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HAUS1 (NM_138443) Human Tagged ORF Clone
CAT＃: RC204226
HAUS1 (Myc-DDK-tagged)-Human HAUS augmin-like complex, subunit 1 (HAUS1), transcript variant 1
ORF Plasmid: tGFP
"NM_138443" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CCDC5; HEI-C; HEIC; HsT1461 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204226 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGCAGGAGGAGAGAGAAACGCAGGTTGCTGCGTGGTTAAAAAAAATATTTGGAGATCATCCTA TTCCACAGTATGAGGTGAACCCACGGACCACAGAGATTTTACATCACCTTTCAGAACGCAACAGGGTCCG GGACAGGGATGTCTACCTGGTAATAGAGGACTTGAAGCAGAAAGCAAGTGAATACGAGTCAGAAGCCAAG TATCTTCAAGACCTTCTCATGGAGAGTGTGAATTTTTCCCCCGCCAATCTCTCTAGCACTGGTTCCAGGT ATCTGAATGCTTTGGTTGACAGTGCGGTGGCCCTTGAAACAAAGGATACCTCGCTAGCTAGTTTTATCCC TGCAGTGAATGATTTGACCTCTGATCTCTTTCGTACCAAATCCAAAAGTGAAGAAATCAAGATTGAACTG GAAAAACTTGAAAAAAATTTAACTGCAACTTTAGTATTAGAAAAATGTCTACAAGAGGATGTCAAGAAAG CAGAGTTGCATCTGTCTACAGAAAGGGCCAAAGTTGATAATCGTCGTCAGAACATGGACTTTCTAAAAGC AAAGTCAGAGGAATTCAGATTTGGAATCAAGGCTGCAGAGGAGCAACTTTCAGCCAGAGGCATGGATGCT TCTCTGTCTCATCAGTCCTTAGTAGCACTATCAGAGAAACTGGCAAGATTAAAGCAACAGACTATACCTT TGAAGAAAAAATTGGAGTCCTATTTAGACTTAATGCCGAATCCGTCTCTTGCTCAAGTGAAAATTGAAGA AGCAAAGCGAGAACTAGATAGCATTGAAGCTGAACTTACAAGAAGAGTAGACATGATGGAACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204226 protein sequence
Red=Cloning site Green=Tags(s) MEPQEERETQVAAWLKKIFGDHPIPQYEVNPRTTEILHHLSERNRVRDRDVYLVIEDLKQKASEYESEAK YLQDLLMESVNFSPANLSSTGSRYLNALVDSAVALETKDTSLASFIPAVNDLTSDLFRTKSKSEEIKIEL EKLEKNLTATLVLEKCLQEDVKKAELHLSTERAKVDNRRQNMDFLKAKSEEFRFGIKAAEEQLSARGMDA SLSHQSLVALSEKLARLKQQTIPLKKKLESYLDLMPNPSLAQVKIEEAKRELDSIEAELTRRVDMMEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138443 |
| ORF Size | 834 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138443.4 |
| RefSeq Size | 1145 bp |
| RefSeq ORF | 837 bp |
| Locus ID | 115106 |
| UniProt ID | Q96CS2 |
| Protein Families | Stem cell - Pluripotency |
| MW | 31.9 kDa |
| Gene Summary | HAUS1 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]).[supplied by OMIM, Jun 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204226L3 | Lenti ORF clone of Human HAUS augmin-like complex, subunit 1 (HAUS1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204226L4 | Lenti ORF clone of Human HAUS augmin-like complex, subunit 1 (HAUS1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG204226 | HAUS1 (tGFP-tagged) - Human HAUS augmin-like complex, subunit 1 (HAUS1), transcript variant 1 |
CNY 4,370.00 |
|
| SC123138 | HAUS1 (untagged)-Human HAUS augmin-like complex, subunit 1 (HAUS1), transcript variant 1 |
CNY 2,400.00 |
