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PPP3CB (NM_021132) Human Tagged ORF Clone
CAT＃: RC206982
PPP3CB (Myc-DDK-tagged)-Human protein phosphatase 3, catalytic subunit, beta isozyme (PPP3CB), transcript variant 2
ORF Plasmid: tGFP
"NM_021132" in other vectors (6)
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CNY 3,912.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CALNA2; CALNB; CNA2; PP2Bbeta |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206982 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCCCCGGAGCCGGCCCGGGCTGCACCGCCCCCACCCCCGCCCCCGCCGCCCCCTCCCGGGGCTG ACCGCGTCGTCAAAGCTGTCCCTTTCCCCCCAACACATCGCTTGACATCTGAAGAAGTATTTGATTTGGA TGGGATACCCAGGGTTGATGTTCTGAAGAACCACTTGGTGAAAGAAGGTCGAGTAGATGAAGAAATTGCG CTTAGAATTATCAATGAGGGTGCTGCCATCCTTCGGAGAGAGAAAACCATGATAGAAGTAGAAGCTCCAA TCACAGTGTGTGGTGACATCCATGGCCAATTTTTTGATCTGATGAAACTTTTTGAAGTAGGAGGATCACC TGCTAATACACGATACCTTTTTCTTGGCGATTATGTGGACAGAGGTTATTTTAGTATAGAGTGTGTCTTA TATTTATGGGTTCTGAAGATTCTATACCCAAGCACATTATTTCTTCTGAGAGGCAACCATGAATGCAGAC ACCTTACTGAATATTTTACCTTTAAGCAGGAATGTAAAATTAAGTATTCGGAAAGAGTCTATGAAGCTTG TATGGAAGCTTTTGATAGTTTGCCTCTTGCTGCACTTTTAAACCAACAGTTTCTTTGTGTTCATGGTGGA CTTTCACCAGAAATACACACACTGGATGATATTAGGAGATTAGATAGATTCAAAGAGCCACCTGCATTTG GACCAATGTGTGACTTGTTATGGTCCGATCCTTCTGAAGATTTTGGAAATGAAAAATCACAGGAACATTT TAGTCACAATACAGTTCGAGGATGTTCTTATTTTTATAACTATCCAGCAGTGTGTGAATTTTTGCAAAAC AATAATTTGTTATCGATTATTAGAGCTCATGAAGCTCAAGATGCAGGCTATAGAATGTACAGAAAAAGTC AAACTACAGGGTTCCCTTCATTAATAACAATTTTTTCGGCACCTAATTACTTAGATGTCTACAATAATAA AGCTGCTGTATTAAAGTATGAAAATAATGTGATGAATATTCGACAGTTTAACTGTTCTCCACATCCTTAC TGGTTGCCTAATTTTATGGATGTCTTCACGTGGTCTTTACCGTTTGTTGGAGAAAAAGTGACAGAAATGT TGGTAAATGTTCTGAGTATTTGCTCTGATGATGAACTAATGACTGAAGGTGAAGACCAGTTTGATGTAGG TTCAGCTGCAGCCCGGAAAGAAATCATAAGAAACAAAATTCGAGCAATTGGCAAGATGGCAAGAGTCTTC TCTGTTCTCAGGGAGGAGAGTGAAAGTGTGCTGACACTCAAGGGCCTGACTCCCACAGGGATGTTGCCTA GTGGAGTGTTAGCTGGAGGACGGCAGACCCTGCAAAGTGCCACAGTTGAGGCTATTGAGGCTGAAAAAGC AATACGAGGATTCTCTCCACCACATAGAATCTGCAGTTTTGAAGAGGCAAAGGGTTTGGATAGGATCAAT GAGAGAATGCCACCTCGGAAAGATGCTGTACAGCAAGATGGTTTCAATTCTCTGAACACCGCACATGCCA CTGAGAACCACGGGACGGGCAACCATACTGCCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206982 protein sequence
Red=Cloning site Green=Tags(s) MAAPEPARAAPPPPPPPPPPPGADRVVKAVPFPPTHRLTSEEVFDLDGIPRVDVLKNHLVKEGRVDEEIA LRIINEGAAILRREKTMIEVEAPITVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVL YLWVLKILYPSTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYEACMEAFDSLPLAALLNQQFLCVHGG LSPEIHTLDDIRRLDRFKEPPAFGPMCDLLWSDPSEDFGNEKSQEHFSHNTVRGCSYFYNYPAVCEFLQN NNLLSIIRAHEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPY WLPNFMDVFTWSLPFVGEKVTEMLVNVLSICSDDELMTEGEDQFDVGSAAARKEIIRNKIRAIGKMARVF SVLREESESVLTLKGLTPTGMLPSGVLAGGRQTLQSATVEAIEAEKAIRGFSPPHRICSFEEAKGLDRIN ERMPPRKDAVQQDGFNSLNTAHATENHGTGNHTAQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021132 |
| ORF Size | 1575 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021132.4 |
| RefSeq Size | 3537 bp |
| RefSeq ORF | 1575 bp |
| Locus ID | 5532 |
| UniProt ID | P16298 |
| Domains | Metallophos, PP2Ac |
| Protein Families | Druggable Genome, Phosphatase, Transcription Factors |
| Protein Pathways | Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Axon guidance, B cell receptor signaling pathway, Calcium signaling pathway, Long-term potentiation, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Oocyte meiosis, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway |
| MW | 59.1 kDa |
| Gene Summary | Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals (PubMed:19154138, PubMed:26794871). Dephosphorylates and activates transcription factor NFATC1 (PubMed:19154138). Dephosphorylates and inactivates transcription factor ELK1 (PubMed:19154138). Dephosphorylates DARPP32 (PubMed:19154138).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
PPP3CB contributes to poor prognosis through activating nuclear factor of activated T-cells signaling in neuroblastoma
,Shakhova, I;Li, Y;Yu, F;Kaneko, Y;Nakamura, Y;Ohira, M;Izumi, H;Mae, T;Varfolomeeva, SR;Rumyantsev, AG;Nakagawara, A;,
Mol. Carcinog.
,PubMed ID 30457174
[PPP3CB]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206982L1 | Lenti ORF clone of Human protein phosphatase 3, catalytic subunit, beta isozyme (PPP3CB), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206982L2 | Lenti ORF clone of Human protein phosphatase 3, catalytic subunit, beta isozyme (PPP3CB), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC206982L3 | Lenti ORF clone of Human protein phosphatase 3, catalytic subunit, beta isozyme (PPP3CB), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206982L4 | Lenti ORF clone of Human protein phosphatase 3, catalytic subunit, beta isozyme (PPP3CB), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG206982 | PPP3CB (tGFP-tagged) - Human protein phosphatase 3, catalytic subunit, beta isozyme (PPP3CB), transcript variant 2 |
CNY 4,370.00 |
|
| SC112977 | PPP3CB (untagged)-Human protein phosphatase 3, catalytic subunit, beta isozyme (PPP3CB), transcript variant 2 |
CNY 2,950.00 |
