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VPS36 (NM_016075) Human Tagged ORF Clone
CAT＃: RC206006
VPS36 (Myc-DDK-tagged)-Human vacuolar protein sorting 36 homolog (S. cerevisiae) (VPS36)
ORF Plasmid: tGFP
"NM_016075" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C13orf9; CGI-145; EAP45 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206006 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCGCTTCGTTTGGACCAGCGGCCTCCTGGAGATCAACGAGACCCTGGTGATCCAGCAGCGCGGGG TGCGAATCTACGATGGCGAGGAGAAGATAAAATTTGATGCTGGGACTCTCCTTCTTAGTACACACCGACT GATTTGGAGAGATCAGAAAAATCATGAGTGTTGCATGGCCATTCTCCTTTCCCAAATTGTGTTCATTGAA GAACAGGCGGCTGGAATTGGGAAGAGTGCCAAAATAGTGGTTCATCTTCACCCAGCTCCTCCTAACAAAG AACCTGGCCCATTCCAGAGTAGTAAGAACTCCTACATCAAACTCTCCTTCAAAGAACATGGCCAGATTGA GTTTTACAGGCGTTTATCAGAGGAAATGACACAAAGAAGATGGGAGAATATGCCAGTTTCCCAGTCATTA CAAACAAATAGAGGACCCCAGCCAGGAAGAATAAGGGCTGTAGGAATTGTAGGTATTGAAAGGAAACTGG AAGAAAAAAGAAAAGAAACTGACAAAAACATTTCTGAGGCCTTTGAAGACCTCAGCAAACTAATGATCAA GGCTAAGGAAATGGTGGAATTATCAAAATCAATTGCTAATAAAATTAAAGACAAACAAGGTGACATCACA GAAGATGAGACCATCAGGTTTAAATCCTACTTGCTGAGCATGGGAATAGCTAACCCAGTTACCAGAGAAA CCTACGGCTCAGGCACACAGTACCACATGCAGCTGGCCAAACAACTGGCTGGAATATTGCAGGTGCCTTT AGAGGAACGAGGGGGAATAATGTCACTCACGGAGGTGTACTGCTTAGTAAACCGAGCTCGAGGAATGGAA TTGCTCTCACCAGAAGATTTAGTGAATGCGTGCAAGATGCTGGAAGCACTGAAATTACCTCTCAGGCTCC GTGTGTTTGACAGTGGCGTCATGGTAATTGAGCTTCAGTCTCACAAGGAAGAGGAAATGGTGGCCTCGGC CCTGGAGACAGTTTCAGAAAAGGGATCCCTAACATCAGAAGAGTTTGCTAAGCTTGTGGGAATGTCTGTC CTCCTAGCCAAAGAAAGGTTGCTGCTTGCAGAGAAGATGGGCCATCTTTGCCGTGATGACTCAGTGGAAG GCCTGCGTTTTTACCCAAATTTATTTATGACACAGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206006 protein sequence
Red=Cloning site Green=Tags(s) MDRFVWTSGLLEINETLVIQQRGVRIYDGEEKIKFDAGTLLLSTHRLIWRDQKNHECCMAILLSQIVFIE EQAAGIGKSAKIVVHLHPAPPNKEPGPFQSSKNSYIKLSFKEHGQIEFYRRLSEEMTQRRWENMPVSQSL QTNRGPQPGRIRAVGIVGIERKLEEKRKETDKNISEAFEDLSKLMIKAKEMVELSKSIANKIKDKQGDIT EDETIRFKSYLLSMGIANPVTRETYGSGTQYHMQLAKQLAGILQVPLEERGGIMSLTEVYCLVNRARGME LLSPEDLVNACKMLEALKLPLRLRVFDSGVMVIELQSHKEEEMVASALETVSEKGSLTSEEFAKLVGMSV LLAKERLLLAEKMGHLCRDDSVEGLRFYPNLFMTQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016075 |
| ORF Size | 1158 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016075.4 |
| RefSeq Size | 4473 bp |
| RefSeq ORF | 1161 bp |
| Locus ID | 51028 |
| UniProt ID | Q86VN1 |
| Domains | Vps36 |
| Protein Families | Transcription Factors |
| Protein Pathways | Endocytosis |
| MW | 43.8 kDa |
| Gene Summary | This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A similar protein complex in rat is associated with RNA polymerase elongation factor II. [provided by RefSeq, Aug 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206006L1 | Lenti ORF clone of Human vacuolar protein sorting 36 homolog (S. cerevisiae) (VPS36), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206006L2 | Lenti ORF clone of Human vacuolar protein sorting 36 homolog (S. cerevisiae) (VPS36), mGFP tagged |
CNY 5,890.00 |
|
| RC206006L3 | Lenti ORF clone of Human vacuolar protein sorting 36 homolog (S. cerevisiae) (VPS36), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206006L4 | Lenti ORF clone of Human vacuolar protein sorting 36 homolog (S. cerevisiae) (VPS36), mGFP tagged |
CNY 5,890.00 |
|
| RG206006 | VPS36 (tGFP-tagged) - Human vacuolar protein sorting 36 homolog (S. cerevisiae) (VPS36) |
CNY 4,370.00 |
|
| SC114437 | VPS36 (untagged)-Human vacuolar protein sorting 36 homolog (S. cerevisiae) (VPS36) |
CNY 3,656.00 |
