EIF4B (NM_001417) Human Tagged ORF Clone
CAT#: RC209955
EIF4B (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4B (EIF4B)
ORF Plasmid: tGFP
"NM_001417" in other vectors (6)
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CNY 3,990.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | EIF-4B; PRO1843 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC209955 representing NM_001417
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCTCAGCAAAAAAGAAGAATAAGAAGGGGAAGACTATCTCCCTAACAGACTTTCTGGCTGAGG ATGGGGGTACTGGTGGAGGAAGCACCTATGTTTCCAAACCAGTCAGCTGGGCTGATGAAACGGATGACCT GGAAGGAGATGTTTCGACCACTTGGCACAGTAACGATGACGATGTGTATAGGGCGCCTCCAATTGACCGT TCCATCCTTCCCACTGCTCCACGGGCTGCTCGGGAACCCAATATCGACCGGAGCCGTCTTCCCAAATCGC CACCCTACACTGCTTTTCTAGGAAACCTACCCTATGATGTTACAGAAGAGTCAATTAAGGAATTCTTTCG AGGATTAAATATCAGTGCAGTGCGTTTACCACGTGAACCCAGCAATCCAGAGAGGTTGAAAGGTTTTGGT TATGCTGAATTTGAGGACCTGGATTCCCTGCTCAGTGCCCTGAGTCTCAATGAAGAGTCTCTAGGTAACA GGAGAATTCGAGTGGACGTTGCTGATCAAGCACAGGATAAAGACAGGGATGATCGTTCTTTTGGCCGTGA TAGAAATCGGGATTCTGACAAAACAGATACAGACTGGAGGGCTCGTCCTGCTACAGACAGCTTTGATGAC TACCCACCTAGAAGAGGTGATGATAGCTTTGGAGACAAGTATCGAGATCGTTATGATTCAGACCGGTATC GGGATGGGTATCGGGATGGGTATCGGGATGGCCCACGCCGGGATATGGATCGATATGGTGGCCGGGATCG CTATGATGACCGAGGCAGCAGAGACTATGATAGAGGCTATGATTCCCGGATAGGCAGTGGCAGAAGAGCA TTTGGCAGTGGGTATCGCAGGGATGATGACTACAGAGGAGGCGGGGACCGCTATGAAGACCGATATGACA GACGGGATGATCGGTCGTGGAGCTCCAGAGATGATTACTCTCGGGATGATTATAGGCGTGATGATAGAGG TCCCCCCCAAAGACCCAAACTGAATCTAAAGCCTCGGAGTACTCCTAAGGAAGATGATTCCTCTGCTAGT ACCTCCCAGTCCACTCGAGCTGCTTCTATCTTTGGAGGGGCAAAGCCTGTTGACACAGCTGCTAGAGAAA GAGAAGTAGAAGAACGGCTACAGAAGGAACAAGAGAAGTTGCAGCGTCAGCTGGATGAGCCAAAACTAGA ACGACGGCCTCGGGAGAGACACCCAAGCTGGCGAAGTGAAGAAACTCAGGAACGGGAACGGTCGAGGACA GGAAGTGAGTCATCACAAACTGGGACCTCCACCACATCTAGCAGAAATGCACGAAGGAGAGAGAGTGAGA AGTCTCTAGAAAATGAAACACTCAATAAGGAGGAAGATTGCCACTCTCCAACTTCTAAACCTCCCAAACC TGATCAGCCCCTAAAGGTAATGCCAGCCCCTCCACCAAAGGAGAATGCTTGGGTGAAGCGAAGTTCTAAC CCTCCTGCTCGATCTCAGAGCTCAGACACAGAGCAGCAGTCCCCTACAAGTGGTGGGGGAAAAGTAGCTC CAGCTCAACCATCTGAGGAAGGACCAGGAAGGAAAGATGAAAATAAAGTAGATGGGATGAATGCCCCAAA AGGCCAAACTGGGAACTCTAGCCGTGGTCCAGGAGACGGAGGGAACAGAGACCACTGGAAGGAGTCAGAT AGGAAAGATGGCAAAAAGGATCAAGACTCCAGATCTGCACCTGAGCCAAAGAAACCTGAGGAAAATCCAG CTTCTAAGTTCAGTTCTGCAAGCAAGTATGCTGCTCTCTCTGTTGATGGTGAAGATGAAAATGAGGGAGA AGATTATGCCGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209955 representing NM_001417
Red=Cloning site Green=Tags(s) MAASAKKKNKKGKTISLTDFLAEDGGTGGGSTYVSKPVSWADETDDLEGDVSTTWHSNDDDVYRAPPIDR SILPTAPRAAREPNIDRSRLPKSPPYTAFLGNLPYDVTEESIKEFFRGLNISAVRLPREPSNPERLKGFG YAEFEDLDSLLSALSLNEESLGNRRIRVDVADQAQDKDRDDRSFGRDRNRDSDKTDTDWRARPATDSFDD YPPRRGDDSFGDKYRDRYDSDRYRDGYRDGYRDGPRRDMDRYGGRDRYDDRGSRDYDRGYDSRIGSGRRA FGSGYRRDDDYRGGGDRYEDRYDRRDDRSWSSRDDYSRDDYRRDDRGPPQRPKLNLKPRSTPKEDDSSAS TSQSTRAASIFGGAKPVDTAAREREVEERLQKEQEKLQRQLDEPKLERRPRERHPSWRSEETQERERSRT GSESSQTGTSTTSSRNARRRESEKSLENETLNKEEDCHSPTSKPPKPDQPLKVMPAPPPKENAWVKRSSN PPARSQSSDTEQQSPTSGGGKVAPAQPSEEGPGRKDENKVDGMNAPKGQTGNSSRGPGDGGNRDHWKESD RKDGKKDQDSRSAPEPKKPEENPASKFSSASKYAALSVDGEDENEGEDYAE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001417 |
ORF Size | 1833 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001417.3, NP_001408.2 |
RefSeq Size | 3885 bp |
RefSeq ORF | 1836 bp |
Locus ID | 1975 |
UniProt ID | P23588 |
Domains | RRM |
Protein Pathways | mTOR signaling pathway |
MW | 69 kDa |
Gene Summary | Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.[UniProtKB/Swiss-Prot Function] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation
,Zhu, BL;Long, Y;Luo, W;Yan, Z;Lai, YJ;Zhao, LG;Zhou, WH;Wang, YJ;Shen, LL;Liu, L;Deng, XJ;Wang, XF;Sun, F;Chen, GJ;,
Brain
,PubMed ID 30596903
[EIF4B]
|
ITCH nuclear translocation and H1.2 polyubiquitination negatively regulate the DNA damage response
,Chang, L;Shen, L;Zhou, H;Gao, J;Pan, H;Zheng, L;Armstrong, B;Peng, Y;Peng, G;Zhou, BP;Rosen, ST;Shen, B;,
Nucleic Acids Res.
,PubMed ID 30517763
[EIF4B]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209955L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 4B (EIF4B), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC209955L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 4B (EIF4B), mGFP tagged |
CNY 5,890.00 |
|
RC209955L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 4B (EIF4B), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC209955L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 4B (EIF4B), mGFP tagged |
CNY 5,890.00 |
|
RG209955 | EIF4B (tGFP-tagged) - Human eukaryotic translation initiation factor 4B (EIF4B) |
CNY 4,370.00 |
|
SC119269 | EIF4B (untagged)-Human eukaryotic translation initiation factor 4B (EIF4B) |
CNY 2,950.00 |