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Alanine Transaminase (GPT) (NM_005309) Human Tagged ORF Clone
CAT＃: RC203756
GPT (Myc-DDK-tagged)-Human glutamic-pyruvate transaminase (alanine aminotransferase) (GPT)
ORF Plasmid: tGFP
"NM_005309" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AAT1; ALT1; GPT1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203756 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCGAGCACAGGTGACCGGAGCCAGGCGGTGAGGCATGGACTGAGGGCGAAGGTGCTGACGCTGG ACGGCATGAACCCGCGTGTGCGGAGAGTGGAGTACGCAGTGCGTGGCCCCATAGTGCAGCGAGCCTTGGA GCTGGAGCAGGAGCTGCGCCAGGGTGTGAAGAAGCCTTTCACCGAGGTCATCCGTGCCAACATCGGGGAC GCACAGGCTATGGGGCAGAGGCCCATCACCTTCCTGCGCCAGGTCTTGGCCCTCTGTGTTAACCCTGATC TTCTGAGCAGCCCCAACTTCCCTGACGATGCCAAGAAAAGGGCGGAGCGCATCTTGCAGGCGTGTGGGGG CCACAGTCTGGGGGCCTACAGCGTCAGCTCCGGCATCCAGCTGATCCGGGAGGACGTGGCGCGGTACATT GAGAGGCGTGACGGAGGCATCCCTGCGGACCCCAACAACGTCTTCCTGTCCACAGGGGCCAGCGATGCCA TCGTGACGGTGCTGAAGCTGCTGGTGGCCGGCGAGGGCCACACACGCACGGGTGTGCTCATCCCCATCCC CCAGTACCCACTCTACTCGGCCACGCTGGCAGAGCTGGGCGCAGTGCAGGTGGATTACTACCTGGACGAG GAGCGTGCCTGGGCGCTGGACGTGGCCGAGCTTCACCGTGCACTGGGCCAGGCGCGTGACCACTGCCGCC CTCGTGCGCTCTGTGTCATCAACCCTGGCAACCCCACCGGGCAGGTGCAGACCCGCGAGTGCATCGAGGC CGTGATCCGCTTCGCCTTCGAAGAGCGGCTCTTTCTGCTGGCGGACGAGGTGTACCAGGACAACGTGTAC GCCGCGGGTTCGCAGTTCCACTCATTCAAGAAGGTGCTCATGGAGATGGGGCCGCCCTACGCCGGGCAGC AGGAGCTTGCCTCCTTCCACTCCACCTCCAAGGGCTACATGGGCGAGTGCGGGTTCCGCGGCGGCTATGT GGAGGTGGTGAACATGGACGCTGCAGTGCAGCAGCAGATGCTGAAGCTGATGAGTGTGCGGCTGTGCCCG CCGGTGCCAGGACAGGCCCTGCTGGACCTGGTGGTCAGCCCGCCCGCGCCCACCGACCCCTCCTTTGCGC AGTTCCAGGCTGAGAAGCAGGCAGTGCTGGCAGAGCTGGCGGCCAAGGCCAAGCTCACCGAGCAGGTCTT CAATGAGGCTCCTGGCATCAGCTGCAACCCAGTGCAGGGCGCCATGTACTCCTTCCCGCGCGTGCAGCTG CCCCCGCGGGCGGTGGAGCGCGCTCAGGAGCTGGGCCTGGCCCCCGATATGTTCTTCTGCCTGCGCCTCC TGGAGGAGACCGGCATCTGCGTGGTGCCAGGGAGCGGCTTTGGGCAGCGGGAAGGCACCTACCACTTCCG GATGACCATTCTGCCCCCCTTGGAGAAACTGCGGCTGCTGCTGGAGAAGCTGAGCAGGTTCCATGCCAAG TTCACCCTCGAGTACTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203756 protein sequence
Red=Cloning site Green=Tags(s) MASSTGDRSQAVRHGLRAKVLTLDGMNPRVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGD AQAMGQRPITFLRQVLALCVNPDLLSSPNFPDDAKKRAERILQACGGHSLGAYSVSSGIQLIREDVARYI ERRDGGIPADPNNVFLSTGASDAIVTVLKLLVAGEGHTRTGVLIPIPQYPLYSATLAELGAVQVDYYLDE ERAWALDVAELHRALGQARDHCRPRALCVINPGNPTGQVQTRECIEAVIRFAFEERLFLLADEVYQDNVY AAGSQFHSFKKVLMEMGPPYAGQQELASFHSTSKGYMGECGFRGGYVEVVNMDAAVQQQMLKLMSVRLCP PVPGQALLDLVVSPPAPTDPSFAQFQAEKQAVLAELAAKAKLTEQVFNEAPGISCNPVQGAMYSFPRVQL PPRAVERAQELGLAPDMFFCLRLLEETGICVVPGSGFGQREGTYHFRMTILPPLEKLRLLLEKLSRFHAK FTLEYS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005309 |
| ORF Size | 1488 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005309.3 |
| RefSeq Size | 1896 bp |
| RefSeq ORF | 1491 bp |
| Locus ID | 2875 |
| UniProt ID | P24298 |
| Protein Families | Druggable Genome |
| Protein Pathways | Alanine, aspartate and glutamate metabolism, Metabolic pathways |
| MW | 54.6 kDa |
| Gene Summary | This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Liver alanine catabolism promotes skeletal muscle atrophy and hyperglycaemia in type 2 diabetes
,Okun, JG;Rusu, PM;Chan, AY;Wu, Y;Yap, YW;Sharkie, T;Schumacher, J;Schmidt, KV;Roberts-Thomson, KM;Russell, RD;Zota, A;Hille, S;Jungmann, A;Maggi, L;Lee, Y;Bl?her, M;Herzig, S;Keske, MA;Heikenwalder, M;M?ller, OJ;Rose, AJ;,
Nature metabolism
,PubMed ID 33758419
[Alanine Transaminase]
|
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An endocrine-hepato-muscular metabolic cycle links skeletal muscle atrophy and hyperglycemia in type 2 diabetes
,Okun, JG;Rusu, PM;Chan, AY;Yap, YW;Sharkie, T;,
bioRxiv
[Alanine Transaminase]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203756L3 | Lenti ORF clone of Human glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203756L4 | Lenti ORF clone of Human glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), mGFP tagged |
CNY 5,890.00 |
|
| RG203756 | GPT (tGFP-tagged) - Human glutamic-pyruvate transaminase (alanine aminotransferase) (GPT) |
CNY 4,370.00 |
|
| SC122706 | GPT (untagged)-Human glutamic-pyruvate transaminase (alanine aminotransferase) (GPT) |
CNY 2,950.00 |
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