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TFIIB (GTF2B) (NM_001514) Human Tagged ORF Clone
CAT＃: RC204607
GTF2B (Myc-DDK-tagged)-Human general transcription factor IIB (GTF2B)
ORF Plasmid: tGFP
"NM_001514" in other vectors (5)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | TF2B; TFIIB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204607 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCTACCAGCCGTTTGGATGCTCTTCCAAGAGTCACATGTCCAAACCATCCAGATGCGATTTTAG TGGAGGACTACAGAGCCGGTGATATGATCTGTCCTGAATGTGGCTTGGTTGTAGGTGACCGGGTTATTGA TGTGGGATCTGAATGGCGAACTTTCAGCAATGACAAAGCAACAAAAGATCCATCTCGAGTTGGAGATTCT CAGAATCCTCTTCTGAGTGATGGAGATTTGTCTACCATGATTGGCAAGGGCACAGGAGCTGCAAGTTTTG ACGAATTTGGCAATTCTAAGTACCAGAATCGGAGAACAATGAGCAGTTCTGATCGGGCAATGATGAATGC ATTCAAAGAAATCACTACCATGGCAGACAGAATCAATCTACCTCGAAATATAGTTGATCGAACAAATAAT TTATTCAAGCAAGTATATGAACAGAAGAGCCTGAAGGGAAGAGCTAATGATGCTATAGCTTCTGCTTGTC TCTATATTGCCTGTAGACAAGAAGGGGTTCCTAGGACATTTAAAGAAATATGTGCCGTATCACGAATTTC TAAGAAAGAAATTGGTCGGTGTTTTAAACTTATTTTGAAAGCGCTAGAAACCAGTGTGGATTTGATTACA ACTGGGGACTTCATGTCCAGGTTCTGTTCCAACCTTTGTCTTCCTAAACAAGTACAGATGGCAGCTACAC ATATAGCCCGTAAAGCTGTGGAATTGGACTTGGTTCCTGGGAGGAGCCCCATCTCTGTGGCAGCGGCAGC TATTTACATGGCCTCACAGGCATCAGCTGAAAAGAGGACCCAAAAAGAAATTGGAGATATTGCTGGTGTT GCTGATGTTACAATCAGACAGTCCTATAGACTGATCTATCCTCGAGCCCCAGATCTGTTTCCTACAGACT TCAAATTTGACACCCCAGTGGACAAACTACCACAGCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204607 protein sequence
Red=Cloning site Green=Tags(s) MASTSRLDALPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDS QNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNN LFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLIT TGDFMSRFCSNLCLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGV ADVTIRQSYRLIYPRAPDLFPTDFKFDTPVDKLPQL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001514 |
| ORF Size | 948 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001514.5 |
| RefSeq Size | 1651 bp |
| RefSeq ORF | 951 bp |
| Locus ID | 2959 |
| UniProt ID | Q00403 |
| Domains | transcript_fac2, CYCLIN |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Basal transcription factors |
| MW | 34.8 kDa |
| Gene Summary | This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204607L3 | Lenti ORF clone of Human general transcription factor IIB (GTF2B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204607L4 | Lenti ORF clone of Human general transcription factor IIB (GTF2B), mGFP tagged |
CNY 5,890.00 |
|
| RG204607 | GTF2B (tGFP-tagged) - Human general transcription factor IIB (GTF2B) |
CNY 4,000.00 |
|
| SC110914 | GTF2B (untagged)-Human general transcription factor IIB (GTF2B) |
CNY 2,400.00 |
|
| SC321111 | GTF2B (untagged)-Human general transcription factor IIB (GTF2B) |
CNY 2,400.00 |
