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Ceramide glucosyltransferase (UGCG) (NM_003358) Human Tagged ORF Clone
CAT＃: RC206288
UGCG (Myc-DDK-tagged)-Human UDP-glucose ceramide glucosyltransferase (UGCG)
ORF Plasmid: tGFP
"NM_003358" in other vectors (6)
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CNY 5,488.00
| Cited in 4 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GCS; GLCT1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206288 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTGCTGGACCTGGCCTTGGAGGGAATGGCCGTCTTCGGGTTCGTCCTCTTCTTGGTGCTGTGGC TGATGCATTTCATGGCTATCATCTACACCCGATTACACCTCAACAAGAAGGCAACTGACAAACAGCCTTA TAGCAAGCTCCCAGGTGTCTCTCTTCTGAAACCACTGAAAGGGGTAGATCCTAACTTAATCAACAACCTG GAAACATTCTTTGAATTGGATTATCCCAAATATGAAGTGCTCCTTTGTGTACAAGATCATGATGATCCAG CCATTGATGTATGTAAGAAGCTTCTTGGAAAATATCCAAATGTTGATGCTAGATTGTTTATAGGTGGCAA AAAAGTTGGCATTAATCCTAAAATTAATAATTTAATGCCAGGATATGAAGTTGCAAAGTATGATCTTATA TGGATTTGTGATAGTGGAATAAGAGTAATTCCAGATACACTTACTGACATGGTGAATCAAATGACAGAAA AAGTAGGCTTGGTTCACGGGCTGCCTTACGTAGCAGACAGACAGGGCTTTGCTGCCACCTTAGAGCAGGT ATATTTTGGAACTTCACATCCAAGATACTATATCTCTGCCAATGTAACTGGTTTCAAATGTGTGACAGGA ATGTCTTGTTTAATGAGAAAAGATGTGTTGGATCAAGCAGGAGGACTTATAGCTTTTGCTCAGTACATTG CCGAAGATTACTTTATGGCCAAAGCGATAGCTGACCGAGGTTGGAGGTTTGCAATGTCCACTCAAGTTGC AATGCAAAACTCTGGCTCATATTCAATTTCTCAGTTTCAATCCAGAATGATCAGGTGGACCAAACTACGA ATTAACATGCTTCCTGCTACAATAATTTGTGAGCCAATTTCAGAATGCTTTGTTGCCAGTTTAATTATTG GATGGGCAGCCCACCATGTGTTCAGATGGGATATTATGGTATTTTTCATGTGTCATTGCCTGGCATGGTT TATATTTGACTACATTCAACTCAGGGGTGTCCAGGGTGGCACACTGTGTTTTTCAAAACTTGATTATGCA GTCGCCTGGTTCATCCGCGAATCCATGACAATATACATTTTTTTGTCTGCATTATGGGACCCAACTATAA GCTGGAGAACTGGTCGCTACAGATTACGCTGTGGGGGTACAGCAGAGGAAATCCTAGATGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206288 protein sequence
Red=Cloning site Green=Tags(s) MALLDLALEGMAVFGFVLFLVLWLMHFMAIIYTRLHLNKKATDKQPYSKLPGVSLLKPLKGVDPNLINNL ETFFELDYPKYEVLLCVQDHDDPAIDVCKKLLGKYPNVDARLFIGGKKVGINPKINNLMPGYEVAKYDLI WICDSGIRVIPDTLTDMVNQMTEKVGLVHGLPYVADRQGFAATLEQVYFGTSHPRYYISANVTGFKCVTG MSCLMRKDVLDQAGGLIAFAQYIAEDYFMAKAIADRGWRFAMSTQVAMQNSGSYSISQFQSRMIRWTKLR INMLPATIICEPISECFVASLIIGWAAHHVFRWDIMVFFMCHCLAWFIFDYIQLRGVQGGTLCFSKLDYA VAWFIRESMTIYIFLSALWDPTISWRTGRYRLRCGGTAEEILDV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003358 |
| ORF Size | 1182 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003358.3 |
| RefSeq Size | 1637 bp |
| RefSeq ORF | 1185 bp |
| Locus ID | 7357 |
| UniProt ID | Q16739 |
| Domains | Glycos_transf_2 |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, Sphingolipid metabolism |
| MW | 44.9 kDa |
| Gene Summary | This gene encodes an enzyme that catalyzes the first glycosylation step in the biosynthesis of glycosphingolipids, which are membrane components containing lipid and sugar moieties. The product of this reaction is glucosylceramide, which is the core structure of many glycosphingolipids. [provided by RefSeq, Dec 2014] |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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UGCG overexpression leads to increased glycolysis and increased oxidative phosphorylation of breast cancer cells
,Schömel, N;Gruber, L;Alexopoulos, SJ;Trautmann, S;Olzomer, EM;Byrne, FL;Hoehn, KL;Gurke, R;Thomas, D;Ferreirós, N;Geisslinger, G;Wegner, MS;,
Sci Rep
,PubMed ID 32424263
[Ceramide glucosyltransferase]
|
|
UGCG influences glutamine metabolism of breast cancer cells
,Schömel, N;Hancock, SE;Gruber, L;Olzomer, EM;Byrne, FL;Shah, D;Hoehn, KL;Turner, N;Grösch, S;Geisslinger, G;Wegner, MS;,
Sci Rep
,PubMed ID 31666638
[Ceramide glucosyltransferase]
|
|
UDP-glucose ceramide glucosyltransferase activates AKT, promoted proliferation, and doxorubicin resistance in breast cancer cells
,Wegner, MS;Schömel, N;Gruber, L;Örtel, SB;Kjellberg, MA;Mattjus, P;Kurz, J;Trautmann, S;Peng, B;Wegner, M;Kaulich, M;Ahrends, R;Geisslinger, G;Grösch, S;,
Cell. Mol. Life Sci.
,PubMed ID 29549423
[Ceramide glucosyltransferase]
|
|
The UDP-glucose ceramide glycosyltransferase (UGCG) and the link to multidrug resistance protein 1 (MDR1)
,Wegner, MS;Gruber, L;Mattjus, P;Geisslinger, G;Grösch, S;,
BMC Cancer
,PubMed ID 29409484
[Ceramide glucosyltransferase]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206288L1 | Lenti ORF clone of Human UDP-glucose ceramide glucosyltransferase (UGCG), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC206288L2 | Lenti ORF clone of Human UDP-glucose ceramide glucosyltransferase (UGCG), mGFP tagged |
CNY 7,888.00 |
|
| RC206288L3 | Lenti ORF clone of Human UDP-glucose ceramide glucosyltransferase (UGCG), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC206288L4 | Lenti ORF clone of Human UDP-glucose ceramide glucosyltransferase (UGCG), mGFP tagged |
CNY 7,888.00 |
|
| RG206288 | UGCG (tGFP-tagged) - Human UDP-glucose ceramide glucosyltransferase (UGCG) |
CNY 7,088.00 |
|
| SC118052 | UGCG (untagged)-Human UDP-glucose ceramide glucosyltransferase (UGCG) |
CNY 5,488.00 |
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