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LAP2 (TMPO) (NM_001032283) Human Tagged ORF Clone
CAT＃: RC208781
TMPO (Myc-DDK-tagged)-Human thymopoietin (TMPO), transcript variant 2
ORF Plasmid: tGFP
"NM_001032283" in other vectors (6)
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CNY 5,488.00
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CMD1T; LAP2; LEMD4; PRO0868; TP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208781 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGAGTTCCTGGAAGACCCCTCGGTCCTGACAAAAGACAAGTTGAAGAGTGAGTTGGTCGCCAACA ATGTGACGCTGCCGGCCGGGGAGCAGCGCAAAGACGTGTACGTCCAGCTCTACCTGCAGCACCTCACGGC TCGCAACCGGCCGCCGCTCCCCGCCGGCACCAACAGCAAGGGGCCCCCGGACTTCTCCAGTGACGAAGAG CGCGAGCCCACCCCGGTCCTCGGCTCTGGGGCCGCCGCCGCGGGCCGGAGCCGAGCAGCCGTCGGCAGGA AAGCCACAAAAAAAACTGATAAACCCAGACAAGAAGATAAAGATGATCTAGATGTAACAGAGCTCACTAA TGAAGATCTTTTGGATCAGCTTGTGAAATACGGAGTGAATCCTGGTCCTATTGTGGGAACAACCAGGAAG CTATATGAGAAAAAGCTTTTGAAACTGAGGGAACAAGGAACAGAATCAAGATCTTCTACTCCTCTGCCAA CAATTTCTTCTTCAGCAGAAAATACAAGGCAGAATGGAAGTAATGATTCTGACAGATACAGTGACAATGA AGAAGACTCTAAAATAGAGCTCAAGCTTGAGAAGAGAGAACCACTAAAGGGCAGAGCAAAGACTCCAGTA ACACTCAAGCAAAGAAGAGTTGAGCACAATCAGAGCTATTCTCAAGCTGGAATAACTGAGACTGAATGGA CAAGTGGATCTTCAAAAGGCGGACCTCTGCAGGCATTAACTAGGGAATCTACAAGAGGGTCAAGAAGAAC TCCAAGGAAAAGGGTGGAAACTTCAGAACATTTTCGTATAGATGGTCCAGTAATTTCAGAGAGTACTCCC ATAGCTGAAACTATAATGGCTTCAAGCAACGAATCCTTAGTTGTCAATAGGGTGACTGGAAATTTCAAGC ATGCATCTCCTATTCTGCCAATCACTGAATTCTCAGACATACCCAGAAGAGCACCAAAGAAACCATTGAC AAGAGCTGAAGTGGGAGAAAAAACAGAGGAAAGAAGAGTAGAAAGGGATATTCTTAAGGAAATGTTCCCC TATGAAGCATCTACACCAACAGGAATTAGTGCTAGTTGCCGCAGACCAATCAAAGGGGCTGCAGGCCGGC CATTAGAACTCAGTGATTTCAGGATGGAGGAGTCTTTTTCATCTAAATATGTTCCTAAGTATGTTCCCTT GGCAGATGTCAAGTCAGAAAAGACAAAAAAGGGACGCTCCATTCCCGTATGGATAAAAATTTTGCTGTTT GTTGTTGTGGCAGTTTTTTTGTTTTTGGTCTATCAAGCTATGGAAACCAACCAAGTAAATCCCTTCTCTA ATTTTCTTCATGTTGACCCTAGAAAATCCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208781 protein sequence
Red=Cloning site Green=Tags(s) MPEFLEDPSVLTKDKLKSELVANNVTLPAGEQRKDVYVQLYLQHLTARNRPPLPAGTNSKGPPDFSSDEE REPTPVLGSGAAAAGRSRAAVGRKATKKTDKPRQEDKDDLDVTELTNEDLLDQLVKYGVNPGPIVGTTRK LYEKKLLKLREQGTESRSSTPLPTISSSAENTRQNGSNDSDRYSDNEEDSKIELKLEKREPLKGRAKTPV TLKQRRVEHNQSYSQAGITETEWTSGSSKGGPLQALTRESTRGSRRTPRKRVETSEHFRIDGPVISESTP IAETIMASSNESLVVNRVTGNFKHASPILPITEFSDIPRRAPKKPLTRAEVGEKTEERRVERDILKEMFP YEASTPTGISASCRRPIKGAAGRPLELSDFRMEESFSSKYVPKYVPLADVKSEKTKKGRSIPVWIKILLF VVVAVFLFLVYQAMETNQVNPFSNFLHVDPRKSN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001032283 |
| ORF Size | 1362 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001032283.3 |
| RefSeq Size | 4186 bp |
| RefSeq ORF | 1365 bp |
| Locus ID | 7112 |
| UniProt ID | P42167 |
| Protein Families | Stem cell - Pluripotency, Transmembrane |
| MW | 50.7 kDa |
| Gene Summary | Through alternative splicing, this gene encodes several distinct LEM domain containing protein isoforms. LEM domain proteins include inner nuclear membrane and intranuclear proteins, and are involved in a variety of cellular functions including gene expression, chromatin organization, and replication and cell cycle control. The encoded alpha isoform is broadly diffuse in the nucleus and contains a lamin binding domain, while the beta and gamma isoforms are localized to the nuclear membrane and contain an HDAC3 interaction domain. The distinct isoforms may compete with each other when acting to chaperone other proteins and regulate transcription. [provided by RefSeq, Aug 2019] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208781L1 | Lenti ORF clone of Human thymopoietin (TMPO), transcript variant 2, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC208781L2 | Lenti ORF clone of Human thymopoietin (TMPO), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC208781L3 | Lenti ORF clone of Human thymopoietin (TMPO), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208781L4 | Lenti ORF clone of Human thymopoietin (TMPO), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG208781 | TMPO (tGFP-tagged) - Human thymopoietin (TMPO), transcript variant 2 |
CNY 7,088.00 |
|
| SC302623 | TMPO (untagged)-Human thymopoietin (TMPO), transcript variant 2 |
CNY 7,220.00 |
