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STX17 (NM_017919) Human Tagged ORF Clone
CAT＃: RC210324
STX17 (Myc-DDK-tagged)-Human syntaxin 17 (STX17)
ORF Plasmid: tGFP
"NM_017919" in other vectors (7)
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CNY 3,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210324 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGAAGATGAAGAAAAAGTGAAATTACGCCGTCTTGAACCAGCTATCCAGAAATTCATTAAGATAG TAATCCCAACAGACCTGGAAAGGTTAAGAAAGCACCAGATAAATATTGAGAAGTATCAAAGGTGCGGAAT CTGGGACAAGTTGCATGAAGAGCATATCAATGCAGGACGTACAGTTCAGCAACTCCGATCCAATATCCGA GAAATTGAGAAACTTTGTTTGAAAGTCCGAAAGGATGACCTAGTACTTCTGAAGAGAATGATAGATCCTG TTAAAGAAGAAGCATCAGCAGCAACAGCAGAATTTCTCCAACTCCATTTGGAATCTGTAGAAGAACTTAA GAAGCAATTTAATGATGAAGAAACTTTGCTACAGCCTCCTTTGACCAGATCCATGACTGTTGGTGGAGCA TTTCATACTACTGAAGCTGAAGCTAGTTCTCAGAGTTTGACTCAGATATATGCCTTACCTGAAATTCCTC AAGATCAAAATGCTGCAGAATCGTGGGAAACCTTAGAAGCGGACTTAATTGAACTTAGCCAACTGGTCAC TGACTTCTCTCTCCTAGTGAATTCTCAGCAGGAGAAGATTGACAGCATTGCAGACCATGTCAACAGTGCT GCTGTGAATGTTGAAGAGGGAACCAAAAACTTAGGGAAGGCTGCAAAATACAAGCTGGCAGCTCTGCCTG TGGCAGGTGCACTCATCGGGGGAATGGTAGGGGGTCCTATTGGCCTCCTTGCAGGCTTCAAAGTGGCAGG AATTGCAGCTGCACTTGGTGGTGGGGTGTTGGGCTTCACAGGTGGAAAATTGATACAAAGAAAGAAACAG AAAATGATGGAGAAGCTCACTTCCAGCTGTCCAGATCTTCCCAGCCAAACTGACAAGAAATGCAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210324 protein sequence
Red=Cloning site Green=Tags(s) MSEDEEKVKLRRLEPAIQKFIKIVIPTDLERLRKHQINIEKYQRCGIWDKLHEEHINAGRTVQQLRSNIR EIEKLCLKVRKDDLVLLKRMIDPVKEEASAATAEFLQLHLESVEELKKQFNDEETLLQPPLTRSMTVGGA FHTTEAEASSQSLTQIYALPEIPQDQNAAESWETLEADLIELSQLVTDFSLLVNSQQEKIDSIADHVNSA AVNVEEGTKNLGKAAKYKLAALPVAGALIGGMVGGPIGLLAGFKVAGIAAALGGGVLGFTGGKLIQRKKQ KMMEKLTSSCPDLPSQTDKKCS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017919 |
| ORF Size | 906 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017919.3 |
| RefSeq Size | 6910 bp |
| RefSeq ORF | 909 bp |
| Locus ID | 55014 |
| UniProt ID | P56962 |
| Domains | t_SNARE |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | SNARE interactions in vesicular transport |
| MW | 33.3 kDa |
| Gene Summary | SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion (PubMed:23217709, PubMed:25686604, PubMed:28306502). STX17 is a SNARE of the autophagosome involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane (PubMed:23217709, PubMed:25686604, PubMed:28306502). May also play a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment/ERGIC and Golgi and/or regulate transport between the endoplasmic reticulum, the ERGIC and the Golgi (PubMed:21545355).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210324L1 | Lenti ORF clone of Human syntaxin 17 (STX17), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC210324L2 | Lenti ORF clone of Human syntaxin 17 (STX17), mGFP tagged |
CNY 5,890.00 |
|
| RC210324L3 | Lenti ORF clone of Human syntaxin 17 (STX17), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210324L4 | Lenti ORF clone of Human syntaxin 17 (STX17), mGFP tagged |
CNY 5,890.00 |
|
| RG210324 | STX17 (tGFP-tagged) - Human syntaxin 17 (STX17) |
CNY 5,200.00 |
|
| SC113844 | STX17 (untagged)-Human syntaxin 17 (STX17) |
CNY 3,600.00 |
|
| SC322067 | STX17 (untagged)-Human syntaxin 17 (STX17) |
CNY 3,600.00 |
