GABRA2 (NM_000807) Human Tagged ORF Clone

CAT#: RC212430

GABRA2 (Myc-DDK-tagged)-Human gamma-aminobutyric acid (GABA) A receptor, alpha 2 (GABRA2), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_000807" in other vectors (6)

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CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


GABRA2 Rabbit Polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 2,700.00

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms DEE78; EIEE78
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC212430 representing NM_000807
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGACAAAATTGAACATCTACAACATGCAGTTCCTGCTTTTTGTTTTCTTGGTGTGGGACCCTGCCA
GGTTGGTGCTGGCTAACATCCAAGAAGATGAGGCTAAAAATAACATTACCATCTTTACGAGAATTCTTGA
CAGACTTCTGGATGGTTACGATAATCGGCTTAGACCAGGACTGGGAGACAGTATTACTGAAGTCTTCACT
AACATCTACGTGACCAGTTTTGGCCCTGTCTCAGATACAGATATGGAATATACAATTGATGTTTTCTTTC
GACAAAAATGGAAAGATGAACGTTTAAAATTTAAAGGTCCTATGAATATCCTTCGACTAAACAATTTAAT
GGCTAGCAAAATCTGGACTCCAGATACCTTTTTTCACAATGGGAAGAAATCAGTAGCTCATAATATGACA
ATGCCAAATAAGTTGCTTCGAATTCAGGATGATGGGACTCTGCTGTATACCATGAGGCTTACAGTTCAAG
CTGAATGCCCAATGCACTTGGAGGATTTCCCAATGGATGCTCATTCATGTCCTCTGAAATTTGGCAGCTA
TGCATATACAACTTCAGAGGTCACTTATATTTGGACTTACAATGCATCTGATTCAGTACAGGTTGCTCCT
GATGGCTCTAGGTTAAATCAATATGACCTGCTGGGCCAATCAATCGGAAAGGAGACAATTAAATCCAGTA
CAGGTGAATATACTGTAATGACAGCTCATTTCCACCTGAAAAGAAAAATTGGGTATTTTGTGATTCAAAC
CTATCTGCCTTGCATCATGACTGTCATTCTCTCCCAAGTTTCATTCTGGCTTAACAGAGAATCTGTGCCT
GCAAGAACTGTGTTTGGAGTAACAACTGTCCTAACAATGACAACTCTAAGCATCAGTGCTCGGAATTCTC
TCCCCAAAGTGGCTTATGCAACTGCCATGGACTGGTTTATTGCTGTTTGTTATGCATTTGTGTTCTCTGC
CCTAATTGAATTTGCAACTGTTAATTACTTCACCAAAAGAGGATGGGCTTGGGATGGGAAGAGTGTAGTA
AATGACAAGAAAAAAGAAAAGGCTTCCGTTATGATACAGAACAACGCTTATGCAGTGGCTGTTGCCAATT
ATGCCCCGAATCTTTCAAAAGATCCAGTTCTCTCCACCATCTCCAAGAGTGCAACCACGCCAGAACCCAA
CAAGAAGCCAGAAAACAAGCCAGCTGAAGCAAAGAAAACTTTCAACAGTGTTAGCAAAATTGACAGAATG
TCCAGAATAGTTTTTCCAGTTTTGTTTGGTACCTTTAATTTAGTTTACTGGGCTACATATTTAAACAGAG
AACCTGTATTAGGGGTCAGTCCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC212430 representing NM_000807
Red=Cloning site Green=Tags(s)

MKTKLNIYNMQFLLFVFLVWDPARLVLANIQEDEAKNNITIFTRILDRLLDGYDNRLRPGLGDSITEVFT
NIYVTSFGPVSDTDMEYTIDVFFRQKWKDERLKFKGPMNILRLNNLMASKIWTPDTFFHNGKKSVAHNMT
MPNKLLRIQDDGTLLYTMRLTVQAECPMHLEDFPMDAHSCPLKFGSYAYTTSEVTYIWTYNASDSVQVAP
DGSRLNQYDLLGQSIGKETIKSSTGEYTVMTAHFHLKRKIGYFVIQTYLPCIMTVILSQVSFWLNRESVP
ARTVFGVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKRGWAWDGKSVV
NDKKKEKASVMIQNNAYAVAVANYAPNLSKDPVLSTISKSATTPEPNKKPENKPAEAKKTFNSVSKIDRM
SRIVFPVLFGTFNLVYWATYLNREPVLGVSP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_000807
ORF Size 1353 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000807.4
RefSeq Size 2189 bp
RefSeq ORF 1356 bp
Locus ID 2555
UniProt ID P47869
Domains Neur_chan_memb, Neur_chan_LBD
Protein Families Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
MW 51.1 kDa
Gene Summary GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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