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DPM2 (NM_003863) Human Tagged ORF Clone
CAT＃: RC203919
DPM2 (Myc-DDK-tagged)-Human dolichyl-phosphate mannosyltransferase polypeptide 2, regulatory subunit (DPM2)
ORF Plasmid: tGFP
"NM_003863" in other vectors (6)
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CNY 1,200.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CDG1U |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203919 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACGGGGACAGACCAGGTGGTGGGACTCGGCCTCGTCGCCGTTAGCCTGATCATCTTCACCTACT ACACCGCCTGGGTGATTCTCTTGCCATTCATCGACAGTCAGCATGTCATCCACAAGTATTTCCTGCCCCG AGCCTATGCTGTCGCCATCCCACTGGCTGCAGGCCTCCTGCTGCTCCTGTTTGTGGGACTGTTCATCTCC TACGTGATGCTGAAGAGCAAGAGAGTGACCAAGAAGGCTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203919 protein sequence
Red=Cloning site Green=Tags(s) MATGTDQVVGLGLVAVSLIIFTYYTAWVILLPFIDSQHVIHKYFLPRAYAVAIPLAAGLLLLLFVGLFIS YVMLKSKRVTKKAQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003863 |
| ORF Size | 252 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003863.4 |
| RefSeq Size | 1561 bp |
| RefSeq ORF | 255 bp |
| Locus ID | 8818 |
| UniProt ID | O94777 |
| Protein Families | Transmembrane |
| Protein Pathways | Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways, N-Glycan biosynthesis |
| MW | 9.3 kDa |
| Gene Summary | Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. The protein encoded by this gene is a hydrophobic protein that contains 2 predicted transmembrane domains and a putative ER localization signal near the C terminus. This protein associates with DPM1 in vivo and is required for the ER localization and stable expression of DPM1 and also enhances the binding of dolichol-phosphate to DPM1. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203919L1 | Lenti ORF clone of Human dolichyl-phosphate mannosyltransferase polypeptide 2, regulatory subunit (DPM2), Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC203919L2 | Lenti ORF clone of Human dolichyl-phosphate mannosyltransferase polypeptide 2, regulatory subunit (DPM2), mGFP tagged |
CNY 5,890.00 |
|
| RC203919L3 | Lenti ORF clone of Human dolichyl-phosphate mannosyltransferase polypeptide 2, regulatory subunit (DPM2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203919L4 | Lenti ORF clone of Human dolichyl-phosphate mannosyltransferase polypeptide 2, regulatory subunit (DPM2), mGFP tagged |
CNY 5,890.00 |
|
| RG203919 | DPM2 (tGFP-tagged) - Human dolichyl-phosphate mannosyltransferase polypeptide 2, regulatory subunit (DPM2) |
CNY 2,800.00 |
|
| SC127744 | DPM2 (untagged)-Human dolichyl-phosphate mannosyltransferase polypeptide 2, regulatory subunit (DPM2) |
CNY 1,200.00 |
