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TRF41 (PAPD7) (NM_006999) Human Tagged ORF Clone
CAT＃: RC210285
- TrueORF®
PAPD7 (Myc-DDK-tagged)-Human PAP associated domain containing 7 (PAPD7), transcript variant 1
ORF Plasmid: tGFP
"NM_006999" in other vectors (6)
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CNY 6,056.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | LAK-1; LAK1; PAPD7; POLK; POLS; TRF4; TRF4-1; TRF41; TUTASE5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210285 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCCTTGTCCTGAAGAAGCAGCTATGAGAAGAGAGGTGGTGAAACGGATCGAAACTGTGGTGAAAG ACCTTTGGCCGACGGCTGATGTACAGATATTTGGCAGCTTTAGTACAGGTCTTTATCTTCCAACTAGCGA CATAGACCTGGTGGTCTTCGGGAAATGGGAGCGTCCTCCTTTACAGCTGCTGGAGCAAGCCCTGCGGAAG CACAACGTGGCTGAGCCGTGTTCCATCAAAGTCCTTGACAAGGCTACGGTACCAATAATAAAGCTCACAG ATCAGGAGACTGAAGTGAAAGTTGACATCAGCTTTAACATGGAGACGGGCGTCCGGGCAGCGGAGTTCAT CAAGAATTACATGAAGAAATATTCATTGCTGCCTTACTTGATTTTAGTATTGAAACAGTTCCTTCTGCAG AGGGACCTGAATGAAGTTTTTACAGGTGGAATTAGCTCATACAGCCTAATTTTAATGGCCATTAGCTTTC TACAGTTGCATCCAAGAATTGATGCCCGGAGAGCTGATGAAAACCTTGGAATGCTTCTTGTAGAATTTTT TGAACTCTATGGGAGAAATTTTAATTACTTGAAAACCGGTATTAGAATCAAAGAAGGAGGTGCCTATATC GCCAAAGAGGAGATCATGAAAGCCATGACCAGCGGGTACAGACCGTCGATGCTGTGCATTGAGGACCCCC TGCTGCCAGGGAATGACGTTGGCCGGAGCTCCTATGGCGCCATGCAGGTGAAGCAGGTCTTCGATTATGC CTACATAGTGCTCAGCCATGCCGTGTCACCGCTGGCCAGGTCCTATCCAAACAGAGACGCCGAAAGTACT TTAGGAAGAATCATCAAAGTAACTCAGGAGGTGATTGACTACCGGAGGTGGATCAAAGAGAAGTGGGGCA GCAAAGCCCACCCGTCGCCAGGCATGGACAGCAGGATCAAGATCAAAGAGCGAATAGCCACATGCAATGG GGAGCAGACGCAGAACCGAGAGCCCGAGTCTCCCTATGGCCAGCGCTTGACTTTGTCGCTGTCCAGCCCC CAGCTCCTGTCTTCAGGCTCCTCGGCCTCTTCTGTGTCTTCACTTTCTGGGAGTGACGTTGATTCAGACA CACCGCCCTGCACAACGCCCAGTGTTTACCAGTTCAGTCTGCAAGCGCCAGCTCCTCTCATGGCCGGCTT ACCCACCGCCTTGCCAATGCCCAGTGGCAAACCTCAGCCCACCACTTCCAGAACACTGATCATGACAACC AACAATCAGACCAGGTTTACTATACCTCCACCGACCCTAGGGGTTGCTCCTGTTCCTTGCAGACAAGCTG GTGTAGAAGGAACTGCGTCTTTGAAAGCCGTCCACCACATGTCTTCCCCGGCCATTCCCTCAGCGTCCCC CAACCCGCTCTCGAGCCCTCATCTGTATCATAAGCAGCACAACGGCATGAAACTGTCCATGAAGGGCTCT CACGGCCACACCCAAGGCGGCGGCTACAGCTCTGTGGGTAGCGGAGGTGTGCGGCCCCCTGTGGGCAACA GGGGACACCACCAGTATAACCGCACCGGCTGGAGGAGGAAAAAACACACACACACACGGGACAGTCTGCC CGTGAGCCTCAGCAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210285 protein sequence
Red=Cloning site Green=Tags(s) MSPCPEEAAMRREVVKRIETVVKDLWPTADVQIFGSFSTGLYLPTSDIDLVVFGKWERPPLQLLEQALRK HNVAEPCSIKVLDKATVPIIKLTDQETEVKVDISFNMETGVRAAEFIKNYMKKYSLLPYLILVLKQFLLQ RDLNEVFTGGISSYSLILMAISFLQLHPRIDARRADENLGMLLVEFFELYGRNFNYLKTGIRIKEGGAYI AKEEIMKAMTSGYRPSMLCIEDPLLPGNDVGRSSYGAMQVKQVFDYAYIVLSHAVSPLARSYPNRDAEST LGRIIKVTQEVIDYRRWIKEKWGSKAHPSPGMDSRIKIKERIATCNGEQTQNREPESPYGQRLTLSLSSP QLLSSGSSASSVSSLSGSDVDSDTPPCTTPSVYQFSLQAPAPLMAGLPTALPMPSGKPQPTTSRTLIMTT NNQTRFTIPPPTLGVAPVPCRQAGVEGTASLKAVHHMSSPAIPSASPNPLSSPHLYHKQHNGMKLSMKGS HGHTQGGGYSSVGSGGVRPPVGNRGHHQYNRTGWRRKKHTHTRDSLPVSLSR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006999 |
| ORF Size | 1626 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006999.2 |
| RefSeq Size | 3871 bp |
| RefSeq ORF | 2379 bp |
| Locus ID | 11044 |
| UniProt ID | Q5XG87 |
| Domains | PAP_assoc, NTP_transf_2 |
| Protein Pathways | RNA degradation |
| MW | 59.9 kDa |
| Gene Summary | The protein encoded by this gene is a DNA polymerase that is likely involved in DNA repair. In addition, the encoded protein may be required for sister chromatid adhesion. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jan 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Air1 Zinc Knuckles 4 and 5 and a Conserved IWRXY Motif Are Critical for the Function and Integrity of the Trf4/5-Air1/2-Mtr4 Polyadenylation (TRAMP) RNA Quality Control Complex
,Milo B. Fasken, Sara W. Leung, Ayan Banerjee, Maja O. Kodani, Ramiro Chavez, Elizabeth A. Bowman, Meghan K. Purohit, Max E. Rubinson, Emily H. Rubinson, and Anita H. Corbett,
J. Biol. Chem., Oct 2011; 286: 37429 - 37445.
[TRF41]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210285L1 | Lenti-ORF clone of PAPD7 (Myc-DDK-tagged)-Human PAP associated domain containing 7 (PAPD7), transcript variant 1 |
CNY 8,456.00 |
|
| RC210285L2 | Lenti-ORF clone of PAPD7 (mGFP-tagged)-Human PAP associated domain containing 7 (PAPD7), transcript variant 1 |
CNY 5,890.00 |
|
| RC210285L3 | Lenti-ORF clone of PAPD7 (Myc-DDK-tagged)-Human PAP associated domain containing 7 (PAPD7), transcript variant 1 |
CNY 5,890.00 |
|
| RC210285L4 | Lenti-ORF clone of PAPD7 (mGFP-tagged)-Human PAP associated domain containing 7 (PAPD7), transcript variant 1 |
CNY 5,890.00 |
|
| RG210285 | PAPD7 (tGFP-tagged) - Human PAP associated domain containing 7 (PAPD7), transcript variant 1 |
CNY 7,656.00 |
|
| SC313926 | PAPD7 (untagged)-Human PAP associated domain containing 7 (PAPD7), transcript variant 1 |
CNY 4,040.00 |
