Lenti ORF clone of Human tripartite motif containing 21 (TRIM21), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC202088L2 | Lenti ORF clone of Human tripartite motif containing 21 (TRIM21), mGFP tagged |
CNY 7,888.00 |
|
| RC202088L3 | Lenti ORF clone of Human tripartite motif containing 21 (TRIM21), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC202088L4 | Lenti ORF clone of Human tripartite motif containing 21 (TRIM21), mGFP tagged |
CNY 5,890.00 |
|
| RG202088 | TRIM21 (tGFP-tagged) - Human tripartite motif containing 21 (TRIM21) |
CNY 7,088.00 |
|
| SC118185 | TRIM21 (untagged)-Human tripartite motif containing 21 (TRIM21) |
CNY 5,488.00 |
