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EHD1 (NM_006795) Human Tagged ORF Clone
CAT＃: RC211158
EHD1 (Myc-DDK-tagged)-Human EH-domain containing 1 (EHD1)
ORF Plasmid: tGFP
"NM_006795" in other vectors (6)
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CNY 5,960.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | H-PAST; HPAST1; PAST; PAST1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211158 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCAGCTGGGTCAGCAAGGATGCCCGCCGCAAGAAGGAGCCGGAGCTCTTCCAGACGGTGGCCGAGG GGCTGCGGCAGCTGTACGCGCAGAAGCTGCTACCCCTGGAGGAGCACTACCGCTTCCACGAGTTCCACTC GCCCGCGCTGGAGGACGCTGACTTCGACAACAAGCCTATGGTGCTCCTCGTGGGGCAGTACAGCACGGGC AAGACCACCTTCATCCGACACCTGATCGAGCAGGACTTCCCGGGGATGCGCATCGGGCCCGAGCCCACCA CCGACTCCTTCATCGCCGTCATGCACGGCCCCACTGAGGGCGTGGTGCCGGGCAACGCGCTCGTGGTGGA CCCGCGGCGCCCCTTCCGCAAGCTCAACGCGTTTGGCAACGCTTTCCTCAACAGGTTCATGTGTGCCCAG CTGCCCAACCCCGTCCTGGACAGCATCAGCATCATCGACACCCCCGGGATCCTGTCTGGAGAGAAGCAGC GGATCAGCAGAGGCTATGACTTTGCAGCCGTCCTGGAGTGGTTCGCGGAGCGTGTGGACCGCATCATCCT GCTCTTCGACGCCCACAAGCTGGACATCTCCGATGAGTTCTCGGAAGTGATCAAGGCTCTGAAGAACCAT GAGGACAAGATCCGCGTGGTGCTGAACAAGGCAGACCAGATCGAGACGCAGCAGCTGATGCGGGTGTACG GGGCCCTCATGTGGTCCCTGGGCAAGATCATCAACACCCCCGAGGTGGTCAGGGTCTACATCGGCTCCTT CTGGTCCCACCCGCTCCTCATCCCCGACAACCGCAAGCTCTTTGAGGCCGAGGAGCAGGACCTCTTCAAG GACATCCAGTCACTGCCCCGAAACGCCGCCCTCAGGAAGCTCAATGACCTGATCAAGCGGGCACGGCTGG CCAAGGTTCACGCCTACATCATCAGCTCCCTCAAGAAAGAGATGCCCAATGTCTTTGGTAAAGAGAGCAA AAAGAAAGAGCTGGTGAACAACCTGGGAGAGATCTACCAGAAGATTGAGCGCGAGCACCAGATCTCCCCT GGGGACTTCCCGAGCCTCCGCAAGATGCAGGAACTCCTGCAGACCCAGGACTTCAGCAAGTTCCAGGCGC TGAAGCCCAAGCTGCTGGACACGGTGGATGACATGCTGGCCAACGACATCGCGCGGCTGATGGTGATGGT GCGGCAGGAGGAGTCCCTGATGCCTTCCCAGGTGGTCAAGGGCGGCGCCTTTGACGGCACCATGAACGGG CCGTTCGGGCACGGCTACGGCGAGGGGGCCGGCGAGGGCATCGACGACGTGGAGTGGGTGGTGGGCAAGG ACAAGCCCACCTACGACGAGATCTTCTACACGCTGTCCCCTGTCAACGGCAAGATCACGGGCGCCAACGC CAAGAAGGAGATGGTGAAGTCCAAGCTCCCCAACACCGTGCTAGGGAAGATCTGGAAGCTGGCCGACGTG GACAAGGACGGGCTGCTGGACGACGAGGAGTTCGCGCTGGCCAACCACCTCATCAAGGTCAAGCTGGAGG GCCACGAGCTGCCCGCCGACCTGCCCCCGCACCTGGTGCCGCCCTCCAAGCGCAGACATGAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC211158 protein sequence
Red=Cloning site Green=Tags(s) MFSWVSKDARRKKEPELFQTVAEGLRQLYAQKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTG KTTFIRHLIEQDFPGMRIGPEPTTDSFIAVMHGPTEGVVPGNALVVDPRRPFRKLNAFGNAFLNRFMCAQ LPNPVLDSISIIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNH EDKIRVVLNKADQIETQQLMRVYGALMWSLGKIINTPEVVRVYIGSFWSHPLLIPDNRKLFEAEEQDLFK DIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPNVFGKESKKKELVNNLGEIYQKIEREHQISP GDFPSLRKMQELLQTQDFSKFQALKPKLLDTVDDMLANDIARLMVMVRQEESLMPSQVVKGGAFDGTMNG PFGHGYGEGAGEGIDDVEWVVGKDKPTYDEIFYTLSPVNGKITGANAKKEMVKSKLPNTVLGKIWKLADV DKDGLLDDEEFALANHLIKVKLEGHELPADLPPHLVPPSKRRHE SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006795 |
| ORF Size | 1602 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006795.3 |
| RefSeq Size | 3533 bp |
| RefSeq ORF | 1605 bp |
| Locus ID | 10938 |
| UniProt ID | Q9H4M9 |
| Domains | EH, EFh |
| Protein Families | Druggable Genome |
| Protein Pathways | Endocytosis |
| MW | 60.6 kDa |
| Gene Summary | This gene belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The protein encoded by this gene is thought to play a role in the endocytosis of IGF1 receptors. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2013] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
FERARI is required for Rab11-dependent endocytic recycling.
,null,
Nature cell biology
,PubMed ID 31988382
[EHD1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211158L1 | Lenti ORF clone of Human EH-domain containing 1 (EHD1), Myc-DDK-tagged |
CNY 8,360.00 |
|
| RC211158L2 | Lenti ORF clone of Human EH-domain containing 1 (EHD1), mGFP tagged |
CNY 8,360.00 |
|
| RC211158L3 | Lenti ORF clone of Human EH-domain containing 1 (EHD1), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC211158L4 | Lenti ORF clone of Human EH-domain containing 1 (EHD1), mGFP tagged |
CNY 8,360.00 |
|
| RG211158 | EHD1 (tGFP-tagged) - Human EH-domain containing 1 (EHD1) |
CNY 7,560.00 |
|
| SC115854 | EHD1 (untagged)-Human EH-domain containing 1 (EHD1) |
CNY 5,976.00 |
