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PGK2 (NM_138733) Human Tagged ORF Clone
CAT＃: RC207941
PGK2 (Myc-DDK-tagged)-Human phosphoglycerate kinase 2 (PGK2)
ORF Plasmid: tGFP
"NM_138733" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | dJ417L20.2; HEL-S-272; PGKB; PGKPS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207941 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCTTTCTAAGAAGTTGACTTTAGACAAACTGGATGTTAGAGGGAAGCGAGTCATCATGAGAGTAG ACTTCAATGTTCCCATGAAGAAGAACCAGATTACAAACAACCAGAGGATCAAGGCTTCCATCCCAAGCAT CAAGTACTGCCTGGACAATGGAGCCAAGGCAGTAGTTCTTATGAGTCATCTAGGTCGGCCTGATGGTGTT CCCATGCCTGACAAATATTCCTTAGCACCTGTTGCTGTTGAGCTCAAATCCTTGCTGGGCAAGGATGTTC TGTTCCTGAAGGACTGTGTAGGCGCAGAAGTGGAGAAAGCCTGTGCCAACCCAGCTCCTGGTTCAGTCAT CCTGCTGGAGAACCTGCGCTTTCATGTGGAGGAAGAAGGGAAGGGCCAAGATCCCTCTGGAAAGAAGATT AAAGCTGAGCCAGATAAAATAGAAGCCTTCCGAGCATCACTTTCCAAGCTAGGGGACGTCTATGTCAATG ATGCTTTTGGCACTGCACACCGCGCTCATAGTTCCATGGTGGGAGTGAATCTGCCCCATAAAGCATCCGG ATTCTTGATGAAGAAGGAACTAGATTACTTTGCTAAAGCCTTGGAAAACCCAGTGAGACCCTTTCTGGCT ATACTTGGTGGAGCCAAAGTGGCAGACAAGATCCAACTTATCAAAAATATGCTGGACAAAGTCAATGAGA TGATTATTGGTGGTGGAATGGCTTATACCTTCCTTAAGGTACTCAACAACATGGAGATTGGTGCTTCCCT GTTTGATGAAGAGGGAGCCAAGATCGTTAAAGATATCATGGCCAAAGCACAAAAGAATGGTGTAAGGATT ACTTTTCCTGTTGATTTTGTTACTGGGGACAAGTTTGACGAGAACGCTCAGGTTGGAAAAGCCACTGTAG CATCTGGCATATCTCCTGGCTGGATGGGTTTGGACTGTGGTCCTGAGAGCAACAAGAATCATGCTCAAGT TGTGGCTCAAGCAAGGCTAATTGTTTGGAATGGGCCGTTAGGAGTATTTGAATGGGATGCCTTTGCTAAG GGAACCAAAGCCCTCATGGATGAAATTGTGAAAGCCACTTCCAAGGGCTGCATCACTGTTATAGGGGGTG GAGACACTGCTACTTGCTGTGCCAAATGGAACACTGAAGATAAAGTCAGCCATGTCAGCACTGGAGGCGG TGCCAGTCTAGAGCTTCTGGAAGGTAAAATCCTTCCTGGAGTAGAGGCCCTCAGCAACATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207941 protein sequence
Red=Cloning site Green=Tags(s) MSLSKKLTLDKLDVRGKRVIMRVDFNVPMKKNQITNNQRIKASIPSIKYCLDNGAKAVVLMSHLGRPDGV PMPDKYSLAPVAVELKSLLGKDVLFLKDCVGAEVEKACANPAPGSVILLENLRFHVEEEGKGQDPSGKKI KAEPDKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPHKASGFLMKKELDYFAKALENPVRPFLA ILGGAKVADKIQLIKNMLDKVNEMIIGGGMAYTFLKVLNNMEIGASLFDEEGAKIVKDIMAKAQKNGVRI TFPVDFVTGDKFDENAQVGKATVASGISPGWMGLDCGPESNKNHAQVVAQARLIVWNGPLGVFEWDAFAK GTKALMDEIVKATSKGCITVIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKILPGVEALSNM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138733 |
| ORF Size | 1251 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138733.5 |
| RefSeq Size | 1721 bp |
| RefSeq ORF | 1254 bp |
| Locus ID | 5232 |
| UniProt ID | P07205 |
| Protein Families | Druggable Genome |
| Protein Pathways | Glycolysis / Gluconeogenesis, Metabolic pathways |
| MW | 44.8 kDa |
| Gene Summary | This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.[provided by RefSeq, May 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207941L3 | Lenti ORF clone of Human phosphoglycerate kinase 2 (PGK2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207941L4 | Lenti ORF clone of Human phosphoglycerate kinase 2 (PGK2), mGFP tagged |
CNY 5,890.00 |
|
| RG207941 | PGK2 (tGFP-tagged) - Human phosphoglycerate kinase 2 (PGK2) |
CNY 4,370.00 |
|
| SC125888 | PGK2 (untagged)-Human phosphoglycerate kinase 2 (PGK2) |
CNY 2,950.00 |
