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C17orf27 (RNF213) (NM_020954) Human Tagged ORF Clone
CAT＃: RC202317
RNF213 (Myc-DDK-tagged)-Human ring finger protein 213 (RNF213), transcript variant 2
ORF Plasmid: tGFP
"NM_020954" in other vectors (6)
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CNY 7,400.00
CNY 7,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ALO17; C17orf27; KIAA1618; MYMY2; MYSTR; NET57 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202317 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTGTCCTTCGTGCCAGCATGTCTCCAAGGAGGAAACCCCCAAGTTCTGCAGCCAGTGCGGAGAGA GGCTGCCTCCTGCAGCCCCCATAGCAGATTCTGAGAACAATAACTCCACAATGGCGTCGGCCTCGGAGGG TGAAATGGAGTGTGGGCAGGAGCTGAAGGAGGAAGGGGGCCCGTGCTTGTTCCCGGGCTCAGACAGTTGG CAAGAAAACCCCGAGGAGCCCTGTTCCAAAGCCTCCTGGACCGTCCAAGAAAGCAAAAAGAAGAAAAGGA AGAAGAAAAAGAAGGGGAACAAGTCCGCTTCCTCAGAGCTGGCTTCCTTGCCCCTTTCTCCTGCCAGCCC CTGTCACCTGACTTTGCTTTCAAACCCATGGCCTCAGGACACAGCCCTGCCCCACAGCCAAGCCCAGCAG AGTGGCCCCACTGGCCAGCCGAGCCAGCCCCCGGGCACAGCCACCACGCCACTGGAGGGTGACGGCCTCT CCGCGCCCACCGAGGTTGGCGACAGCCCCCTGCAGGCCCAGGCTTTGGGAGAGGCAGGAGTGGCCACAGG AAGTGAGGCTCAGAGCAGCCCGCAATTCCAGGACCACACGGAAGGGGAGGACCAGGACGCTTCCATCCCC TCTGGGGGCAGAGGCCTGTCCCAGGAGGGGACCGGTCCCCCCACCTCTGCTGGTGAAGGCCATTCTAGGA CTGAAGATGCTGCCCAGGAGCTCCTGTTGCCTGAGTCAAAAGGAGGCAGCTCTGAGCCCGGGACAGAACT GCAGACCACCGAGCAACAGGCAGGGGCCTCAGCCTCTACGGCAGTTGATGCTGTAGCTGAGCCAGCCAAT GCAGTTAAAGGGGCCGGGAAGGAAATGAAAGAGAAGACCCAGAGAATGAAACAGCCACCAGCAACCACTC CTCCTTTCAAAACACACTGCCAGGAAGCTGAGACCAAGACCAAGGACGAGACGGCTGCTGCTGAAGAAAA AGTCGGTAAAAATGAACAAGGGGAGCCTGAAGACCTCAAGAAGCCAGAGGGGAAGAACAGAAGTGCAGCT GCTGTGAAAAACGAGAAGGAGCAAAAAAACCAGGAAGCAGATGTCCAGGAAGTGAAGGCAAGCACGCTGA GCCCGGGTGGAGGAGTCACCGTGTTCTTCCACGCCATCATCTCTCTTCATTTCCCATTCAATCCTGACCT CCATAAAGTCTTCATCAGAGGAGGAGAAGAATTTGGGGAGTCAAAATGGGACAGCAATATCTGTGAGCTG CACTACACCAGAGACTTGGGTCATGACCGCGTTCTTGTTGAAGGCATTGTCTGCATTTCCAAGAAGCACC TAGATAAATACATTCCTTACAAGTACGTCATTTATAATGGGGAATCTTTTGAGTATGAGTTCATTTACAA GCACCAGCAGAAGAAGGGCGAGTACGTCAACCGCTGTCTGTTCATAAAATCTTCACTTCTGGGCTCAGGA GACTGGCATCAGTACTATGACATAGTTTATATGAAGCCTCATGGGAGACTCCAGAAAGTCATGAACCACA TCACAGACGGGCCGAGGAAGGACCTGGTGAAGGGGAAGCAGATTGCCGCTGCGCTCATGCTGGACAGCAC CTTCAGCATCCTGCAGACCTGGGACACCATCAACCTGAACAGCTTCTTCACCCAGTTCGAGCAGTTTTGC TTTGTCCTGCAACAGCCTATGATTTATGAAGGACAGGCACAGCTGTGGACCGATTTGCAGTACAGGGAGA AAGAGGTGAAGAGATACCTGTGGCAACATCTGAAAAAACACGTGGTACCATTGCCGGACGGAAAAAGCAC GGACTTTTTGCCTGTGGACTGCCCAGTGAGGAGTAAACTGAAAACAGGCCTGATTGTCCTTTTTGTAGTG GAAAAAATTGAGCTTTTATTAGAAGGCAGCCTGGACTGGTTGTGTCACCTCCTAACCTCAGATGCCAGCT CACCAGATGAGTTTCACCGTGACCTAAGCCACATCCTTGGGATACCTCAGAGCTGGCGGCTGTACCTGGT GAACCTGTGCCAAAGATGCATGGACACAAGGACGTACACCTGGCTGGGCGCCCTGCCTGTCCTGCACTGC TGTATGGAGCTGGCCCCGCGGCACAAGGATGCCTGGAGACAGCCTGAGGACACCTGGGCCGCTCTGGAGG GACTCTCCTTCTCACCGTTCCGGGAACAAATGCTAGATACGAGTTCCCTACTTCAGTTTATGAGAGAGAA GCAGCATTTGCTGAGCATAGACGAGCCTCTCTTCCGGTCCTGGTTTAGTCTGCTACCTCTGAGTCACCTG GTTATGTATATGGAAAACTTCATTGAGCACCTGGGTCGTTTTCCTGCTCATATCCTGGACTGTCTTTCAG GGATTTACTACCGGCTTCCGGGACTTGAGCAAGTCTTGAATACGCAGGATGTTCAGGATGTTCAGAACGT TCAGAACATTTTAGAAATGCTGTTGCGACTCCTGGACACTTACCGGGACAAGATTCCCGAGGAGGCCTTG TCACCATCCTACCTGACTGTGTGTCTGAAACTGCATGAAGCCATCTGCAGCAGCACAAAGCTACTTAAGT TTTACGAGCTGCCAGCCTTATCTGCCGAGATTGTCTGCAGAATGATTAGACTTCTATCTCTGGTGGATTC TGCAGGACAGAGAGATGAAACTGGAAATAATTCAGTCCAAACAGTCTTCCAAGGGACCCTTGCTGCTACG AAAAGGTGGCTCCGAGAAGTTTTTACAAAGAACATGCTCACATCTTCAGGTGCCTCATTCACATACGTCA AGGAAATTGAGGTCTGGAGGCGGCTGGTGGAAATCCAATTCCCCGCGGAGCATGGCTGGAAGGAGTCGTT GCTGGGAGACATGGAATGGAGGCTCACAAAGGAGGAACCCCTCTCCCAGATCACTGCCTACTGCAATAGT TGCTGGGACACCAAAGGCTTAGAGGACAGTGTGGCCAAGACCTTCGAGAAATGCATCATTGAAGCCGTGA GCTCAGCCTGCCAGGTGAACAATCTCTCCTCCTGGGAAACGGATTCGGGCTCACAGCTGTGTTCTGCCAT GACCCAGCTAAGGGCTATGAAGCACCCGCTGGGTCTCAGCTCCTCCGCTAACTCAGAGATTGGGAAGTGG GCACCCTCCTCCCTCGCCAAGGGCAATGGCGCTGAAATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202317 protein sequence
Red=Cloning site Green=Tags(s) MECPSCQHVSKEETPKFCSQCGERLPPAAPIADSENNNSTMASASEGEMECGQELKEEGGPCLFPGSDSW QENPEEPCSKASWTVQESKKKKRKKKKKGNKSASSELASLPLSPASPCHLTLLSNPWPQDTALPHSQAQQ SGPTGQPSQPPGTATTPLEGDGLSAPTEVGDSPLQAQALGEAGVATGSEAQSSPQFQDHTEGEDQDASIP SGGRGLSQEGTGPPTSAGEGHSRTEDAAQELLLPESKGGSSEPGTELQTTEQQAGASASTAVDAVAEPAN AVKGAGKEMKEKTQRMKQPPATTPPFKTHCQEAETKTKDETAAAEEKVGKNEQGEPEDLKKPEGKNRSAA AVKNEKEQKNQEADVQEVKASTLSPGGGVTVFFHAIISLHFPFNPDLHKVFIRGGEEFGESKWDSNICEL HYTRDLGHDRVLVEGIVCISKKHLDKYIPYKYVIYNGESFEYEFIYKHQQKKGEYVNRCLFIKSSLLGSG DWHQYYDIVYMKPHGRLQKVMNHITDGPRKDLVKGKQIAAALMLDSTFSILQTWDTINLNSFFTQFEQFC FVLQQPMIYEGQAQLWTDLQYREKEVKRYLWQHLKKHVVPLPDGKSTDFLPVDCPVRSKLKTGLIVLFVV EKIELLLEGSLDWLCHLLTSDASSPDEFHRDLSHILGIPQSWRLYLVNLCQRCMDTRTYTWLGALPVLHC CMELAPRHKDAWRQPEDTWAALEGLSFSPFREQMLDTSSLLQFMREKQHLLSIDEPLFRSWFSLLPLSHL VMYMENFIEHLGRFPAHILDCLSGIYYRLPGLEQVLNTQDVQDVQNVQNILEMLLRLLDTYRDKIPEEAL SPSYLTVCLKLHEAICSSTKLLKFYELPALSAEIVCRMIRLLSLVDSAGQRDETGNNSVQTVFQGTLAAT KRWLREVFTKNMLTSSGASFTYVKEIEVWRRLVEIQFPAEHGWKESLLGDMEWRLTKEEPLSQITAYCNS CWDTKGLEDSVAKTFEKCIIEAVSSACQVNNLSSWETDSGSQLCSAMTQLRAMKHPLGLSSSANSEIGKW APSSLAKGNGAEI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020954 |
| ORF Size | 3189 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020954.1 |
| RefSeq Size | 5337 bp |
| RefSeq ORF | 3192 bp |
| Locus ID | 57674 |
| UniProt ID | Q63HN8 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 118.4 kDa |
| Gene Summary | This gene encodes a protein containing a C3HC4-type RING finger domain, which is a specialized type of Zn-finger that binds two atoms of zinc and is thought to be involved in mediating protein-protein interactions. The protein also contains an AAA domain, which is associated with ATPase activity. This gene is a susceptibility gene for Moyamoya disease, a vascular disorder of intracranial arteries. This gene is also a translocation partner in anaplastic large cell lymphoma and inflammatory myofibroblastic tumor cases, where a t(2;17)(p23;q25) translocation has been identified with the anaplastic lymphoma kinase (ALK) gene on chromosome 2, and a t(8;17)(q24;q25) translocation has been identified with the MYC gene on chromosome 8. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202317L1 | Lenti ORF clone of Human ring finger protein 213 (RNF213), transcript variant 2, Myc-DDK-tagged |
CNY 9,800.00 |
|
| RC202317L2 | Lenti ORF clone of Human ring finger protein 213 (RNF213), transcript variant 2, mGFP tagged |
CNY 11,020.00 |
|
| RC202317L3 | Lenti ORF clone of Human ring finger protein 213 (RNF213), transcript variant 2, Myc-DDK-tagged |
CNY 11,020.00 |
|
| RC202317L4 | Lenti ORF clone of Human ring finger protein 213 (RNF213), transcript variant 2, mGFP tagged |
CNY 11,020.00 |
|
| RG202317 | RNF213 (tGFP-tagged) - Human ring finger protein 213 (RNF213), transcript variant 2 |
CNY 9,000.00 |
|
| SC126169 | RNF213 (untagged)-Human ring finger protein 213 (RNF213), transcript variant 2 |
CNY 10,450.00 |
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