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PIGQ (NM_148920) Human Tagged ORF Clone
CAT＃: RC202874
PIGQ (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class Q (PIGQ), transcript variant 1
ORF Plasmid: tGFP
"NM_148920" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | c407A10.1; DEE77; EIEE77; GPI1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202874 representing NM_148920
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCTCAAGGCCTTCTTCCCCACGTGCTGCGTCTCGACGGACAGCGGGCTGCTGGTGGGACGGTGGG TGCCGGAGCAGAGCAGCGCCGTGGTCCTGGCGGTCCTGCACTTTCCCTTCATCCCCATCCAGGTCAAGCA GCTCCTGGCCCAGGTGCGGCAGGCCAGCCAGGTGGGCGTGGCCGTGCTGGGCACCTGGTGCCACTGCCGG CAGGAGCCCGAGGAGAGCCTGGGCCGCTTCCTGGAGAGCCTGGGTGCTGTCTTCCCCCATGAGCCCTGGC TGCGGCTGTGCCGGGAGAGAGGCGGCACGTTCTGGAGCTGCGAGGCCACCCACCGGCAAGCGCCCACTGC CCCCGGTGCCCCTGGTGAGGACCAGGTCATGCTCATCTTCTATGACCAGCGCCAGGTGTTGCTGTCACAG CTACACCTGCCCACCGTCCTGCCCGACCGCCAGGCTGGAGCCACCACTGCCAGCACGGGGGGCCTGGCTG CCGTCTTCGACACGGTAGCACGCAGTGAGGTGCTCTTCCGCAGTGACCGCTTTGATGAGGGCCCCGTGCG GCTGAGCCACTGGCAGTCGGAGGGCGTGGAGGCCAGCATCCTCGCGGAGCTGGCCAGGCGAGCCTCGGGA CCCATTTGCCTGCTGTTGGCCAGCCTGCTGTCGCTGGTCTCAGCTGTCAGTGCCTGCCGAGTGTTCAAGC TCTGGCCCCTGTCCTTCCTCGGGAGCAAACTCTCCACGTGCGAACAGCTCCGGCACCGGCTGGAGCACCT CACGCTAATCTTCAGTACACGGAAGGCGGAGAACCCTGCCCAGCTGATGAGGAAGGCCAACACGGTGGCC TCTGTGCTGCTGGACGTGGCCCTGGGCCTCATGCTGCTGTCCTGGCTCCACGGGAGAAGCCGCATCGGGC ATCTGGCCGACGCCCTCGTTCCTGTGGCTGACCACGTGGCCGAGGAGCTCCAGCATCTGCTGCAGTGGCT GATGGGTGCTCCCGCCGGGCTCAAGATGAACCGTGCACTGGACCAGGTGCTGGGCCGCTTCTTCCTCTAC CACATCCACCTGTGGATCAGCTACATCCACCTCATGTCCCCCTTCGTGGAGCACATCCTTTGGCACGTGG GCCTCTCGGCCTGCCTGGGCCTGACGGTGGCCCTGTCCCTCCTCTCGGACATTATCGCCCTCCTCACCTT CCACATCTACTGCTTTTACGTCTATGGAGCCAGGCTGTACTGCCTGAAGATCCATGGCCTGTCCTCACTG TGGCGTCTGTTCCGGGGGAAGAAGTGGAACGTTCTGCGCCAGCGCGTGGACTCCTGTTCCTATGACCTGG ACCAGCTGTTCATCGGGACTCTGCTCTTCACCATCCTGCTCTTCCTCCTGCCTACCACAGCCCTGTACTA CCTGGTGTTCACCCTGCTCCGGCTCCTGGTGGTCGCCGTGCAGGGCCTGATCCATCTGCTCGTGGACCTC ATCAACTCCCTGCCGCTGTACTCACTGGGTCTTCGGCTCTGCCGGCCCTACAGGCTGGCGGATAAACCCA CTGCCCTACAGCCGCGTGGTGCACACCTACCGCCTCCCCAGCTGTGGCTGCCACCCCAAGCACTCCTGGG GCGCCCTGTGCCGCAAGCTGTTCCTTGGGGAGCTCATCTACCCCTGGAGGCAGAGAGGGGACAAGCAGGA CTGAGGGAACTGCTGGCTCGCCTGGCACCACCACACGGCCACAGCCAGCCATCTGCTCTGCCAGGGTGGC ACCAGCTCAGCTGGCGCATGTCCTGTGCTTTGTGGACGCTGCTGTGTGCTCCTGAACACGGCAGGCCCTG CTATCACACCTTGGGCTTGGAGGTCATTGGGAGTGAGCAGATGTGGGGGTGGCCAGCCAGGCTGGCCGCA CTCCATCACTGGCACTGCCTGCCTTGGGACCCGCTTCCCACCTGCTGCGGTCACCATGGTGGCGAGCACA GCAACCCCAGGTGTCCAGAGCACTGCCCCATGCCCACCCTGTGTACCCAGGTCCAGAGGGTCCGTCCACC ACAGCAGCCCCAGGTGGAGGGCTGGTCTCCCTGGGGGCTCCCCAGTGGCTCTGCCCTGGCTGTGGGGGTG GAGGGACCTTGCCAGGATGAACCCCCCAGTCCCAGGCACCCTCTAGCTCCCTCAGCCGAACAGCACCCTG CATCTGGGGGATTGAAGCAGTCGCTGACCCCCGTCCCCAGCGGGCCCGGGCCCTCACTCCCTGAACCACA CGGGGTTTATTTGCGGATGTTCCCTGGAGAGGTCGCTTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202874 representing NM_148920
Red=Cloning site Green=Tags(s) MVLKAFFPTCCVSTDSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGVAVLGTWCHCR QEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGAPGEDQVMLIFYDQRQVLLSQ LHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDRFDEGPVRLSHWQSEGVEASILAELARRASG PICLLLASLLSLVSAVSACRVFKLWPLSFLGSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMRKANTVA SVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLY HIHLWISYIHLMSPFVEHILWHVGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSL WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDL INSLPLYSLGLRLCRPYRLADKPTALQPRGAHLPPPQLWLPPQALLGRPVPQAVPWGAHLPLEAERGQAG LRELLARLAPPHGHSQPSALPGWHQLSWRMSCALWTLLCAPEHGRPCYHTLGLEVIGSEQMWGWPARLAA LHHWHCLPWDPLPTCCGHHGGEHSNPRCPEHCPMPTLCTQVQRVRPPQQPQVEGWSPWGLPSGSALAVGV EGPCQDEPPSPRHPLAPSAEQHPASGGLKQSLTPVPSGPGPSLPEPHGVYLRMFPGEVAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_148920 |
| ORF Size | 2280 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_148920.3 |
| RefSeq Size | 2878 bp |
| RefSeq ORF | 2283 bp |
| Locus ID | 9091 |
| UniProt ID | Q9BRB3 |
| Domains | Gpi1 |
| Protein Families | Transmembrane |
| Protein Pathways | Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways |
| MW | 83.9 kDa |
| Gene Summary | This gene is involved in the first step in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This gene encodes a N-acetylglucosaminyl transferase component that is part of the complex that catalyzes transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to phosphatidylinositol (PI). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202874L3 | Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class Q (PIGQ), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202874L4 | Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class Q (PIGQ), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG202874 | PIGQ (tGFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class Q (PIGQ), transcript variant 1 |
CNY 4,370.00 |
|
| SC319169 | PIGQ (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class Q (PIGQ), transcript variant 1 |
CNY 2,950.00 |
