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PDILT (NM_174924) Human Tagged ORF Clone
CAT＃: RC206717
PDILT (Myc-DDK-tagged)-Human protein disulfide isomerase-like, testis expressed (PDILT)
ORF Plasmid: tGFP
"NM_174924" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PDIA7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206717 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCTACTCTGGATGCCCCTGCTGCTGGTGGCCGCTTGTGTCTCTGCTGTCCACAGCTCACCAGAGG TTAACGCCGGTGTTTCCAGCATCCACATAACCAAGCCTGTGCACATCCTGGAGGAACGCAGTCTCCTAGT GCTAACGCCCGCTGGCCTGACCCAGATGCTGAACCAGACCCGCTTCCTCATGGTGCTTTTCCACAACCCA TCCTCAAAGCAATCCAGGAACTTGGCGGAAGAGCTGGGCAAAGCTGTGGAGATCATGGGCAAAGGCAAGA ATGGGATCGGCTTTGGCAAAGTGGACATTACCATAGAGAAGGAGCTTCAGCAGGAGTTTGGGATTACCAA GGCCCCGGAGTTGAAGCTGTTTTTTGAGGGCAACAGGTCAGAGCCCATCAGCTGCAAAGGAGTGGTTGAA TCTGCTGCCTTAGTCGTTTGGTTGAGACGACAAATTAGCCAGAAAGCATTTTTGTTCAACAGCAGCGAGC AGGTGGCAGAGTTTGTGATATCCAGGCCCTTGGTCATCGTTGGCTTCTTCCAGGATTTAGAGGAAGAAGT AGCAGAGTTGTTCTATGATGTGATCAAAGACTTTCCAGAGCTAACGTTTGGAGTCATAACGATTGGCAAT GTCATTGGGCGTTTCCACGTCACCCTTGACAGCGTCCTGGTGTTCAAAAAGGGAAAAATTGTGAACCGCC AAAAGCTTATTAATGACAGTACCAACAAACAGGAACTCAATCGTGTCATAAAACAGCACCTTACAGATTT TGTGATCGAATACAACACTGAGAATAAGGATCTGATTTCCGAGTTGCACATCATGAGTCACATGCTGCTG TTTGTCTCCAAAAGCTCCGAGTCATATGGTATCATAATTCAGCATTATAAGCTGGCATCAAAGGAATTCC AAAACAAGATCCTTTTCATCCTTGTGGATGCAGACGAACCCAGAAATGGACGTGTCTTCAAGTACTTCCG GGTCACAGAGGTCGATATCCCATCCGTCCAAATCCTAAACTTGAGCTCTGACGCCAGGTACAAAATGCCT TCAGATGACATAACCTACGAAAGCCTCAAGAAATTTGGCCGCAGCTTCCTGAGTAAAAATGCCACAAAAC ATCAATCCAGTGAAGAGATTCCAAAATACTGGGACCAGGGACTGGTTAAGCAGCTCGTGGGGAAGAACTT CAACGTAGTCGTCTTTGACAAAGAAAAGGACGTATTTGTGATGTTCTATGCACCCTGGTCTAAAAAGTGC AAGATGCTGTTCCCACTGTTGGAGGAATTGGGCAGAAAATATCAAAACCACTCCACAATTATCATTGCCA AGATCGATGTCACAGCAAATGACATTCAGCTGATGTACCTGGACCGGTACCCATTCTTCAGGCTGTTCCC CAGCGGCTCTCAACAAGCTGTCCTGTATAAGGGAGAACACACCCTGAAGGGCTTCTCTGACTTCCTGGAA AGCCACATCAAAACTAAGATTGAGGATGAGGATGAGCTGTTGTCTGTTGAGCAAAATGAAGTGATAGAAG AGGAAGTGCTAGCTGAGGAAAAGGAGGTGCCTATGATGAGGAAAGGGTTACCTGAACAGCAGTCGCCTGA GCTGGAGAACATGACCAAGTACGTATCCAAGCTGGAAGAGCCCGCTGGGAAGAAGAAAACATCTGAGGAG GTGGTGGTGGTGGTGGCTAAGCCAAAGGGACCTCCAGTGCAAAAGAAGAAACCAAAAGTCAAGGAAGAAC TT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206717 protein sequence
Red=Cloning site Green=Tags(s) MDLLWMPLLLVAACVSAVHSSPEVNAGVSSIHITKPVHILEERSLLVLTPAGLTQMLNQTRFLMVLFHNP SSKQSRNLAEELGKAVEIMGKGKNGIGFGKVDITIEKELQQEFGITKAPELKLFFEGNRSEPISCKGVVE SAALVVWLRRQISQKAFLFNSSEQVAEFVISRPLVIVGFFQDLEEEVAELFYDVIKDFPELTFGVITIGN VIGRFHVTLDSVLVFKKGKIVNRQKLINDSTNKQELNRVIKQHLTDFVIEYNTENKDLISELHIMSHMLL FVSKSSESYGIIIQHYKLASKEFQNKILFILVDADEPRNGRVFKYFRVTEVDIPSVQILNLSSDARYKMP SDDITYESLKKFGRSFLSKNATKHQSSEEIPKYWDQGLVKQLVGKNFNVVVFDKEKDVFVMFYAPWSKKC KMLFPLLEELGRKYQNHSTIIIAKIDVTANDIQLMYLDRYPFFRLFPSGSQQAVLYKGEHTLKGFSDFLE SHIKTKIEDEDELLSVEQNEVIEEEVLAEEKEVPMMRKGLPEQQSPELENMTKYVSKLEEPAGKKKTSEE VVVVVAKPKGPPVQKKKPKVKEEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_174924 |
| ORF Size | 1752 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_174924.2 |
| RefSeq Size | 2195 bp |
| RefSeq ORF | 1755 bp |
| Locus ID | 204474 |
| UniProt ID | Q8N807 |
| Protein Families | Druggable Genome |
| MW | 66.7 kDa |
| Gene Summary | This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has has an N-terminal ER-signal sequence, two thioredoxin (TRX) domains with non-classical Ser-Lys-Gln-Ser and Ser-Lys-Lys-Cys motifs, respectively, two TRX-like domains, and a C-terminal ER-retention sequence. The protein lacks oxidoreductase activity in vitro and probably functions as a chaperone. This gene's expression appears to be limited to the testis. [provided by RefSeq, Dec 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206717L3 | Lenti ORF clone of Human protein disulfide isomerase-like, testis expressed (PDILT), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206717L4 | Lenti ORF clone of Human protein disulfide isomerase-like, testis expressed (PDILT), mGFP tagged |
CNY 5,890.00 |
|
| RG206717 | PDILT (tGFP-tagged) - Human protein disulfide isomerase-like, testis expressed (PDILT) |
CNY 4,370.00 |
|
| SC306958 | PDILT (untagged)-Human protein disulfide isomerase-like, testis expressed (PDILT) |
CNY 4,360.00 |
