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CACNB3 (NM_000725) Human Tagged ORF Clone
CAT＃: RC207229
- TrueORF®
CACNB3 (Myc-DDK-tagged)-Human calcium channel, voltage-dependent, beta 3 subunit (CACNB3), transcript variant 1
ORF Plasmid: tGFP
"NM_000725" in other vectors (6)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CAB3; CACNLB3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207229 representing NM_000725
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATGACGACTCCTACGTGCCCGGGTTTGAGGACTCGGAGGCGGGTTCAGCCGACTCCTACACCAGCC GCCCATCTCTGGACTCAGACGTCTCCCTGGAGGAGGACCGGGAGAGTGCCCGGCGTGAAGTAGAGAGCCA GGCTCAGCAGCAGCTCGAAAGGGCCAAGCACAAACCTGTGGCATTTGCGGTGAGGACCAATGTCAGCTAC TGTGGCGTACTGGATGAGGAGTGCCCAGTCCAGGGCTCTGGAGTCAACTTTGAGGCCAAAGATTTTCTGC ACATTAAAGAGAAGTACAGCAATGACTGGTGGATCGGGCGGCTAGTGAAAGAGGGCGGGGACATCGCCTT CATCCCCAGCCCCCAGCGCCTGGAGAGCATCCGGCTCAAACAGGAGCAGAAGGCCAGGAGATCTGGGAAC CCTTCCAGCCTGAGTGACATTGGCAACCGACGCTCCCCTCCGCCATCTCTAGCCAAGCAGAAGCAAAAGC AGGCGGAACATGTTCCCCCATATGACGTGGTGCCCTCCATGCGGCCTGTGGTGCTGGTGGGACCATCTCT GAAAGGTTATGAGGTCACAGACATGATGCAGAAGGCTCTCTTCGACTTCCTCAAACACAGATTTGATGGC AGGATCTCCATCACCCGAGTCACAGCCGACCTCTCCCTGGCAAAGCGATCTGTGCTCAACAATCCGGGCA AGAGGACCATCATTGAGCGCTCCTCTGCCCGCTCCAGCATTGCGGAAGTGCAGAGTGAGATCGAGCGCAT ATTTGAGCTGGCCAAATCCCTGCAGCTAGTAGTGTTGGACGCTGACACCATCAACCACCCAGCACAGCTG GCCAAGACCTCGCTGGCCCCCATCATCGTCTTTGTCAAAGTGTCCTCACCAAAGGTACTCCAGCGTCTCA TTCGCTCCCGGGGGAAGTCACAGATGAAGCACCTGACCGTACAGATGATGGCATATGATAAGCTGGTTCA GTGCCCACCGGAGTCATTTGATGTGATTCTGGATGAGAACCAGCTGGAGGATGCCTGTGAGCACCTGGCT GAGTACCTGGAGGTTTACTGGCGGGCCACGCACCACCCAGCCCCTGGCCCCGGACTTCTGGGTCCTCCCA GTGCCATCCCGGGACTTCAGAACCAGCAGCTGCTGGGGGAGCGTGGCGAGGAGCACTCCCCCCTTGAGCG GGACAGCTTGATGCCCTCTGATGAGGCCAGCGAGAGCTCCCGCCAAGCCTGGACAGGATCTTCACAGCGT AGCTCCCGCCACCTGGAGGAGGACTATGCAGATGCCTACCAGGACCTGTACCAGCCTCACCGCCAACACA CCTCGGGGCTGCCTAGTGCTAACGGGCATGACCCCCAAGACCGGCTTCTAGCCCAGGACTCAGAGCACAA CCACAGTGACCGGAACTGGCAGCGCAACCGGCCTTGGCCCAAGGATAGCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207229 representing NM_000725
Red=Cloning site Green=Tags(s) MYDDSYVPGFEDSEAGSADSYTSRPSLDSDVSLEEDRESARREVESQAQQQLERAKHKPVAFAVRTNVSY CGVLDEECPVQGSGVNFEAKDFLHIKEKYSNDWWIGRLVKEGGDIAFIPSPQRLESIRLKQEQKARRSGN PSSLSDIGNRRSPPPSLAKQKQKQAEHVPPYDVVPSMRPVVLVGPSLKGYEVTDMMQKALFDFLKHRFDG RISITRVTADLSLAKRSVLNNPGKRTIIERSSARSSIAEVQSEIERIFELAKSLQLVVLDADTINHPAQL AKTSLAPIIVFVKVSSPKVLQRLIRSRGKSQMKHLTVQMMAYDKLVQCPPESFDVILDENQLEDACEHLA EYLEVYWRATHHPAPGPGLLGPPSAIPGLQNQQLLGERGEEHSPLERDSLMPSDEASESSRQAWTGSSQR SSRHLEEDYADAYQDLYQPHRQHTSGLPSANGHDPQDRLLAQDSEHNHSDRNWQRNRPWPKDSY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000725 |
| ORF Size | 1452 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000725.4 |
| RefSeq Size | 2714 bp |
| RefSeq ORF | 1455 bp |
| Locus ID | 784 |
| UniProt ID | P54284 |
| Domains | Ca_channel_B, GuKc |
| Protein Families | Druggable Genome, Ion Channels: Other |
| Protein Pathways | Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway |
| MW | 54.4 kDa |
| Gene Summary | This gene encodes a regulatory beta subunit of the voltage-dependent calcium channel. Beta subunits are composed of five domains, which contribute to the regulation of surface expression and gating of calcium channels and may also play a role in the regulation of transcription factors and calcium transport. [provided by RefSeq, Oct 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207229L1 | Lenti-ORF clone of CACNB3 (Myc-DDK-tagged)-Human calcium channel, voltage-dependent, beta 3 subunit (CACNB3), transcript variant 1 |
CNY 7,888.00 |
|
| RC207229L2 | Lenti-ORF clone of CACNB3 (mGFP-tagged)-Human calcium channel, voltage-dependent, beta 3 subunit (CACNB3), transcript variant 1 |
CNY 5,890.00 |
|
| RC207229L3 | Lenti-ORF clone of CACNB3 (Myc-DDK-tagged)-Human calcium channel, voltage-dependent, beta 3 subunit (CACNB3), transcript variant 1 |
CNY 5,890.00 |
|
| RC207229L4 | Lenti-ORF clone of CACNB3 (mGFP-tagged)-Human calcium channel, voltage-dependent, beta 3 subunit (CACNB3), transcript variant 1 |
CNY 7,888.00 |
|
| RG207229 | CACNB3 (tGFP-tagged) - Human calcium channel, voltage-dependent, beta 3 subunit (CACNB3), transcript variant 1 |
CNY 7,088.00 |
|
| SC122090 | CACNB3 (untagged)-Human calcium channel, voltage-dependent, beta 3 subunit (CACNB3), transcript variant 1 |
CNY 5,488.00 |
