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RFX5 (NM_001025603) Human Tagged ORF Clone
CAT＃: RC212448
RFX5 (Myc-DDK-tagged)-Human regulatory factor X, 5 (influences HLA class II expression) (RFX5), transcript variant 2
ORF Plasmid: tGFP
"NM_001025603" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212448 representing NM_001025603
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAAGATGAGCCTGATGCTAAGAGCCCCAAGACTGGGGGAAGGGCCCCCCCAGGTGGTGCTGAGG CTGGGGAACCTACCACCCTTCTTCAGAGGCTCCGAGGTACCATTTCCAAGGCCGTGCAGAACAAAGTAGA GGGGATCCTGCAAGATGTACAGAAATTTTCTGACAATGACAAGCTGTATCTCTACCTTCAGCTCCCCTCA GGACCCACCACTGGAGACAAAAGCTCAGAGCCAAGTACACTGAGCAATGAGGAGTACATGTATGCCTATA GGTGGATCCGCAACCACCTGGAAGAGCACACTGACACCTGTCTGCCAAAGCAAAGTGTTTATGATGCCTA TCGGAAGTACTGTGAGAGTCTTGCCTGTTGCCGCCCACTCAGCACAGCCAACTTTGGCAAGATCATCAGA GAGATCTTCCCTGACATCAAAGCTCGAAGGCTTGGTGGCCGGGGCCAGTCCAAATATTGCTACAGTGGCA TAAGGAGGAAGACCTTGGTGTCTATGCCACCCCTGCCTGGACTTGACCTAAAGGGTTCTGAGAGTCCAGA AATGGGCCCAGAAGTAACCCCAGCACCTCGAGATGAACTGGTGGAGGCAGCGTGTGCCCTGACCTGTGAC TGGGCAGAGCGGATCCTGAAACGGTCCTTCAGTTCCATCGTTGAGGTCGCCCGCTTCCTGCTACAGCAGC ATCTCATCTCTGCCCGATCTGCACATGCCCATGTGCTTAAGGCCATGGGGCTTGCTGAAGAGGACGAACA TGCACCTCGGGAACGGTCATCTAAACCAAAGAATGGTTTAGAGAACCCAGAGGGTGGAGCCCACAAGAAG CCAGAGAGACTGGCCCAGCCTCCTAAGGATCTGGAAGCCCGAACTGGGGCCGGTCCTCTCGCACGTGGAG AGCGGAAGAAGAGTGTAGTTGAGAGCTCGGCCCCAGGAGCCAATAACCTGCAGGTTAATGCCCTAGTGGC TCGGCTGCCTCTGCTCCTTCCCCGGGCCCCTCGCTCACTAATTCCGCCAATCCCAGTCTCTCCACCTATT CTGGCCCCCAGGCTTTCTTCAGGTGCCCTGAAAGTGGCTACACTGCCTCTGTCTAGTAGGGCCGGGGCAC CCCCAGCAGCTGTGCCCATCATTAACATGATCTTACCAACTGTTCCTGCTTTGCCTGGACCTGGACCTGG GCCTGGGCGAGCTCCACCTGGGGGACTCACTCAGCCCCGGGGCACAGAGAACAGAGAGGTAGGCATAGGT GGTGACCAAGGACCACATGACAAGGGTGTCAAGAGGACAGCTGAAGTACCTGTGAGTGAGGCCAGTGGGC AGGCTCCACCAGCTAAAGCAGCAAAGCAGGATATAGAGGATACAGCAAGTGATGCCAAAAGGAAACGGGG GCGCCCTCGAAAAAAGTCAGGTGGAAGTGGGGAAAGGAATTCTACCCCTCTCAAGTCAGCAGCTGCCATG GAATCTGCCCAGTCCTCAAGGTTACCATGGGAGACATGGGGCTCAGGAGGGGAAGGCAACTCAGCTGGAG GGGCAGAGAGGCCAGGGCCAATGGGAGAGGCTGAAAAGGGGGCAGTACTTGCCCAGGGTCAGGGAGATGG TACTGTTTCCAAAGGAGGAAGGGGCCCCGGTTCCCAGCATACCAAAGAAGCAGAAGATAAAATTCCCTTG GTCCCCTCAAAAGTGAGTGTCATCAAGGGCAGCAGAAGCCAAAAGGAGGCTTTTCCTTTGGCAAAGGGAG AGGTAGACACTGCACCACAGGGTAATAAAGACTTAAAGGAGCATGTGCTTCAAAGTTCCTTATCCCAGGA GCATAAAGACCCAAAAGCAACACCCCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212448 representing NM_001025603
Red=Cloning site Green=Tags(s) MAEDEPDAKSPKTGGRAPPGGAEAGEPTTLLQRLRGTISKAVQNKVEGILQDVQKFSDNDKLYLYLQLPS GPTTGDKSSEPSTLSNEEYMYAYRWIRNHLEEHTDTCLPKQSVYDAYRKYCESLACCRPLSTANFGKIIR EIFPDIKARRLGGRGQSKYCYSGIRRKTLVSMPPLPGLDLKGSESPEMGPEVTPAPRDELVEAACALTCD WAERILKRSFSSIVEVARFLLQQHLISARSAHAHVLKAMGLAEEDEHAPRERSSKPKNGLENPEGGAHKK PERLAQPPKDLEARTGAGPLARGERKKSVVESSAPGANNLQVNALVARLPLLLPRAPRSLIPPIPVSPPI LAPRLSSGALKVATLPLSSRAGAPPAAVPIINMILPTVPALPGPGPGPGRAPPGGLTQPRGTENREVGIG GDQGPHDKGVKRTAEVPVSEASGQAPPAKAAKQDIEDTASDAKRKRGRPRKKSGGSGERNSTPLKSAAAM ESAQSSRLPWETWGSGGEGNSAGGAERPGPMGEAEKGAVLAQGQGDGTVSKGGRGPGSQHTKEAEDKIPL VPSKVSVIKGSRSQKEAFPLAKGEVDTAPQGNKDLKEHVLQSSLSQEHKDPKATPP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001025603 |
| ORF Size | 1848 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001025603.1, NP_001020774.1 |
| RefSeq Size | 3611 bp |
| RefSeq ORF | 1851 bp |
| Locus ID | 5993 |
| UniProt ID | P48382 |
| Protein Families | Transcription Factors |
| Protein Pathways | Antigen processing and presentation, Primary immunodeficiency |
| MW | 65.1 kDa |
| Gene Summary | A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212448L3 | Lenti ORF clone of Human regulatory factor X, 5 (influences HLA class II expression) (RFX5), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212448L4 | Lenti ORF clone of Human regulatory factor X, 5 (influences HLA class II expression) (RFX5), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG212448 | RFX5 (tGFP-tagged) - Human regulatory factor X, 5 (influences HLA class II expression) (RFX5), transcript variant 2 |
CNY 4,370.00 |
|
| SC302426 | RFX5 (untagged)-Human regulatory factor X, 5 (influences HLA class II expression) (RFX5), transcript variant 2 |
CNY 7,220.00 |
