Phospholipase C beta 1 (PLCB1) (NM_015192) Human Tagged ORF Clone

CAT＃: RC210197

PLCB1 (Myc-DDK-tagged)-Human phospholipase C, beta 1 (phosphoinositide-specific) (PLCB1), transcript variant 1

ORF Plasmid: DDK tGFP

Datasheet
SDS
Plasmid Map

  "NM_015192" in other vectors (4)

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CNY 8,464.00

货期*
现货
规格
    • 10 ug

Product images

经常一起买 (5)
PS100001
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00

添加到购物车
CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售
PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

添加到购物车
TA592569S
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

添加到购物车

【特别福利】购一抗正装产品，免费领10ul抗体试用装

TA327224
PLCB1 Rabbit Polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 2,700.00

添加到购物车

热销推荐

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1; PLC-I; PLC154; PLCB1A; PLCB1B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC210197 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGGGGCTCAACCCGGAGTGCACGCCTTGCAACTCAAGCCCGTGTGCGTGTCCGACAGCCTCAAGA
AGGGCACCAAATTCGTCAAGTGGGATGATGACTCAACTATTGTTACTCCAATTATTTTGAGGACTGACCC
TCAGGGATTTTTCTTTTACTGGACAGATCAAAACAAGGAGACAGAGCTACTGGATCTCAGCCTTGTCAAA
GATGCCAGATGTGGGAGACACGCCAAAGCTCCCAAGGACCCCAAATTACGTGAACTTTTGGATGTGGGGA
ACATCGGGCGCCTGGAGCAGCGCATGATCACAGTGGTGTATGGGCCTGACCTCGTGAACATCTCCCATTT
GAATCTCGTGGCTTTCCAAGAAGAAGTGGCCAAGGAATGGACAAATGAGGTTTTCAGTTTGGCAACAAAC
CTGCTGGCCCAAAACATGTCCAGGGATGCATTTCTGGAAAAAGCCTATACTAAACTTAAGCTGCAAGTCA
CTCCAGAAGGGCGTATTCCTCTCAAAAACATATATCGCTTGTTTTCAGCAGATCGGAAGCGAGTTGAAAC
TGCTTTAGAGGCTTGTAGTCTTCCATCTTCAAGGAATGATTCAATACCTCAAGAAGATTTCACTCCAGAA
GTGTACAGAGTTTTCCTCAACAACCTTTGCCCTCGACCTGAAATTGATAACATCTTTTCAGAATTTGGTG
CAAAAAGCAAACCATATCTTACCGTTGATCAGATGATGGATTTTATCAACCTTAAGCAGCGAGATCCTCG
GCTTAATGAAATACTTTATCCACCTCTAAAACAAGAGCAAGTCCAAGTATTGATTGAGAAGTATGAACCC
AACAACAGCCTCGCCAGAAAAGGACAAATATCAGTGGATGGGTTCATGCGCTATCTGAGTGGAGAAGAAA
ACGGAGTCGTTTCACCTGAGAAACTGGATTTGAATGAAGACATGTCTCAGCCCCTTTCTCACTATTTCAT
TAATTCCTCGCACAACACCTACCTCACAGCTGGCCAACTGGCTGGAAACTCCTCTGTTGAGATGTATCGC
CAAGTGCTCCTGTCTGGTTGTCGCTGTGTGGAGCTGGACTGCTGGAAGGGACGGACTGCAGAAGAGGAAC
CTGTCATCACCCATGGCTTCACCATGACAACTGAAATATCTTTCAAGGAAGTGATAGAAGCAATTGCGGA
GTGTGCATTTAAGACTTCACCTTTTCCAATTCTCCTTTCGTTTGAGAACCATGTGGATTCCCCAAAGCAG
CAAGCCAAGATGGCGGAGTACTGCCGACTGATCTTTGGGGATGCCCTTCTCATGGAGCCCCTGGAAAAAT
ATCCACTGGAATCTGGAGTTCCTCTTCCAAGCCCTATGGATTTAATGTATAAAATTTTGGTGAAAAATAA
GAAGAAATCACACAAGTCATCAGAAGGAAGCGGCAAAAAGAAGCTCTCAGAACAAGCCTCCAACACCTAC
AGTGACTCCTCCAGCATGTTCGAGCCCTCATCCCCAGGAGCCGGAGAAGCTGATACGGAAAGTGACGACG
ACGATGATGATGATGACTGTAAAAAATCTTCAATGGATGAGGGGACTGCTGGAAGTGAGGCTATGGCCAC
AGAAGAAATGTCTAATCTGGTGAACTATATTCAGCCAGTCAAGTTTGAGTCATTTGAAATTTCAAAAAAA
AGAAATAAAAGTTTTGAAATGTCTTCCTTCGTGGAAACCAAAGGACTTGAACAACTCACCAAGTCTCCAG
TGGAATTTGTAGAATATAACAAAATGCAGCTTAGCAGGATATATCCAAAAGGAACACGTGTGGATTCATC
CAACTATATGCCTCAGCTCTTCTGGAATGCAGGTTGTCAGATGGTGGCACTTAATTTCCAGACAATGGAC
CTGGCTATGCAAATAAATATGGGGATGTATGAATACAACGGGAAGAGTGGCTACAGATTGAAGCCAGAGT
TCATGAGGAGGCCTGACAAGCATTTTGATCCATTTACTGAAGGCATCGTAGATGGGATAGTGGCAAACAC
TTTGTCTGTTAAGATTATTTCAGGTCAGTTTCTTTCTGATAAGAAAGTTGGGACTTACGTGGAAGTAGAT
ATGTTTGGTTTGCCTGTGGATACAAGGAGGAAGGCATTTAAGACCAAAACATCCCAAGGAAATGCTGTGA
ATCCTGTCTGGGAAGAAGAACCTATTGTGTTCAAAAAGGTGGTTCTTCCTACTCTGGCCTGTTTGAGAAT
AGCAGTTTATGAAGAAGGAGGTAAATTCATTGGCCACCGTATCTTGCCAGTGCAAGCCATTCGGCCAGGC
TATCACTATATCTGTCTAAGGAATGAAAGGAACCAGCCTCTGACGCTGCCTGCTGTCTTTGTCTACATAG
AAGTGAAAGACTATGTGCCAGACACATATGCAGATGTCATCGAAGCTTTATCAAACCCAATCCGATATGT
GAACCTGATGGAACAGAGAGCTAAGCAATTGGCTGCTTTGACACTGGAAGATGAAGAAGAAGTAAAGAAA
GAGGCTGATCCTGGAGAAACACCATCAGAGGCTCCAAGTGAAGCGAGAACGACTCCAGCAGAAAATGGGG
TGAATCACACTACAACCCTGACACCCAAGCCACCCTCCCAGGCTCTCCACAGCCAGCCAGCTCCAGGTTC
TGTAAAGGCACCTGCCAAAACAGAAGATCTTATTCAGAGTGTCTTAACAGAAGTGGAAGCACAGACCATC
GAAGAACTAAAGCAACAGAAATCGTTTGTGAAACTTCAAAAGAAACACTACAAAGAAATGAAAGACCTGG
TTAAGAGACACCACAAGAAAACCACTGACCTTATCAAAGAACACACTACCAAGTATAATGAAATTCAGAA
TGACTACTTGAGAAGGAGAGCCGCTTTGGAAAAGTCCGCCAAAAAGGACAGTAAGAAAAAATCGGAACCC
AGCAGCCCTGATCATGGTTCATCAACGATTGAGCAAGACCTCGCTGCCCTGGATGCTGAAATGACCCAAA
AGTTAATAGACTTGAAGGACAAACAACAGCAGCAGCTGCTTAATCTTCGGCAAGAACAGTATTATAGTGA
AAAATACCAGAAGCGAGAACATATTAAACTGCTTATTCAAAAGTTGACGGATGTCGCAGAAGAGTGTCAG
AACAATCAGTTAAAGAAGCTCAAAGAAATCTGTGAGAAAGAAAAGAAAGAATTAAAGAAGAAAATGGATA
AAAAGAGGCAGGAGAAGATAACAGAAGCTAAATCCAAAGACAAAAGTCAGATGGAAGAGGAGAAGACAGA
GATGATCCGGTCATATATCCAGGAAGTGGTGCAGTATATCAAGAGGCTAGAAGAAGCGCAAAGTAAACGG
CAAGAAAAACTCGTAGAGAAACACAAGGAAATACGTCAGCAGATCCTGGATGAAAAGCCCAAGCTGCAGG
TGGAGCTGGAGCAAGAATACCAAGACAAATTCAAAAGACTGCCCCTCGAGATTTTGGAATTCGTGCAGGA
AGCCATGAAAGGAAAGATCAGTGAAGACAGCAATCACGGTTCTGCCCCTCTCTCCCTGTCCTCAGACCCT
GGAAAAGTGAACCACAAGACTCCCTCCAGTGAGGAGCTGGGAGGAGACATCCCAGGAAAAGAATTTGATA
CTCCTCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>RC210197 protein sequence
Red=Cloning site Green=Tags(s)

MAGAQPGVHALQLKPVCVSDSLKKGTKFVKWDDDSTIVTPIILRTDPQGFFFYWTDQNKETELLDLSLVK
DARCGRHAKAPKDPKLRELLDVGNIGRLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATN
LLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPE
VYRVFLNNLCPRPEIDNIFSEFGAKSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEP
NNSLARKGQISVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYR
QVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQ
QAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTY
SDSSSMFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKK
RNKSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTMD
LAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVD
MFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPTLACLRIAVYEEGGKFIGHRILPVQAIRPG
YHYICLRNERNQPLTLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKK
EADPGETPSEAPSEARTTPAENGVNHTTTLTPKPPSQALHSQPAPGSVKAPAKTEDLIQSVLTEVEAQTI
EELKQQKSFVKLQKKHYKEMKDLVKRHHKKTTDLIKEHTTKYNEIQNDYLRRRAALEKSAKKDSKKKSEP
SSPDHGSSTIEQDLAALDAEMTQKLIDLKDKQQQQLLNLRQEQYYSEKYQKREHIKLLIQKLTDVAEECQ
NNQLKKLKEICEKEKKELKKKMDKKRQEKITEAKSKDKSQMEEEKTEMIRSYIQEVVQYIKRLEEAQSKR
QEKLVEKHKEIRQQILDEKPKLQVELEQEYQDKFKRLPLEILEFVQEAMKGKISEDSNHGSAPLSLSSDP
GKVNHKTPSSEELGGDIPGKEFDTPL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_015192
ORF Size 3648 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_015192.4
RefSeq Size 7103 bp
RefSeq ORF 3651 bp
Locus ID 23236
UniProt ID Q9NQ66
Domains C2, PI-PLC-X, PI-PLC-Y
Protein Families Druggable Genome
Protein Pathways Alzheimer's disease, Calcium signaling pathway, Chemokine signaling pathway, Gap junction, GnRH signaling pathway, Huntington's disease, Inositol phosphate metabolism, Long-term depression, Long-term potentiation, Melanogenesis, Metabolic pathways, Phosphatidylinositol signaling system, Vascular smooth muscle contraction, Wnt signaling pathway
MW 138.6 kDa
Gene Summary The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. This gene is activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

SKU Description Size Price
RC210197L3 Lenti ORF clone of Human phospholipase C, beta 1 (phosphoinositide-specific) (PLCB1), transcript variant 1, Myc-DDK-tagged
    • 10 ug

CNY 14,060.00

RC210197L4 Lenti ORF clone of Human phospholipase C, beta 1 (phosphoinositide-specific) (PLCB1), transcript variant 1, mGFP tagged
    • 10 ug

CNY 14,060.00

RG210197 PLCB1 (tGFP-tagged) - Human phospholipase C, beta 1 (phosphoinositide-specific) (PLCB1), transcript variant 1
    • 10 ug

CNY 10,064.00

SC309945 PLCB1 (untagged)-Human phospholipase C, beta 1 (phosphoinositide-specific) (PLCB1), transcript variant 1
    • 10 ug

CNY 8,472.00

You may also need
plasmid icon Empty Vector
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Expression Plasmids
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CRISPR
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